Analysis of genetic diversity of four quails by microsatellite markers

Abstract

Genetic diversity of four quail populations, including Korean quails (maroon quails), Peking white quails, Chinese yellow quails and Chinese black quails, were analyzed by microsatellite markers, aiming to provide scientific basis for new breeds of Chinese black quails for egg production as well as the assessment, protection and utilization of Chinese quail’s genetic resources. The results showed that 48 alleles were detected by nine microsatellite markers in the four quail populations, with the mean of 5.33 alleles in each locus. The average effective number of alleles marked by the nine microsatellite markers in Chinese black quails, Peking white quails, Chinese yellow quails and maroon quails were 3.5338, 3.6135, 4.0312 and 3.6508 respectively. The average heterozygosity of the four quail populations were 0.6952, 0.7046, 0.7353 and 0.7096 respectively. The average polymorphic information content of nine microsatellite loci in four quail populations were 0.6204, 0.6587, 0.6942 and 0.6639, respectively, all of which were greater than 0.5, indicating the four populations’ copious genetic diversity. In this study, the average genetic differentiation coefficient among populations was 0.0349, so the genetic variation among populations accounted for 3.49%, which demonstrated that genetic variation among populations was just a small proportion of the total population genetic variation, and there was little differentiation among the four populations. Cluster analysis indicated that Chinese black quails and Peking white quails were firstly clustered, and then Chinese yellow quails and maroon quails were clustered, and finally the two were clustered together.

Sinai and Norfa chicken diversity revealed by microsatellite markers

“…The 20 microsatellite loci were all highly polymorphic and can be used as effective markers of local Egyptian chicken strains to analyze genetic diversity. Genetically, a microsatellite marker with a PIC>0.7 is considered as an ideal one for genetic studies (Bai et al, 2016). In the present study, some of the selected 20 microsatellite loci showed PIC values of over 0.7, reflecting the possibility of using these loci as candidate genes for the future genetic studies of local Egyptian chicken breeds.…”

Microsatellite DNA Markers and Their Correlation with Body Traits in Landrace, Duroc and Henan Western Black Pigs

“…Genetic diversity data were analyzed with POPGENE32 (Guo and Elston, 1999;Kavitha et al 2015;Jiménez-Mena et al 2016). Polymorphism information content value (PIC) for all the loci were estimated according to Deshmukh et al (2015) and Bai et al (2016b).…”

Correlation Analysis between MyoG Gene Polymorphism and Carcass Characteristics of Egg quails

“…At present, quail breeding is becoming more and more popular in poultry and quail is smaller than other poultry, so quail can be used as a good new experimental animal. Quail can be used as a research animal in many subjects such as poultry reproduction, histology, nutrition, endocrinology, embryology, physiology, pharmacology etc (Zhang et al, 2013;Li et al, 2019, Bai et al, 2016a, 2016b.The experimental values of quail in teaching and scientific studies are increasing gradually (Bai et al,2016c(Bai et al, , 2016d(Bai et al, , 2017. Few studies on correlation analysis between MyoG and carcass characteristics of quail are available.…”