Analysis of complex lipid mixtures by thin‐layer chromatography and complementary methods

Abstract

ConclusionFurther work has been done on the application of thin‐layer adsorption chromatography to the fractionation of complex lipid mixtures into classes.New methods, the use of siliconized silicic acid plates and the application of thin‐layer adsorption chromatography combined with the complementary techniques of gas‐liquid chromatography and paper chromatography, are presented for the resolution of classes of lipids into their constituents.In contrast to such elaborate conventional techniques as column chromatography, analyses using the methods reported in this paper can be performed rapidly in large numbers on a routine basis.

Myocardial Function and Lipid Metabolism in the Chronic Alcoholic Animal

“…A portion of the chloroform extract was evaporated in a liquid scintillation vial and used to determine total lipid radioactivity in the original sample. A second aliquot of the chloroform extract was concentrated for separation of lipid classes by thin-layer chromatography (37). Identified spots from all thin-layer plates were scraped into miniature funnels with the aid of suction and eluted with chloroform: methyl alcohol (2: 1) into liquid scintillation vials for assay of radioactivity.…”

Long-Term Feeding of Formulas High in Linolenic Acid and Marine Oil to Very Low Birth Weight Infants: Phospholipid Fatty Acids

“…In study 1, the total lipid extract was spotted on a 10 (wide) x 20 (high) cm plate. Triglycerides were separated from total phospholipids (24). The total phospholipids that remained at the origin were then separated as described above by a modification of the solvent system of Zail and Pickering (23).…”

Lipid Composition of a Green Alga,Botryococcus braunii

“…The alga has been proposed as a renewable source of fuels from recent concern for future energy requirements. It occurs throughout the world in a wide variety of climates and includes several strains which are classified into two races, A and B.ll The A race accumulates linear olefins, odd numbered from 23 to 31, chiefly CZ7 , CZ9 ' and C31 dienes or trienes, whereas the B race produces polyunsaturated triterpenes of the general formula CnH2n-10, n = 30 ~ 37, termed botryococcenes. 1 -4) These findings suggest that the same strain is unable to yield successively the two types of hydrocarbons contrary to previous proposals.…”