Analysis of chlorophyll a fluorescence and proteomic differences of rice leaves in response to photooxidation

Ma, Jing; Lv, Chunfang; Xu, Minli; Hao, Peifei; Wang, Yuwen; Shen, Weijun; Gao, Zhengyuan; Chen, Guoxiang; Lv, Chuangen

doi:10.1007/s11738-016-2342-0

Cited by 10 publications

(4 citation statements)

References 46 publications

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“…Chl fluorescence has a close relationship with plant photosynthesis, and Chl fluorescence analysis has been proven to be a useful tool in monitoring the influence of various stress conditions, such as salt (Mehta et al, 2010; Sun et al, 2016), high temperature (Mathur et al, 2015), pH (Tongra et al, 2011; Long et al, 2017), drought (Oukarroum et al, 2007), fluoranthene (Rupal and Anjana, 2013), photooxidation (Jing et al, 2017), and heavy metals (Hendrik et al, 2010; Goussi et al, 2018), on the photosynthetic activity of plants or microalgae/cyanobacteria. Although Chl fluorescence emissions only account for 2–5% of the energy that is not used in photosynthesis (Zhao et al, 2018), it can supply extensive information related to the components,structure, and function of plant photosynthetic performance (Strasser et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Detection of Cadmium Risk to the Photosynthetic Performance of Hybrid Pennisetum

et al. 2019

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Photosynthesis plays an essential role in plant growth and crop yield, and the mechanisms of the effects of cadmium (Cd) on photosynthetic performance require more attention. The acute toxicity of Cd in soil to the photosynthetic capacity of Hybrid Pennisetum was evaluated using gas exchange parameters, A / C i curves, light response curves, and chlorophyll a fluorescence transients after exposure to elevated Cd concentrations (0, 10, 20, 50, 70, and 100 mg kg −1 ) for a 3-month period. The results indicated that leaf Cd concentration in Hybrid Pennisetum increased with the strength of soil Cd stress and ranged from 4.9 to 15.8 μg g −1 DW. The accumulation of leaf Cd severely restricted photosynthesis and its non-stomatal limitation in regulating the photosynthetic performance of Hybrid Pennisetum . The leaf chloroplasts at 10 and 20 mg kg −1 Cd concentrations showed no noticeable change, but the chlorophyll content significantly decreased by 9.0–20.4% at 50–100 mg kg −1 Cd concentrations. The Cd treatments also decreased plant ribulose-1,5-bisphosphate (RuBP) activity ( V cmax ) and regeneration capacity ( J max ), triose phosphate utilization ( TPU ), light-saturated photosynthesis ( A max ), apparent quantum yield ( AQY ), light saturation point ( LSP ), and dark respiration ( R day ), but Cd treatment increased the light compensation point ( LCP ). The shape of chlorophyll a fluorescence transients in leaves was altered under different Cd treatments. The increased OJ phase and the decreased IP phase in fluorescence induction curves suggested that Cd toxicity inhibited both light use efficiency and photodamage avoidance ability. These results suggested that the decrease in photosynthesis through exposure to Cd may be a result of the decrease in leaf chlorophyll content, Rubisco activity, and RuBP regeneration, inhibition of triose phosphate utilization, reduction of the ability to use light and provide energy, and restrictions on electron transport in PSII.

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Section: Discussionmentioning

confidence: 99%

Detection of Cadmium Risk to the Photosynthetic Performance of Hybrid Pennisetum

et al. 2019

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“… 85–87 FLBR allows O 2 respiration in the root nodules, protecting them from disease, and is a defense against biotic stresses. 88 , 89 Although the leaves in 4% FS had shaded stress in the absence of the Rubisco proteome, the roots of P. indicus seedlings in the low light may have stress tolerance by increasing FLBR. Glycine dehydrogenase is usually decreased under abiotic stresses, including chilling (cold) and heavy metal exposure, which results in low photorespiration efficiency in the leaves.…”

Section: Discussionmentioning

confidence: 99%

Seedling growth and photosynthetic response of Pterocarpus indicus L. to shading stress

Lee

Kim

Kantharaj

et al. 2023

Plant Signaling & Behavior

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In tropical forests, the shade provided by tree canopies and extreme climate causes inhibition of plant seedling growth due to the lack of light. However, the plants can acclimate to such environmental stress by generating specific responses. The present study aimed to investigate the effects of shading conditions on ecophysiological performance of Narra seedlings ( Pterocarpus indicus L.) via a mesocosm experiment. A pot experiment was conducted for 20 weeks in a greenhouse with different shading treatments, 75% (control), 25%, and 4% of full sunlight (FS). As a result, the photosynthetic rate ( P N ), Rubisco enzyme activity, maximum carboxylation rate ( V Cmax ), and maximum electron transport rate ( J max ) in 25% FS treatment were higher or similar to those in control after three weeks of the beginning of shade treatment, whereas the highest values after ten weeks were observed in control. In contrast, the photosynthetic pigments were highest in control after three weeks, while the values were highest in 25% FS treatment after ten weeks. The growth parameters, such as biomass and leaf area, were highest in 75% FS treatment. The expression of Rubisco, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and fructose-1,6-bisphosphatase were up-regulated in 4% FS treatment compared to control after ten weeks, contributing to tolerating the shade stress. Our findings indicated the capacity of P. indicus seedlings to tolerate and acclimate low light conditions causing shade stress by generating specific physiological and morphological responses, especially Rubisco enzyme activity as well as gene expression related to photosynthetic activity. The present study will improve our understanding of the tolerance mechanism of Narra plant under light-deficient conditions, thereby providing a better strategy for efficiently growing seedlings of this species in tropical rainforests.

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“…This suggested a strategy of photo-protection for prevention over a reduction in rice electron transport chain [32]. In the interim, it can endorse the release of energy surplus and curtail the photooxidative injury in rice plants [33].…”

Section: Psii Efficiency and Excitation Energy Dissipationmentioning

confidence: 99%

Comparative Physiological, Biochemical, and Proteomic Responses of Photooxidation-Prone Rice Mutant 812HS under High Light Conditions

et al. 2021

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Photosynthetic efficiency decreases as light energy surpasses the photosynthesis capacity. This study was designed to investigate the potential effects of high-intensity light on the photooxidation-prone mutant 812HS of rice and its wild-type 812S during yellow and recovering stages. Results showed that in the yellowing stage, light oxidation occurs due to the exposure of mutant 812HS leaves to the high sunlight, which causes yellowing of the leaves, leading to a reduction in the photochemical activities, physiological mechanisms, and protein contents in mutant 812HS. In the recovery stage, mutant 812HS leaves were exposed to the maximum high brightness, the mutant’s leaves were draped with a dark cover to decrease the exposure of leaves of the plants from direct sunlight, which leads to the restoration of the green color again to the mutant 812HS leaves, leading to improving the performance of the photochemical activities, physiological mechanisms, and protein contents in mutant 812HS. Exposing leaves of mutant 812HS to high light at the yellow stage also resulted in a decrease in the net photosynthetic rate (Pn) in carotenoids content and chlorophyll a and b. Similarly, chlorophyll fluorescence of mutant 812HS decreased in (O-I-J-I-P) curves, and the ATP content, Mg2+-ATPase, and Ca2+-ATPase activities also decreased. An increase in energy dissipation was observed, while ABS/RC, DI0/RC, and TR0/RC values in mutant 812HS at the yellow stage increased. During photooxidation, an increase in O2•– and H2O2 contents was observed in mutant 812HS. While O2•– and H2O2 contents were decreased in mutant 812HS at the recovery stage. The rate of thylakoid membrane protein content was significantly decreased in mutant 812HS at the yellow stage, while at the recovery stage, there was no significant decrease. Our findings showed that photooxidation prompted oxidative damages and lipid peroxidation that caused severe damages to the membranes of the cell, photosynthetic pigments degradation, protein levels, and photosynthesis inhibition in mutant 812HS.

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Analysis of chlorophyll a fluorescence and proteomic differences of rice leaves in response to photooxidation

Cited by 10 publications

References 46 publications

Detection of Cadmium Risk to the Photosynthetic Performance of Hybrid Pennisetum

Detection of Cadmium Risk to the Photosynthetic Performance of Hybrid Pennisetum

Seedling growth and photosynthetic response of Pterocarpus indicus L. to shading stress

Comparative Physiological, Biochemical, and Proteomic Responses of Photooxidation-Prone Rice Mutant 812HS under High Light Conditions

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