Analysis of differentially expressed proteins after EHP-infection and characterization of caspase 3 protein in the whiteleg shrimp (Litopenaeus vannamei)

Liu, Hongtao; Shen, Minghui; He, Yugui; Li, Bingshun; Pu, Liang; Xia, Guangyuan; Yang, Mingqiu; Wang, Guofu

doi:10.1016/j.fsi.2023.108698

Cited by 5 publications

(2 citation statements)

References 47 publications

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“…These genes include adenosine deaminase , c-type lectin [ 92 ], caspase , chitinase , eukaryotic translation initiation factor , myosin light chain , ras-like GTP-binding protein Rho , relish, and serine protease (SP), which play crucial roles in disease resistance mechanisms. Numerous studies have underscored their critical role in disease resistance mechanisms [ 93 , 94 , 95 , 96 , 97 , 98 ]. However, it is evident that these disease resistance-related gene markers have experienced a reduction in genetic polymorphism within the blue shrimp broodstock, likely due to recent potential inbreeding depression affecting disease resistance capabilities.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

Yang,

Chu,

Chen

et al. 2024

Animals

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The Pacific blue shrimp (Litopenaeus stylirostris) is a premium product in the international seafood market. However, intensified farming has increased disease incidence and reduced genetic diversity. In this study, we developed a transcriptome database for L. stylirostris and mined microsatellite markers to analyze their genetic diversity. Using the Illumina HiSeq 4000 platform, we identified 53,263 unigenes from muscle, hepatopancreas, the intestine, and lymphoid tissues. Microsatellite analysis identified 36,415 markers from 18,657 unigenes, predominantly dinucleotide repeats. Functional annotation highlighted key disease resistance pathways and enriched categories. The screening and PCR testing of 42 transcriptome-based and 58 literature-based markers identified 40 with successful amplification. The genotyping of 200 broodstock samples revealed that Na, Ho, He, PIC, and FIS values were 3, 0.54 ± 0.05, 0.43 ± 0.09, 0.41 ± 0.22, and 0.17 ± 0.27, respectively, indicating moderate genetic variability and significant inbreeding. Four universal microsatellite markers (CL1472.Contig13, CL517.Contig2, Unigene5692, and Unigene7147) were identified for precise diversity analysis in Pacific blue, Pacific white (Litopenaeus vannamei), and black tiger shrimps (Penaeus monodon). The transcriptome database supports the development of markers and functional gene analysis for selective breeding programs. Our findings underscore the need for an appropriate genetic management system to mitigate inbreeding depression, reduce disease susceptibility, and preserve genetic diversity in farmed shrimp populations.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

Yang,

Chu,

Chen

et al. 2024

Animals

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“…China's shrimp industry faces potential long-term challenges despite short-term yield success (Dong and Raghavan, 2022). Although the shrimp industry is widely researched, with many studies reporting on breeding, growth performance, genetics or genomic aspects and experimental conditions and/or observations (e.g., Li et al, 2021;Abdel-Latif et al, 2022;Liu et al, 2023;Huang et al, 2024), little is known on the current status, evolution and prospective of China's shrimp industry. Regarding both its marine and freshwater shrimp aquaculture, no abundant information was found that reviewed the trends in shrimp production over the last two decades.…”

Section: Introductionmentioning

confidence: 99%

Shrimp industry in China: overview of the trends in the production, imports and exports during the last two decades, challenges, and outlook

N’Souvi,

Sun,

Che

et al. 2024

Front. Sustain. Food Syst.

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China’s shrimp farming industry has experienced significant growth in recent years. With such a development, some major constraints linked to the industry’s sustainable development have emerged. This paper aims to present an overview of China’s shrimp industry over the last two decades, with special attention to farm-raised shrimp both in marine and inland waters. Documentary research on its recent evolution was carried out based on data collected from China Fishery Statistics Yearbooks 2003 to 2022 coupled with data from FAO. Findings indicated that there has been an upward trend in China’s total shrimp production over the last 20 years, with a growth rate of 120%, representing an average annual growth rate of 4.3%. The observed higher shrimp production was driven by expanded shrimp aquaculture production, with major species dominated by the Pacific whiteleg shrimp (litopenaeus vannamei), the black tiger shrimp (Penaeus monodon), the Chinese shrimp (Penaeus chinensis), and the Japanese prawn (Penaeus japonicus). Challenges associated with the intensification of the production and the driven environmental deterioration need to be properly addressed. First, the shrimp farming industry should be practiced while considering both environment and ecosystem protection. Applying such an organic farming principle presupposes some practices for addressing the existing issues threatening the sustainable growth of the industry. To that end, there is a need for innovative techniques that should be strengthened. Second, possible solutions to shrimp disease problems, namely prevention, early diagnosis, and various control techniques, should also be developed and improved, with an emphasis on the former. In addition, encouraging the use of good-quality feed in appropriate quantity and form is also of paramount importance. It is thus worth noting that further policies need to focus on promoting a large range of ecological shrimp aquaculture technologies that should be encouraged among farmers.

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The influence of various doses of Moringa oleifera extract on the antioxidant trait, cytokines, reproductive hormones performance, and gonadal histological profiles of Nile tilapia

Gad,

Emam,

Shourbela

et al. 2024

Aquacult Int

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Analysis of differentially expressed proteins after EHP-infection and characterization of caspase 3 protein in the whiteleg shrimp (Litopenaeus vannamei)

Cited by 5 publications

References 47 publications

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

Shrimp industry in China: overview of the trends in the production, imports and exports during the last two decades, challenges, and outlook

The influence of various doses of Moringa oleifera extract on the antioxidant trait, cytokines, reproductive hormones performance, and gonadal histological profiles of Nile tilapia

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