Analysis of chloramphenicol residues in swine tissues and milk: comparative study using different screening and quantitative methods

Water, C. van de; Haagsma, N.

doi:10.1016/0378-4347(91)80122-s

Cited by 26 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A common approach to extract CAP from food entails the use of a simple extraction step with organic solvents (Crechido and Hill 1979, Bories et al 1983, Allen 1985, Keukens et al 1986, Van De Water and Haagsma 1991, Ramos et al 1994. However, metabolism of CAP into the glucuronide adduct has been observed in liver, and several authors have reported the use of enzymatic digestion with b-glucuronidase before extraction in order to liberate conjugated CAP (Cooper et al 1998).…”

Section: Analyte Extractionmentioning

confidence: 98%

“…Different analytical methods have been developed to determine CAP in food, including radio and enzyme immunoassay (Arnold and Somogyi 1985, Charm and Chi 1988, Sheth and Sporns 1990, Van De Water and Haagsma 1991, Lynas et al 1998, Kolosova et al 2000, Gaudin and Maris 2001, gas chromatography (GC) (Weber 1990, Pfenning et al 2000, highperformance liquid chromatography (HPLC) (Jurgens 1982, Keukens et al 1986, Long et al 1990, Smedley and Weber 1990, Samouris et al 1993, Ramos et al 1994, Chevalier et al 1995, Hummert et al 1995, Perez et al 2002 and negative chemical ionization gas chromatography coupled to mass spectrometry (GC-MS-NCI) (van der Heeft et al 1991, Kijak 1994.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Analysis of chloramphenicol in honeys of different geographical origin by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

Verzegnassi

Royer

Mottier

et al. 2003

Food Additives and Contaminants

View full text Add to dashboard Cite

A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to detect trace amounts of the antibiotic chloramphenicol (CAP) in honey. The methodology entailed a solid-phase extraction of aqueous honey solutions followed by liquid-liquid partitioning, filtration and direct injection onto the LC-MS/MS system. Honey extracts were spiked with an isotopically labelled internal standard (d(5)-CAP) to compensate for analyte loss and potential ion suppression during the MS stage. Detection of the analyte was achieved by negative ionization electrospray in the selected reaction monitoring (SAM) mode. For confirmation, four characteristic mass transitions were monitored each for the analyte and the surrogate standard. The method was validated according to the latest European Union criteria for the analyses of veterinary drug residues in food. At all three fortification levels studied (0.1, 0.2, 0.5 microg kg(-1)) the method was accurate to within 15%. The repeatability and within-laboratory reproducibilities were <12 and 18%, respectively. The decision limit (CC alpha) and detection capability (CC beta) were both <0.1 microg kg(-1). The procedure provides a sensitive and reliable method for the determination of residues of chloramphenicol in honey. Numerous raw honeys of various geographical origins were analysed, showing extensive contamination particularly those of Chinese origin.

show abstract

Section: Analyte Extractionmentioning

confidence: 98%

mentioning

confidence: 99%

Analysis of chloramphenicol in honeys of different geographical origin by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

Verzegnassi

Royer

Mottier

et al. 2003

Food Additives and Contaminants

View full text Add to dashboard Cite

show abstract

“…During the last decade immunoaffinity (IA) chromatography had proved its worth as a versatile tool for the efficient isolation of contaminants and residues from food and feed. [5][6][7] For the analysis of penicillins, however, this promising approach in analytical chemistry currently plays no role mainly due to the lack of appropriate antibodies. The only described approach for the preparation of a multi-analyte penicillin IA column failed due to the instability of the employed egg yolk antibodies.…”

Section: Introductionmentioning

confidence: 99%

The potential of monoclonal antibodies against ampicillin for the preparation of a multi-immunoaffinity chromatography for penicillins†

Dietrich¹,

Usleber²,

Märtlbauer³

1998

Analyst

View full text Add to dashboard Cite

Monoclonal antibodies (Mab) against ampicillin were prepared by immunization of mice with an ampicillin-keyhole limpet hemocyanin conjugate coupled by a glutaraldehyde method. Sensitivity and specificity of these antibodies were tested in a direct competitive enzyme immunoassay, in which an ampicillin-horseradish peroxidase conjugate prepared by a carbodiimide method served as the labelled antigen. According to their cross-reactivities with the other beta-lactam antibiotics, the Mabs could be divided into two groups, which are represented by the clones designated 1D1 and 3B5. While Mab 3B5 (IgG1) showed no major cross-reactions with the other penicillins frequently used in veterinary medicine except for amoxicillin (108%), Mab 1D1 (IgG2a) had marked cross-reactivities with most of the 17 tested beta-lactam antibiotics (e.g., amoxicillin 187%, penicillin G 31%, cloxacillin 30%, dicloxacillin 44%, and oxacillin 14%). The detection limits for ampicillin, calculated from the antibiotic concentration giving 30% binding inhibition, were 11.7 (Mab 3B5) and 16.6 ng ml-1 (Mab 1D1). To prepare multi-immunoaffinity chromatography columns, Mab 1D1 and a previously described antibody against cloxacillin (Mab 1F7) were each coupled to CNBr activated sepharose. The capacity of the resulting immunosorbents was approximately 6.6 and 5.4 micrograms ml-1 gel for ampicillin and cloxacillin, respectively. Recoveries of amoxicillin, ampicillin, cloxacillin, dicloxacillin, penicillin G and oxacillin (in buffer solutions) from the produced immunoaffinity columns were in the range from 67 to 100%.

show abstract

“…Several techniques are used for the determination of CAP residues in foods, such as enzyme‐linked immunosorbent assay (ELISA),20, 21 radioimmunoassay,22 LC/MS/MS,23–29 and gas chromatography/mass spectrometry (GC/MS),30, 31 both as screening and confirmatory methods.…”

mentioning

confidence: 99%

In‐house validation of a liquid chromatography/electrospray tandem mass spectrometry method for confirmation of chloramphenicol residues in muscle according to Decision 2002/657/EC

Vinci

Guadagnuolo

Danese

et al. 2005

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

In this work we present an in-house validation study for the confirmatory analysis of chloramphenicol (CAP) in muscle according to the Commission Decision 2002/657/EC requirements. CAP is extracted in acetonitrile and after liquid-liquid partitioning with n-hexane is identified and quantitatively determined by ion trap liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI-MS/MS) analysis in the negative ion mode. CAP was identified using the precursor ion and at least two product ions, meeting the qualitative and quantitative criteria set by the European Commission in the Decision 2002/657/EC for confirmation of prohibited veterinary drug residues. We calculated mean drug recoveries, CCalpha and CCbeta of the method, and reported data on specificity, ruggedness and within-laboratory reproducibility. Finally, we point out and discuss some problems and questions arising from controversy about the application of Decision 2002/657/EC.

show abstract

Analysis of chloramphenicol residues in swine tissues and milk: comparative study using different screening and quantitative methods

Cited by 26 publications

References 8 publications

Analysis of chloramphenicol in honeys of different geographical origin by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

Analysis of chloramphenicol in honeys of different geographical origin by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

The potential of monoclonal antibodies against ampicillin for the preparation of a multi-immunoaffinity chromatography for penicillins†

In‐house validation of a liquid chromatography/electrospray tandem mass spectrometry method for confirmation of chloramphenicol residues in muscle according to Decision 2002/657/EC

Contact Info

Product

Resources

About