Analysis of 7-ketocholesterol in low density lipoprotein and fatty acid composition in erythrocyte membranes of patients on maintenance hemodialysis and healthy controls

Tsuzuki, Daisuke; Sumino, Kimiaki; Yokoyama, Mitsuhiro

doi:10.1016/s0009-8981(00)00199-6

Cited by 14 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The membrane lipids of uremic red blood cells have been shown to change quantitatively and qualitatively [24,39], and lipids are widely used in nature as second messengers in pathways leading to gene induction. Recently, it has been shown that PS-liposomes inhibit NO production upstream of the transcription of inducible NO synthase (iNOS) mRNA, and that the inhibition of p38MAP kinase is crucial for this effect [40].…”

Section: Discussionmentioning

confidence: 99%

Adherence of uremic erythrocytes to vascular endothelium decreases endothelial nitric oxide synthase expression

Bonomini¹,

Pandolfi²,

Pietro³

et al. 2005

Kidney International

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Adherence of uremic erythrocytes to vascular endothelium decreases endothelial nitric oxide synthase expression

Bonomini¹,

Pandolfi²,

Pietro³

et al. 2005

Kidney International

View full text Add to dashboard Cite

show abstract

“…Cholesterol and all COPs could be detected in samples of normal brain that had been spiked with minute quantities of these compounds (Figure 4). Ions at mass charge (m/z) ratios 458 (cholesterol TMS), m/z 131 (25-hydroxycholesterol TMS), m/z 472 (7-ketocholesterol TMS), m/z 456 (7␤-hydroxycholesterol TMS), m/z 474(cholesterol 5␣,6␣-epoxide TMS and cholesterol 5␤,6␤-epoxide TMS) and ions at m/z 372 (5␣-cholestane TMS) were used to quantify cholesterol and COPs as previously described (42). A baseline level of cholesterol was detected in homogenates from the hippocampi of saline injected (Table 1) and one-day post-saline injected rats (Table 1).…”

Section: Gas Chromatographic Mass Spectrometric (Gc/ms) Analysis Of Cmentioning

confidence: 98%

Certification of Death in Singapore

Ong

Lai

2014

Proceedings of Singapore Healthcare

View full text Add to dashboard Cite

Little is known about changes in sterols, in particular cholesterol, and cholesterol oxidation products (COPs) in oxidative injury in neural tissues. We have therefore examined changes in cholesterol and COPs using a model of excitotoxic injury. Intracerebroventricular injections of kainate in rats resulted in an increase in immunoreactivity to cholesterol in the affected CA fields of the hippocampus. The increase was confirmed by increased filipin staining of cholesterol in adjacent sections from the same animals, and in hippocampal slice or neuronal cultures after kainate treatment. In neuronal cultures, addition of lovastatin, an inhibitor of cholesterol synthesis, attenuated the increased filipin staining after kainate treatment, indicating that the increase in cholesterol could involve increased cholesterol synthesis. Furthermore, gas chromatographic mass spectrometric (GC/MS) analysis of cholesterol and COPs in kainate-injected rat brain showed a marked increase in cholesterol and COPs including 7-ketocholesterol, 3 days after kainate treatment. The addition of some COPs, including 7-ketocholesterol and cholesterol epoxides to hippocampal slices resulted in neuronal injury as reflected by decreased staining of a neuronal marker in the affected CA fields. The ability of these COPs to produce neuronal injury was attenuated by glutathione, suggesting that oxidative mechanisms are involved in neuronal injury induced by these products. These results, together with GC/MS results that showed significant increase in 7-ketocholesterol at 3 days post-kainate injury suggest that 7-ketocholesterol may be a factor in aggravating oxidative damage to neurons, after the initial stages of kainate-induced neuronal injury.

show abstract

“…These changes also led to an increased ratio of UFA:SFA in the synbiotic group (P < 0.05) over 12 wk, whereas the control showed insignificant changes (Table 5). It has been reported that a decrease in the ratio of UFA:SFA decreases the fluidity of the RBC membrane (Tsuzuki et al, 2000). Red blood cell membrane fluidity is a crucial biophysical property that dictates the mobility and rotational…”

Section: Fatty Acid Methyl Estersmentioning

confidence: 99%

A synbiotic containing Lactobacillus acidophilus CHO-220 and inulin improves irregularity of red blood cells

Ooi

Bhat

Ahmad

et al. 2010

Journal of Dairy Science

View full text Add to dashboard Cite

This randomized, double-blind, placebo-controlled, and parallel-design study was conducted to investigate the effect of a synbiotic product containing Lactobacillus gasseri [corrected] CHO-220 and inulin on the irregularity in shape of red blood cells (RBC) in hypercholesterolemic subjects. The subjects (n=32) were randomly allocated to 2 groups, a treatment group (synbiotic product) and a control group (placebo), and received 4 capsules of either synbiotic or placebo daily for 12 wk. Morphological representation via scanning electron microscopy showed that the occurrence of spur RBC was improved upon supplementation of the synbiotic. In addition, the supplementation of synbiotic reduced the cholesterol:phospholipids ratio of the RBC membrane by 47.02% over 12 wk, whereas the control showed insignificant changes. Our present study also showed that supplementation of the synbiotic reduced the concentration of saturated fatty acids (SFA), increased unsaturated fatty acids (UFA), and increased the ratio of UFA:SFA over 12 wk, whereas the control showed inconspicuous changes. The alteration of RBC membrane was assessed using fluorescence anisotropy (FAn) and fluorescence probes with different affinities for varying sections of the membrane phospholipid bilayer. A noticeable decrease in FAn of three fluorescent probes was observed in the synbiotic group compared with the control over 12 wk, indicative of increased membrane fluidity and reduced cholesterol enrichment in the RBC membrane.

show abstract

Analysis of 7-ketocholesterol in low density lipoprotein and fatty acid composition in erythrocyte membranes of patients on maintenance hemodialysis and healthy controls

Cited by 14 publications

References 39 publications

Adherence of uremic erythrocytes to vascular endothelium decreases endothelial nitric oxide synthase expression

Adherence of uremic erythrocytes to vascular endothelium decreases endothelial nitric oxide synthase expression

Certification of Death in Singapore

A synbiotic containing Lactobacillus acidophilus CHO-220 and inulin improves irregularity of red blood cells

Contact Info

Product

Resources

About