Analysis and solution of false-positives when testing CVA16 sera using an antibody assay against the EV71 virus

“…EV71 and CA16 are closely related genetically, sharing 77% nucleotide and 89% amino acid homologies. [1,19] Compared with EV71-VP1, EV71-VP1-VP2-VP3 has more epitopes that were the same as CA16 protein. On the other hand, the more VP proteins were coated, the more IgM antibodies in the sample bound to T-line, and the accuracy was higher.…”

Section: Resultsmentioning

confidence: 99%

Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM

Liu¹

2018

AJCEM

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Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus.

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“… 12 – 15 In addition, enzyme-linked immunosorbent assay (ELISA) techniques have been developed to detect antibodies for the clinical diagnosis of HFMD. 16 , 17 The aim of the present study was to develop a rapid, sensitive and more specific and effective diagnostic method for detecting EV71 in clinical specimens.…”

Section: Introductionmentioning

confidence: 99%

Protein microarray-mediated detection of antienterovirus antibodies in serum

Zhang

Xiu²,

Zhang³

et al. 2016

J Int Med Res

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ObjectiveTo utilize prokaryotic gene expression and protein microarray to develop and evaluate a sensitive, accurate protein microarray assay for detecting antienterovirus antibodies in serum samples from patients with hand, foot and mouth disease (HFMD). Enterovirus 71 (EV71) and coxsackievirus A16 (CA16), two common causative agents for HFMD, were used for assay development.MethodsSerum was collected from patients with HFMD and healthy controls. EV71 and CA16 VP1 and VP3 genes were expressed in transfected Escherichia coli; the resultant VP1 and 3 proteins were used in a microarray assay for human serum EV71 and CA16 immunoglobulin (Ig) M and IgG. To validate the microarray assay, serum samples were tested for EV71 IgM using enzyme-linked immunosorbent assay (ELISA).ResultsOut of 50 patients with HFMD, EV71 IgM and CA16 IgM was detected in 80% and 44% of serum samples, respectively, using protein microarray, and EV71 IgM was detected in 78% of samples using ELISA. Protein microarray and ELISA showed 100% specificity for EV71-IgM detection.ConclusionThe protein microarray assay developed in the present study shows potential as a sensitive technique for detecting EV71 IgM in serum samples from patients with HFMD.

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Analysis and solution of false-positives when testing CVA16 sera using an antibody assay against the EV71 virus

Cited by 7 publications

References 15 publications

Hand, foot, and mouth disease: Identifying and managing an acute viral syndrome

Hand, foot, and mouth disease: Identifying and managing an acute viral syndrome

Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM

Protein microarray-mediated detection of antienterovirus antibodies in serum

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