Analysis of Chemical Components in the Roots of Eight Intersubgeneric Hybrids of Paeonia

Abstract

Paeonia cultivars are famous ornamental plants, and some of them are also traditional Chinese medicinal resources. Intersubgeneric hybrids of Paeonia (IHPs) are formed by the hybridization of herbaceous peony (Paeonia lactiflora) and tree peony (Paeonia×suffruticosa or lutea hybrid tree peony). The phenotypic characteristics of IHPs are similar to those of herbaceous peony, and their root systems are large and vigorous. However, their medicinal value has not been reported yet. In this study, the roots of eight IHP samples were analyzed by high performance liquid chromatography quadrupole time‐of‐flight mass spectrometry (HPLC‐Q‐TOF‐MS/MS). A total of 18 compounds were identified, including phenols, paeonols, monoterpene glycosides, and tannins. The contents of monoterpene glycosides and tannins in IHPs were higher than herbaceous peony and tree peony, exceeding 44.76 mg/g DW and 11.50 mg/g DW, respectively. Three IHPs, ‘Prairie Charm’, ‘Garden Treasure’, and ‘Yellow Emperor’, with more types and a higher content of medicinal compounds, were screened out by cluster analysis. These IHPs have considerable potential for the development of medicinal resources.

Development of DNA Markers for Interspecific and Intersubgeneric Hybrids Involved in Tree Peony Cultivar Groups

“…Intersubgeneric hybrids are of high utility because they combine characteristics of both herbaceous and tree peonies, including high ornamental value, good disease resistance, strong growth potential (Sun et al, 2021), a flowering period similar to that of herbaceous peonies, strong adaptability, and abundant flowers (Yang et al, 2020). Based on the nucleotide sequence of herbaceous peony chloroplast DNA (P. lactiflora chloroplast MK860971) and sequences of HPB primers, the assumed band size amplified by the HPB primer was 395 bp for herbaceous peonies.…”

“…(Stern, 1943;Zhao et al, 2008). Crosses between herbaceous and tree peonies usually yield almost no viable seeds; nevertheless, more than 140 hybrids between herbaceous and tree peony cultivars have been developed by the Itoh crossing method, which obtains intersubgeneric hybrids between herbaceous peony as a seed parent, and progeny of the P. lutea (Lutea hybrid) as a pollen parent (Yang et al, 2020;Sun et al, 2021). Recently, Hong (2021) divided Paeonia into subgenus Moutan for woody plants and subgenus Paeonia for herbaceous plants.…”

Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts

“…Compounds 88 and 89 were reported as constituents of P. lactiflora roots and seeds, respectively [51]. Compounds 87 and 94 were also detected in roots of P. lactiflora [44] and Paeonia daurica [52], respectively. The only compounds found in the petals of herbaceous peonies were 90, 91, 92, 93, and 95, and they were discovered recently by our group, in the petals of P. tenuifolia [25].…”

“…MS 3 base peaks were found at 125 m/z (loss of CO 2 -44 Da). Digallic acid derivatives were discovered as compounds 5 (3.00 min), 11 (3.49 min), 25 (4.64 min), 35 (5.25 min), and 45 (6.49 min), and their fragmentations were recently validated [43,44]. Compounds 9 (3.34 min), 31 (4.89 min), and 36 (5.25 min) all had the same exact masses (183 m/z) and similar fragmentation patterns, indicating that they are isomers of methyl gallate.…”

Functional Analysis of PsARRO−1 in Root Development of Paeonia suffruticosa

“…Therefore, solving the problem of rooting in vitro would represent a breakthrough in Paeonia suffruticosa tissue culture. Many studies have focused on micropropagation [12][13][14][15], rooting cultivation physiology [16,17], the root microenvironment [18][19][20], stress physiology [21], and metabolic processes [5,22,23]. These studies have shown that the root formation process of Paeonia suffruticosa is complex and cannot be explained by a single factor [10,12,14,16,21,22].…”