Analysis of 32 toxic natural substances in herbal products by liquid chromatography quadrupole linear ion trap mass spectrometry

Zeng, Yun; Quek, Yi-Ling; Kee, Chee‐Leong; Low, Min‐Yong; Bloodworth, Bosco Chen; Ge, Xiaowei

doi:10.1016/j.jpba.2015.07.005

Cited by 12 publications

(14 citation statements)

References 14 publications

(17 reference statements)

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“…The drugs of abuse originated from plants, such as Δ 9tetrahydrocannabinol and cocaine have been excluded. To our knowledge, only one trial to construct libraries specific to natural toxic substances has been published [6]; but they used low-resolution LC-MS/MS instrument unable to make estimation of the molecular formulae, using its accurate mass numbers, which are very useful for tentative identification of an unknown substances. Recently, Wang et al [23] reported high-throughput screening of more than 200 toxic substances including narcotic drugs, psychotropic drugs, pesticides, natural toxins, and other drugs; however, in their collection, only 3 substances were in common with those in our article.…”

Section: Discussionmentioning

confidence: 99%

“…Forensic toxicology is a part of the pharmacological science, which is concerned with the identification/quantification and effects of various drugs and poisons in human beings [3]. Natural toxins are very important analytical targets in forensic toxicology [4][5][6]. It is difficult to attribute a cause of death to natural toxin(s) in routine toxicological analysis because there is currently no effective routine screening method for a variety of natural toxins [7].…”

Section: Introductionmentioning

confidence: 99%

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Development and application of a forensic toxicological library for identification of 56 natural toxic substances by liquid chromatography–quadrupole time-of-flight mass spectrometry

et al. 2019

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Purpose The present study aims to develop a forensic toxicological library to identify 56 natural toxic substances by liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-QTOF-MS/MS). Methods For setting up the library of product ion spectra, individual substances (31 plant toxins, 7 mushroom toxins, 5 marine toxins, 5 frog venoms, 4 mycotoxins, and 4 substances derived from plants) were analyzed by LC-QTOF-MS/MS with positive and negative ionization. The product ion spectra were acquired at the collision energies (CEs) of 20, 35, and 50 eV in single enhanced product ion mode and then in collision energy spread mode in which the CE ramp range was set to 35 ± 15 eV. Results To test the performance of the library, human blood plasma samples were spiked with a mixture of lycorine and domoic acid, extracted by acetonitrile deproteinization and analyzed by LC-QTOF-MS/MS. Identification by our library search could be achieved for these toxins at the purity scores of 79.1 and 67.2, respectively. The method was also applied to postmortem blood from a death case with an aconite intake, and showed that four toxins in an aconite could be identified in the blood sample at the purity scores of 54.6-60.3. Conclusions This library will be more effective for the screening of natural toxic substances in routine forensic toxicological analysis. To our knowledge, there are no reports dealing with development of library for natural toxic substances by LC-QTOF-MS/MS.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and application of a forensic toxicological library for identification of 56 natural toxic substances by liquid chromatography–quadrupole time-of-flight mass spectrometry

et al. 2019

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“…exhibits 20 distinct GAs with good separation in one chromatogram within 20 min. Recently, a biphenyl HPLC column showed excellent separations of multiple steroids with similar structure . As GAs have similar chemical structure to steroids, it sheds lights on the application of biphenyl HPLC column to GAs analyses in the future.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Progress and development of analytical methods for gibberellins

Pan

Tan

Yong

et al. 2016

J of Separation Science

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Gibberellins, as a group of phytohormones, exhibit a wide variety of bio-functions within plant growth and development, which have been used to increase crop yields. Many analytical procedures, therefore, have been developed for the determination of the types and levels of endogenous and exogenous gibberellins. As plant tissues contain gibberellins in trace amounts (usually at the level of nanogram per gram fresh weight or even lower), the sample pre-treatment steps (extraction, pre-concentration, and purification) for gibberellins are reviewed in details. The primary focus of this comprehensive review is on the various analytical methods designed to meet the requirements for gibberellins analyses in complex matrices with particular emphasis on high-throughput analytical methods, such as gas chromatography, liquid chromatography, and capillary electrophoresis, mostly combined with mass spectrometry. The advantages and drawbacks of the each described analytical method are discussed. The overall aim of this review is to provide a comprehensive and critical view on the different analytical methods nowadays employed to analyze gibberellins in complex sample matrices and their foreseeable trends.

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“…[1] However, some manufacturers have illegally adulterated SHPs with one or more synthetic compounds to reap huge profits. [4,5] It is effective in the prevention of hypotension [6] and is used to treat the symptoms of asthma. [3] Ephedrine (Ep), one of the chemicals frequently found in adulterated SHPs, is an alkaloid derived from various plants in the genus Ephedra (Ma Huang in Chinese).…”

Section: Introductionmentioning

confidence: 99%

“…[3] Ephedrine (Ep), one of the chemicals frequently found in adulterated SHPs, is an alkaloid derived from various plants in the genus Ephedra (Ma Huang in Chinese). [4,5] It is effective in the prevention of hypotension [6] and is used to treat the symptoms of asthma. [7] Pseudoephedrine (Ps) is a milder stimulant than is Ep and is widely used as a nasal decongestant.…”

Section: Introductionmentioning

confidence: 99%

Two‐dimensional correlation infrared spectroscopy applied to the identification of ephedrine and pseudoephedrine in illegally adulterated slimming herbal products

Liu

et al. 2016

Drug Testing and Analysis

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Two-dimensional correlation spectroscopy (2DCOS) was employed for the identification of ephedrine (Ep) and pseudoephedrine (Ps) present in illegally adulterated slimming herbal products (SHPs). Second derivative (SD) spectral pretreatment was used prior to 2DCOS analysis to highlight specific features not readily observable by Fourier transform infrared spectroscopy (FTIR), SD-FTIR, or original 2DCOS, leading to enhanced resolution and a reduced lower limit of detection (<1% in this study). After examining the power spectra of suspicious SHPs, bands containing characteristic peaks for Ep (701, 747, 1042, 1363, 1375, 1451, 1478 cm etc) and/or Ps (703, 767, 1037, 1375, 1428, 1455, 1590 cm 1, etc.) were selected to construct synchronous and asynchronous maps for further analysis, while the latter was applied to discriminate positive SHPs adulterated simultaneously with Ep and Ps. The proposed method is simple and economical and has the potential to identify other chemicals in illegally adulterated herbal products. Copyright © 2016 John Wiley & Sons, Ltd.

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Analysis of 32 toxic natural substances in herbal products by liquid chromatography quadrupole linear ion trap mass spectrometry

Cited by 12 publications

References 14 publications

Development and application of a forensic toxicological library for identification of 56 natural toxic substances by liquid chromatography–quadrupole time-of-flight mass spectrometry

Development and application of a forensic toxicological library for identification of 56 natural toxic substances by liquid chromatography–quadrupole time-of-flight mass spectrometry

Progress and development of analytical methods for gibberellins

Two‐dimensional correlation infrared spectroscopy applied to the identification of ephedrine and pseudoephedrine in illegally adulterated slimming herbal products

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