Analysis of amino acids as tert.-butyldimethylsilyl derivatives by gas chromatography

Gas chromatographic enantioseparation of derivatized α-amino acids on chiral stationary phases—Past and present

“…In the achiral domain, three derivatization agents used in GC analysis of ␣-amino acids were compared: N,Obis(trimethylsilyl)trifluoroacetamide (BSTFA), N-methyl-N-(tertbutyldimethylsilyl)trifluoroacetamide (MTBSTFA), and isobutyl chloroformate (iBuCF) [235]. tert-Butyldimethylsilylation (TBDMS) has been used for the derivatization of amino acids for achiral GC-MS analyses [236][237][238][239][240]. In a quest for a straightforward derivatization procedure for the prospected investigation of the enantiomers of ␣-amino acids on Martian samples, a one-step tert-butyldimethylsilylation method has been suggested [241].…”

Analysis of heterocyclic aromatic amines in foods by gas chromatography–mass spectrometry as their tert.-butyldimethylsilyl derivatives

“…The standard or sample residues were always dried under a gentle stream of dry nitrogen and kept overnight over phosphorous pentoxide in vacuum [30] in order to prevent the presence of water. Anhydrous acetonitrile was also used as solvent in the derivatization procedure [29]. Since the amines are particularly prone to adsorption on the glassware surface, silanised vials were used.…”

“…Among these, the sterically crowed tert.-butyldimethylsilyl (TBDMS) group is presently the most versatile for GC analysis. When compared with other silylating reagents, such as the popular trimethylsilyl group, the TBDMS group is up to 10,000 times more stable to hydrolysis, presents enhanced reactivity towards amines, and origins by-products that provide direct injection of the reaction mixture for GC analysis [28,29]. Besides improving volatility and stability, the introduction of the silyl group can also serve to enhance mass spectrometric properties.…”

Capillary gas chromatography of amino acids as their tert‐butyldimethylsilyl derivatives in the analysis of serum amino acids in metabolic diseases

“…Biermann er a/. could not resolve glutamate and arginine [17]. The TBDMS derivatives of amino acids can be prepared in good yields by heating at 150°C for 2 h using acetonitrile as solvent [18].…”