Analysis of extended-spectrum- -lactamase-producing Escherichia coli isolates collected in the GERM-Vet monitoring programme

Schink, Anne‐Kathrin; Kadlec, Kristina; Kaspar, Heike; Mankertz, Joachim; Schwarz, Štefan

doi:10.1093/jac/dkt123

Cited by 57 publications

(45 citation statements)

References 41 publications

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“…16,19,25,37 In this study, only CMY-2 (or SNP variant) was observed, which is also in line with the results observed in clinical samples of companion animals. 19,37 The majority of ESBLs observed here were of CTX-M-1 group and less commonly of CTX-M-9 group with only one TEM-52, a distribution also reported by others.…”

Section: Discussionsupporting

confidence: 91%

Characterization of ESBL- and AmpC-Producing Enterobacteriaceae from Diseased Companion Animals in Europe

BogaertsPierre

HuangTe-Din

BouchahroufWarda

et al. 2015

Microbial Drug Resistance

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The study aimed to characterize beta-lactam resistance mechanisms of Enterobacteriaceae isolates recovered from diseased dogs and cats between 2008 and 2010 in a European surveillance program (ComPath I) for the antibiotic susceptibility of bacterial pathogens. A total of 608 non-duplicated Enterobacteriaceae isolates were obtained prior antibiotic treatment from diseased dogs (n=464) and cats (n=144). Among the 608 Enterobacteriaceae isolates, 22 presented a minimal inhibitory concentration against cefotaxime above EUCAST breakpoints of susceptibility. All the 22 isolates remained susceptible to carbapenems. Ten isolates were confirmed as extended-spectrum-beta-lactamase (ESBL) producers by PCR-sequencing of bla coding genes including 9 blaCTX-M (CTX-M-1, 14, 15, 32,…) and 1 blaTEM-52 and 12 were AmpC-producing isolates (10 plasmidic CMY-2 group and 2 isolates overexpressing their chromosomal AmpC). ESBLs and plasmid-mediated AmpC (pAmpC)-producing isolates were mainly recovered from dogs (n=17) suffering from urinary tract infections (n=13) and originated from eight different countries. ESBL-bearing plasmids were mostly associated with IncFII incompatibility groups while CMY-2 was predominantly associated with plasmid of the IncI1 group. ESBL/pAmpC-producing Escherichia coli belonged to phylogroup A (n=5), B2 (n=4), and D (n=5). Multilocus sequence typing analysis revealed that among three CTX-M-15-producing E. coli, two belong to sequence type (ST) 131 and one to ST405. The presence of CTX-M-15 including on IncFII plasmids in E. coli ST131-B2 has also been described in isolates of human origin. This suggests the possibility of exchanges of these isolates from humans to companion animals or vice-versa.

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Section: Discussionsupporting

confidence: 91%

Characterization of ESBL- and AmpC-Producing Enterobacteriaceae from Diseased Companion Animals in Europe

BogaertsPierre

HuangTe-Din

BouchahroufWarda

et al. 2015

Microbial Drug Resistance

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“…The three ESBL genes detected at the farms are the most commonly observed genes on poultry meat in the Netherlands and are, with the exception of bla SHV-12 , equally common in humans (15 those observed in the current study (15,16,(36)(37)(38)(39)(40) In conventionally kept broilers, the prevalence of ESBL-producing E. coli is very high, with 100% of farms positive (37), and 77 to 94% of all retail chicken meat is contaminated with ESBL-producing E. coli bacteria (15,16). The prevalence in laying hens has not been published yet, but preliminary data suggest 100% positivity in conventionally kept laying hens, as well, albeit at lower concentrations than in broilers (unpublished observations).…”

Section: Discussionmentioning

confidence: 53%

Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli on Flies at Poultry Farms

Blaak

Hamidjaja

Hoek

et al. 2014

Appl Environ Microbiol

108

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In the Netherlands, extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli bacteria are highly prevalent in poultry, and chicken meat has been implicated as a source of ESBL-producing E. coli present in the human population. The current study describes the isolation of ESBL-producing E. coli from house flies and blow flies caught at two poultry farms, offering a potential alternative route of transmission of ESBL-producing E. coli from poultry to humans. Overall, 87 flies were analyzed in 19 pools. ESBL-producing E. coli bacteria were detected in two fly pools (10.5%): a pool of three blow flies from a broiler farm and a pool of eight house flies from a laying-hen farm. From each positive fly pool, six isolates were characterized and compared with isolates obtained from manure (n ‫؍‬ 53) sampled at both farms and rinse water (n ‫؍‬ 10) from the broiler farm. Among six fly isolates from the broiler farm, four different types were detected with respect to phylogenetic group, sequence type (ST), and ESBL genotype: A 0 /ST3519/SHV-12, A 1 /ST10/SHV-12, A 1 /ST58/SHV-12, and B1/ST448/CTX-M-1. These types, as well as six additional types, were also present in manure and/or rinse water at the same farm. At the laying-hen farm, all fly and manure isolates were identical, carrying bla TEM-52 in an A 1 /ST48 genetic background. The data imply that flies acquire ESBL-producing E. coli at poultry farms, warranting further evaluation of the contribution of flies to dissemination of ESBL-producing E. coli in the community. Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are increasing in prevalence worldwide (1, 2). ESBLs confer resistance to most beta-lactam antibiotics, including 3rd-and 4th-generation cephalosporins, which severely limits treatment possibilities for infections caused by these bacteria. Often, options for treatment are further restricted by the multiresistant nature of ESBL-producing bacteria, which has led to increased use of, and increasing prevalence of Enterobacteriaceae resistant to, last-resort antibiotics, such as carbapenems (3). Although initially ESBL production was mainly observed in hospital infections caused by Klebsiella pneumoniae, today it is also frequently associated with community-acquired infections, mostly urinary tract infections caused by Escherichia coli (4, 5), as well as commensal E. coli strains isolated from humans and food-producing animals (6-8).Not only can dissemination of ESBL-producing E. coli in the community be facilitated by direct contact with human or animal carriers, but also, the presence of the bacterium in environmental compartments, such as surface water (9-12) and wildlife (13), suggests that the environment should also be considered in this regard. In the Netherlands, ESBL-producing E. coli is highly prevalent in poultry: in 2009, ESBL-producing (and/or AmpC-producing) E. coli bacteria were detected on 100% (n ϭ 26) of Dutch broiler farms studied (14). Because of the high prevalence of ESBL-producing E. coli on Dutch retail chick...

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“…The latter two ST types are frequently identified as ESBL-carrying E. coli isolates not only in humans, but also in veterinary fields [32,33,36,37,38]. Notably, of 5 D1-O1-ST648 strains in 212 strains examined in the present study, 3 strains harbored both aac(3)-II and CTX-M-type ESBL genes (table 3), and another D1-O1-ST648 strain harbored the aac(6')-Ib-cr gene and was resistant to AMK.…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of Cross-Resistance to Aminoglycosides in Fluoroquinolone-Resistant Escherichia coli Clinical Isolates

Tsukamoto¹,

Ohkoshi²,

Okubo³

et al. 2013

Chemotherapy

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Background: Multidrug-resistant Escherichia coli, especially a lineage of O25b:H4-ST131, has increased and spread worldwide. The surveillance of cross-resistance of E. coli is necessary. Methods: Cross-resistance to fluoroquinolones (FQs) and aminoglycosides (AGs) was examined in E. coli isolated in Hokkaido Prefecture, Japan, between 2008 and 2009. Results: Gentamicin (GEN) resistance was more common in FQ-resistant isolates (30/112 strains; 26.8%) than in FQ-susceptible isolates (2/100 strains; 2%). The frequency of GEN resistance was similar in two groups of FQ-resistant strains, O25b:H4-ST131 genotype (22/87 strains; 25.3%) and a group of other FQ-resistant genotypes (8/25 strains; 32.0%). The main AG resistance gene was aac(3)-II (87.5% of GEN-resistant strains). The only amikacin-resistant strain which was FQ resistant carried the aac(6')-Ib-cr gene. CTX-M type extended-spectrum β-lactamase (ESBL) genes were also found in FQ-resistant strains at a high frequency. However, the number of strains with both ESBL and AG-modifying enzyme genes was relatively low (8 strains). Conclusion: All FQ-resistant strains, not only O25b:H4-ST131, appeared to preferentially acquire ESBL genes and/or genes encoding AG-modifying enzymes; however, the acquisitions of these genes seemed to occur independently.

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Analysis of extended-spectrum- -lactamase-producing Escherichia coli isolates collected in the GERM-Vet monitoring programme

Cited by 57 publications

References 41 publications

Characterization of ESBL- and AmpC-Producing Enterobacteriaceae from Diseased Companion Animals in Europe

Characterization of ESBL- and AmpC-Producing Enterobacteriaceae from Diseased Companion Animals in Europe

Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli on Flies at Poultry Farms

High Prevalence of Cross-Resistance to Aminoglycosides in Fluoroquinolone-Resistant Escherichia coli Clinical Isolates

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