Analysis of coal tar pitch and smoke extract components and their cytotoxicity on human bronchial epithelial cells

Li, Zhitao; Wu, Yongjun; Zhao, Yong; Wang, Lixia; Zhu, Hansong; Qin, Lijuan; Feng, Feifei; Wang, Wei; Yi-ming, WU

doi:10.1016/j.jhazmat.2010.11.123

Cited by 45 publications

(14 citation statements)

References 17 publications

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“…Coal tar pitch (CTP), a by‐product in the smoking process, is a crude material used in paint, rubber, synthon, medication, and heat‐resistant materials . And it has been confirmed that CTP extract constitutes polycyclic aromatic hydrocarbons (87.91%) and the remaining (12.09%) including monocyclic aromatic hydrocarbons, heterocyclic compounds and alkenes using gas chromatography/mass spectrometry . Previous research demonstrated that CTP extract is involved in the development of lung cancer.…”

Section: Introductionmentioning

confidence: 99%

LncRNA‐ENST00000501520 promotes the proliferation of malignant‐transformed BEAS‐2B cells induced with coal tar pitch mediated by target genes

Zhang

Meng

et al. 2019

Environmental Toxicology

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As a human carcinogen, coal tar pitch (CTP) can significantly increase the risk of lung cancer. However, the mechanism underlying CTP‐induced lung carcinogenesis has not been well understood. This study aims to explore the role of the LncRNA‐ENST00000501520 in the proliferation of malignant‐transformed human bronchial epithelial cells (BAES‐2B) induced by CTP extract for the first time. BEAS‐2B cells were stimulated with 2.4 μg/mL CTP extract, and then passaged for three times, which were named passage 1 and then passaged until passage 30 (named as CTP group). The ENST000001520 of cells in CTP group was interfered using siRNA. The results showed that ENST000001520 located in cell nucleus (>80%) had no or weak ability of protein encoding. After interference of ENST000001520, the migration and proliferation of cells in CTP group were inhibited, and the cell cycle was arrested in the G0/G1 phase; however, the apoptosis of cells in CTP group was promoted. The target genes (SKB1, CLTB, TAP2, PIPK2, and SOCS3) of ENST000001520 were screened out, and the mRNA and protein expression of SBK1 and SOCS3 was significantly decreased after ENST000001520 interference. SBK1 and SOCS3 may play a promoting role in occurrence and development of cancers. The study suggests that LncRNA‐ENST00000501520 could promote the proliferation in malignant‐transformed BEAS‐2B cells induced with CTP extract which may be mediated by target genes. This study may provide a new target for prevention and treatment of lung cancer.

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Section: Introductionmentioning

confidence: 99%

LncRNA‐ENST00000501520 promotes the proliferation of malignant‐transformed BEAS‐2B cells induced with coal tar pitch mediated by target genes

Zhang

Meng

et al. 2019

Environmental Toxicology

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“…CTPE was collected as described previously [24]. Briefly, moderate-temperature CTP was grinded into powder in an agate bowl, sieved by 200 mesh sieves and heated at 400°C on the electric hot plate in a flow hood.…”

Section: Methodsmentioning

confidence: 99%

Macrophages Facilitate Coal Tar Pitch Extract-Induced Tumorigenic Transformation of Human Bronchial Epithelial Cells Mediated by NF-κB

et al. 2012

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ObjectiveChronic respiratory inflammation has been associated with lung cancer. Tumor-associated macrophages (TAMs) play a critical role in the formation of inflammation microenvironment. We sought to characterize the role of TAMs in coal tar pitch extract (CTPE)-induced tumorigenic transformation of human bronchial epithelial cells and the underlying mechanisms.MethodsThe expression of TAMs-specific CD68 in lung cancer tissues and paired adjacent tissues from cancer patients was determined using immunostaining. Co-culture of human bronchial epithelial cells (BEAS-2B) and macrophage-like THP-1 cells were conducted to evaluate the promotive effect of macrophages on CTPE-induced tumorigenic transformation of BEAS-2B cells. BEAS-2B cells were first treated with 2.4 µg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured either with or without THP-1 cells and passaged using trypsin-EDTA. Alterations of cell cycle, karyotype, colony formation in soft agar and tumor xenograft growth in nude mice of BEAS-2B cells at passages 10, 20 and 30, indicative of tumorigenecity, were determined, respectively. In addition, mRNA and protein levels of NF-κB in BEAS-2B cells were measured with RT-PCR and western blot, respectively. B(a)P was used as the positive control.ResultsThe over-expression of TAMs-specific CD68 around lung tumor tissues was detected and associated with lung cancer progression. The tumorigenic alterations of BEAS-2B cells including increase in cell growth rate, number of cells with aneuploidy, clonogenicity in soft agar, and tumor size in nude mice in vivo occurred at passage 10, becoming significant at passages 20 and 30 of the co-culture following CTPE removal in compared to BEAS-2B cells alone. In addition, the expression levels of NF-κB in BEAS-2B cells were positively correlated to the malignancy of BEAS-2B cells under different conditions of treatment.ConclusionThe presence of macrophages facilitated CTPE-induced tumorigenic transformation of BEAS-2B cells, which may be mediated by NF-κB.

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“…However, when petroleum pitches and coal tar pitches are carbonized, the reactant dehydrogenation polymerization, aromatization and rearrangement process [1] can release volatiles that may cause cancer at high doses. These can include polycyclic aromatic hydrocarbons (PAHs) in the range of 3-7 rings, and heterocyclic compounds [2][3]. Moreover, these pitches are comprised of PAHs; and the European REACH regulations are imposing strict protocols for transporting and processing PAHs.…”

Section: Introductionmentioning

confidence: 99%

Lignin/collagen hybrid biomaterials as binder substitute for specialty graphites and electrodes

Zhao

Cannon

Nieto-Delgado

et al. 2016

Carbon

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Sustainable biomaterial binders were developed from lignin and collagen to replace the conventional petroleum pitch and coal tar pitch binders that have been used when making specialty graphites and graphite electrodes. The team prepared lab-scale graphite electrodes by first creating green composites, by hot-pressing together the lignin, collagen, petroleum coke, and other additives, followed by carbonization and graphitization. Response Surface Methodology (RSM) experimental design was employed, based on Box-Behnken Design (BBD).This was employed to optimize the recipes and processing protocols for the green composite hot-2 pressing of 3.5 cm diameter × 19 cm long cylinder specimens. Relative to density, the apparent optimum conditions occurred with 13.0% lignin, 3.6% collagen, 27.4% petroleum coke fines, and the balance of petroleum coke particles plus some additives. The green hot-pressing protocol employed 30 MPa pressure at 400 ℃ for 2 hours. Baking peaked at 800 ℃, ramped up and down over 13 days. This baked product hosted an apparent density of 1.67 g/mL, and exhibited 6.2% puffing. Another baked product hosted 1.56 g/mL and 1.67% puffing. After the baked product was graphitized, we achieved an apparent density of 1.51 g/mL and resistance of 25-30 µΩm.These results approached the specifications for specialty graphites.

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Analysis of coal tar pitch and smoke extract components and their cytotoxicity on human bronchial epithelial cells

Cited by 45 publications

References 17 publications

LncRNA‐ENST00000501520 promotes the proliferation of malignant‐transformed BEAS‐2B cells induced with coal tar pitch mediated by target genes

LncRNA‐ENST00000501520 promotes the proliferation of malignant‐transformed BEAS‐2B cells induced with coal tar pitch mediated by target genes

Macrophages Facilitate Coal Tar Pitch Extract-Induced Tumorigenic Transformation of Human Bronchial Epithelial Cells Mediated by NF-κB

Lignin/collagen hybrid biomaterials as binder substitute for specialty graphites and electrodes

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