Analysis of chemical constituents in Cistanche species

Jiang, Yong; Tu, Pengfei

doi:10.1016/j.chroma.2008.07.031

Cited by 144 publications

(105 citation statements)

References 37 publications

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“…Cell survival after radiation was determined using an MTT assay as previously described (10). In brief, 2x10 4 cells were plated in 200 µl culture medium in 96-well plates and incubated for 48 h. At the indicated time points (2,24,48 and 72 h after radiation), a total of 20 µl MTT (Sigma-Aldrich) solution in phosphate-buffered saline was added to each well. After 4 h of incubation, the supernatant was removed and 150 µl dimethyl-sulfoxide was added to each well to terminate the reaction.…”

Section: Methodsmentioning

confidence: 99%

“…Cistanche, a genus of parasitic plants mainly distributed in arid lands and desert, have been commonly used as a traditional Chinese herbal medicine for treating various disorders, including renal deficiency, morbid leucorrhea, chronic infection and hematopoietic disorders (2). Extensive studies have been conducted to investigate the biological and physiochemical properties of Cistanche, which revealed that it exhibited neuroprotective, anti-inflammatory, antioxidant and antiaging effects (3).…”

Section: Introductionmentioning

confidence: 99%

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Protective effects of acteoside against X-ray-induced damage in human skin fibroblasts

Yang

Yao

Liu

et al. 2015

Molecular Medicine Reports

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Abstract. To investigate the protective effects of acteoside against apoptosis induced by X-ray radiation in human skin fibroblasts (HSFs), the cells were divided into the following groups: Control group; X-ray radiation group; acteoside group, in which the confluent cells were preincubated with 50 µg/ml acteoside for 2 h followed by radiation; and positive control group, in which the cells were preincubated with 50 µg/ml paeoniflorin followed by radiation. For the radiation, HSF cells preincubated with acteoside or paeoniflorin were exposed to X-ray beams at a dose-rate of 3 Gy/min (16 Gy in total). Cell viability, apoptosis and intracellular alteration of redox were monitored by MTT and flow cytometry. Compared with the radiation group, the number of cells arrested at the G0/G1 phase was significantly reduced in the acteoside and paeoniflorin groups, respectively (P<0.05). X-ray radiation induced marked apoptosis in HSF cells and acteoside reversed this effect. Compared with the radiation group, the generation of intracellular reactive oxygen species (ROS) was abrogated by pre-incubation with acteoside or paeoniflorin (P<0.05). In addition, the upregulation of pro-caspase-3 induced by radiation was reversed by acteoside or paeoniflorin. Radiation could induce upregulation of Bax and downregulation of Bcl-2; however, it was reversed completely after administration of acteoside or paeoniflorin. Furthermore, the enhanced expression of ERK and JNK induced by radiation was reversed by acteoside or paeoniflorin. Acteoside could protect the cells from X-ray induced damage through enhancing the scavenging activity of ROS, decreasing the Bax/Bcl-2 ratio and downregulating the activity of procaspase-3, as well as modulating the mitogen-activated protein kinase signaling pathways.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective effects of acteoside against X-ray-induced damage in human skin fibroblasts

Yang

Yao

Liu

et al. 2015

Molecular Medicine Reports

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“…Its clinical benefit has been reported in the treatment of many health problems including chronic constipation (2,3) and its beneficial effects are associated with its laxative activity in the intestine; it enhances moistening the stool, promoting bowel movement in patients (4,5), as well as in animal models (6,7). Our recent study demonstrates that oral administration of a water-extract of C. deserticola significantly reduces the number of inflammatory hyperplasia in the gut of colorectal cancer-prone mice (8).…”

Section: Introductionmentioning

confidence: 99%

Echinacoside Stimulates Cell Proliferation and Prevents Cell Apoptosis in Intestinal Epithelial MODE-K Cells by Up-Regulation of Transforming Growth Factor-β1 Expression

et al. 2012

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Abstract. Cistanche deserticola MA (C. deserticola) has been widely used as a laxative herbal in herbal medicine for the treatment of irritable bowel syndrome or constipation, and echinacoside (ECH) is one of the major bioactive ingredients in this herbal. Our aim was to investigate the effect of ECH on intestinal epithelial cell growth and death. MODE-K, an intestinal epithelial cell line, was used as an in vitro model of the intestine. Cell proliferation was measured by methylthiazol tetrazolium (MTT) assay. Cell apoptosis was determined with Annexin-V staining. Here we showed that in cultured MODE-K cells, ECH significantly stimulated cell proliferation and enhanced cell survival by reducing cell apoptosis in the presence of H 2 O 2 or the mixture of pro-inflammatory cytokines, while transforming growth factor (TGF)-β1 expression was up-regulated in a dose-dependent manner. Knockdown of TGF-β1 expression disrupted both the proliferative and cytoprotective activities of ECH, which was further confirmed by neutralization of TGF-β1 activity using anti-TGF-β1 antibody. These data suggest that ECH as one of bioactive ingredients in herbal C. deserticola and others may improve mucosal tissue repair by stimulating intestinal epithelial cell proliferation and preventing cell death via up-regulation of TGF-β.

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“…1) Pharmacological research has demonstrated that phenylethanoid glycosides and saccharides extracted from C. deserticola are the main active components. 2,3) Phenylethanoid glycosides (acteoside, echinacoside, 2′-acetylacteoside, cistanoside A, isoacteoside, etc.) mainly exhibit antioxidative, 4) neuroprotective, 5,6) and hepatoprotective 7) effects, while polysaccharides and soluble sugars notably show antioxidative, 8) immunological, [9][10][11] laxative, 12) and anti-aging activity.…”

mentioning

confidence: 99%

Effect of the Steaming Process on Quality of Postharvest <i>Cistanche deserticola</i> for Medicinal Use during Sun Drying

Peng

Wang

et al. 2016

Biological & Pharmaceutical Bulletin

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Effect of steaming on postharvest stems of Cistanche deserticola Y. C. MA was evaluated periodically during sun drying in both years. Steamed stems exhibited oily black in color, slightly heavier and longer than untreated ones. The levels of five phenylethanoid glycosides and 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging activity in steamed stems remained relatively stable during sun drying. Steamed samples showed higher amounts of water-soluble extracts, dilute ethanol-soluble extracts, soluble sugars, and polysaccharides, while a lower level of total ashes than untreated samples. The overall results suggest that steaming is an effective processing for enhancing the appearance quality and the concentration of some bioactive compounds in Cistanches Herba.

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Analysis of chemical constituents in Cistanche species

Cited by 144 publications

References 37 publications

Protective effects of acteoside against X-ray-induced damage in human skin fibroblasts

Protective effects of acteoside against X-ray-induced damage in human skin fibroblasts

Echinacoside Stimulates Cell Proliferation and Prevents Cell Apoptosis in Intestinal Epithelial MODE-K Cells by Up-Regulation of Transforming Growth Factor-β1 Expression

Effect of the Steaming Process on Quality of Postharvest <i>Cistanche deserticola</i> for Medicinal Use during Sun Drying

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