Analysis of ciliary beat frequency and ovum transport ability in the mouse oviduct

Shi, Dongbo; Komatsu, Kouji; Uemura, Tadashi; Fujimori, Toshihiko

doi:10.1111/j.1365-2443.2011.01484.x

Cited by 40 publications

(41 citation statements)

References 24 publications

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“…Although CBF may not be equated with fluid movement itself [Shi et al, 2011], CBF is usually considered to be a valuable indicator and, therefore, is used as one criterion in the functional assessment of primary ciliary dyskinesia [Mantovani et al, 2010]. In our tubal samples, mean CBF was 11.9 ± 4.1 Hz at 36 ± 0.2 ° C. This is in good agreement with values reported for tracheal and tubal cilia in various species [Joki and Saano, 1994;Ryser et al, 2007;Shi et al, 2011], yet at some variance from a CBF of 5.3 ± 0.2 Hz that was reported for human tubal epithelium [Lyons et al, 2002]; in this study, however, epithelial temperature was not taken into account. As is known, this factor has a high impact on CBF [Ryser, 2009] and thus provides a plausible explanation for this difference.…”

Section: Discussionmentioning

confidence: 99%

“…Rather, investigations on tubal cilia have focused on the incidence of structural anomalies in healthy and diseased subjects [Roperto et al, 1990[Roperto et al, , 1991a[Roperto et al, , 1991b[Roperto et al, , 1995[Roperto et al, , 1996[Roperto et al, , 1998] and on CBF [Lyons et al, 2002;Kölle et al, 2009;Shi et al, 2011;Noreikat et al, 2012] but have disregarded parameters such as the coherence in axonemal orientation or the correlation between the plane of the active stroke and the direction of the mucociliary transport. Although current knowledge emphasizes the importance of ciliary motility in female fertility, neither the orientation of oviductal cilia nor its effects on directed tubal transport have been investigated to date.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ciliary Beating Plane and Wave Propagation in the Bovine Oviduct

Schätz

Schneiter²,

Rička³

et al. 2013

Cells Tissues Organs

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The uterine tube is an essential conduit for the gametes and zygote during reproduction. The necessary bidirectional conveyance occurs through peristalsis and ciliary activity, but unlike in respiratory tract, little is known about mucociliary transport in the uterine tube, and the direction of transport and the alignment of oviductal cilia have not been conclusively characterized. This study aimed to determine the uniformity in the axonemal orientation of motile cilia in the bovine uterine tube, to identify the direction of mucociliary transport and to relate the presumptive beating plane and the mucociliary transport direction to the long axis of the uterine tube. The angular spread of oviductal motile cilia was determined by electron microscopy, and by maintaining the accurate alignment of the samples throughout the processing steps, axonemal orientation was determined relative to the long axis of the oviduct. The direction of the effective mucociliary transport was determined by the analysis of video microscopic data recorded on explants. Vector-based analysis of electron micrographs yielded the mean angle of deviation between the ‘effective ciliary stroke', as derived from axonemal orientation, and the tubal longitudinal axis pointing towards the uterus to be 0.8°, with a standard deviation of 35.2°. The corresponding angular deviation of the short-wave propagation was -6.8° (SD 34.6°). These results show that oviductal motile cilia are rigorously aligned, that the beating plane of the cilia is parallel to the long axis of the uterine tube and that the ‘effective stroke' and mucociliary transport are directed towards the uterus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ciliary Beating Plane and Wave Propagation in the Bovine Oviduct

Schätz

Schneiter²,

Rička³

et al. 2013

Cells Tissues Organs

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“…About 80 percent of epithelial cells are ciliated in the infundibulum (Agduhr, 1927;Stewart and Behringer, 2012;Yamanouchi et al, 2010), which is the region close to the open end of the oviduct. Although the myosalpinx contractions also play roles in the transportation of ova, the ciliary movements at the infundibulum are sufficient to generate oviductal flow and to carry the oocytes towards the uterus (Halbert et al, 1989;Halbert et al, 1976;Shi et al, 2011). The orchestrated direction of ciliary motion is thought to be a key to ensure oocyte transport.…”

Section: Introductionmentioning

confidence: 99%

Celsr1 is required for the generation of polarity at multiple levels of the mouse oviduct

et al. 2014

Self Cite

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The oviduct is an important organ in reproduction where fertilization occurs, and through which the fertilized eggs are carried to the uterus in mammals. This organ is highly polarized, where the epithelium forms longitudinal folds along the ovary-uterus axis, and the epithelial multicilia beat towards the uterus to transport the ovulated ova. Here, we analyzed the postnatal development of mouse oviduct and report that multilevel polarities of the oviduct are regulated by a planar cell polarity (PCP) gene, Celsr1. In the epithelium, Celsr1 is concentrated in the specific cellular boundaries perpendicular to the ovary-uterus axis from postnatal day 2. We found a new feature of cellular polarity in the oviduct -the apical surface of epithelial cells is elongated along the ovary-uterus axis. In Celsr1-deficient mice, the ciliary motion is not orchestrated along the ovary-uterus axis and the transport ability of beating cilia is impaired. Epithelial cells show less elongation and randomized orientation, and epithelial folds show randomized directionality and ectopic branches in the mutant. Our mosaic analysis suggests that the geometry of epithelial cells is primarily regulated by Celsr1 and as a consequence the epithelial folds are aligned. Taken together, we reveal the characteristics of the multilevel polarity formation processes in the mouse oviduct epithelium and suggest a novel function of the PCP pathway for proper tissue morphogenesis.

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“…The beats of the cilia are coordinated and appear rhythmically at a local level, but have a range of frequencies along the entire tube. The ciliated cells of the infundibulum induce unidirectional flows resulting in the delivery of an ovum by their ciliary activities, although their beating periodicity is asynchronous (Shi et al, 2011). The early embryo seems to be capable of down-regulating the speed of transport at a local level, which increases its length of stay (Koelle et al, 2009).…”

Section: Physiological Events During Embryo Passage Through the Oviductmentioning

confidence: 99%

Dialogue between the preimplantation embryo and the oviduct

Besenfelder¹,

Havlíček²,

Brem³

2019

Proc

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Knowledge about early embryogenesis and the role of the oviduct has gained renewed interest due to increasing awareness of the impact of animal health status and performance on the susceptibility and vulnerability of early developing embryos within their microenvironment. The significance of the oviduct is best illustrated when a glance is cast at structural as well as functional features of the oviduct which regulate the serially orchestrated and well-tuned steps in early embryogenesis. A lot of work has been done in vitro to bypass the oviduct consequently resulting in steadily increasing information about the requirements of early developing embryos. However, relatively little information is available to demonstrate direct tubal effects on gametes and embryos. There is substantial evidence that the bovine oviduct provides a source of high quality embryos, our understanding of this interaction is far from complete. Allen WR, Stewart F, Trounson AO, Tischner M & BielanskiW 1976 Viability of horse embryos after storage and long-distance transport in the rabbit. J Reprod Fertil 47 387-390.

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Analysis of ciliary beat frequency and ovum transport ability in the mouse oviduct

Cited by 40 publications

References 24 publications

Ciliary Beating Plane and Wave Propagation in the Bovine Oviduct

Ciliary Beating Plane and Wave Propagation in the Bovine Oviduct

Celsr1 is required for the generation of polarity at multiple levels of the mouse oviduct

Dialogue between the preimplantation embryo and the oviduct

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