Analysis of bioactivities and chemical composition of Ziziphus joazeiro Mart. using HPLC–DAD

Abstract

The aim of this study was to evaluate the chemical profile and antioxidant, antimicrobial and antiparasitic activities of the hydroalcoholic extract of the leaves of Ziziphus joazeiro Mart. (HELZJ). The antioxidant DPPH and FRAP assays and chemical profile were determined by colorimetric methods and HPLC/DAD. The antiparasitic, antibiotic and antibiotic-modifying activity were evaluated by microdilution assays. The HPLC-DAD assay showed the presence of mostly tannins and flavonoids, such as caffeic acid and quercetin. The levels of polyphenols and flavonoids were 183.136 mg/g extract and 7.37 mg/g extract, respectively. DPPH and FRAP showed low antioxidant activity for the extract. The antibacterial and antifungal activities were not of clinical relevance, showing MIC>1024 μg/mL. However, synergism was observed between HELZJ and the antibiotics amikacin and gentamicin, which resulted in decreased bacterial drug resistance. EHFZJ showed low toxicity in fibroblasts in vitro, while antiparasitic results against Trypnosoma cruzi, Leishmania braziliensis and Leishmania infantum were not clinically relevant. Thus, our results indicate that Z. joazeiro Mart. (HELZJ) could be a source of plant-derived natural products that could lead to the development of promising new antibiotic compounds for infectious diseases.

DNA Protecting Activities of Nymphaea nouchali (Burm. f) Flower Extract Attenuate t-BHP-Induced Oxidative Stress Cell Death through Nrf2-Mediated Induction of Heme Oxygenase-1 Expression by Activating MAP-Kinases

“…A stepwise gradient of solvent A (acetonitrile) to solvent B (1% formic acid solution) was used with the ration changing each minute as follows: 10% A up to 10 min, which was then changed to obtain 30%, 50%, 60%, 90%, 20%, and 10% A in 15, 20, 25, 30, 35, and 40 min, respectively, at λ = 280 nm. This is in accordance with a slightly varied protocol detailed by Brito et al [ 37 ]. Polyphenolic compounds were identified by comparing retention times with those of available pure standards.…”

Mineral Elements Bio-Accessibility and Antioxidant Indices of Blanched Basella rubra at Different Phases of in vitro Gastrointestinal Digestion

“…The limit of detection (LOD) and limit of quantification (LOQ) were calculated based on the standard deviation of the responses and the slope using three independent analytical curves. LOD and LOQ were calculated as 3.3 and 10 σ/S, respectively, where σ is the standard deviation of the response and S is the slope of the calibration curve as defined by Brito et al ( 19 ).…”

Hygroscopic Behavior and Thermodynamic Properties of Ziziphus joazeiro Mart. Kernel Flour

“…(Ziziphus joazeiro Mart), popularly known as juá , is a tropical fruit from the Rhamnaceae Ziziphus plant that is an endemic caatinga species mostly found on the northeastern Brazil (Brito et al, 2015).…”

“…Beyond the use for food, the local population uses the leaves, bark and roots for many other purposes comprising the production of traditional remedies for the treatment of several diseases (Brito et al, 2015). However, despite being a species widely used by the northeastern semiarid population, the exploitation of juazeiro is limited to extractivism (Silva et al, 2018).…”