Analysis of Genetic Variation of Fat Tailed-sheep in Southern Region of Jordan

Al-Atiyat, Raed M.; Tabbaa, M. J.; Salameh, Naser M.; Tarawneh, Khaled A.; Al-Shmayla, L.; Al-Tamimie, H.J.

doi:10.3923/ajava.2012.376.389

Cited by 11 publications

(4 citation statements)

References 21 publications

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“…Similar results were reported earlier in which the genetic phylogeny of Awassi sheep population was high when compared to different world sheep (Arranz et al, 2001). Furthermore, an example of a phylogenetic tree study among some geographical Awassi sheep ecotypes was also reported (Al-Atiyat et al, 2012). On the basis of microsatellite data, considerable genetic differentiation was recently reported in Saudi sheep (Musthafa et al, 2012).…”

Section: Phylogeny Tree Reconstructionsupporting

confidence: 85%

“…Such situation was likely due to sharing common sequences with less substitution rates from previous ancestors. For example, Al-Atiyat et al (2012) reported that Jordan fat-tailed Awassi sheep populations of southern parts were genetically closer to each other according to the neighborjoining method. Furthermore, it was shown that the neighbor-joining method was the best to infer evolutionary history of many species, such as clustering horseshoe crabs into seven clades (Ismail and Sarijan, 2011).…”

Section: Phylogeny Tree Reconstructionmentioning

confidence: 99%

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Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Al-Atiyat¹,

Aljumaah²

2014

Genet. Mol. Res.

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ABSTRACT. This study aimed to estimate evolutionary distances and to reconstruct phylogeny trees between different Awassi sheep populations. Thirty-two sheep individuals from three different geographical areas of Jordan and the Kingdom of Saudi Arabia (KSA) were randomly sampled. DNA was extracted from the tissue samples and sequenced using the T7 promoter universal primer. Different phylogenetic trees were reconstructed from 0.64-kb DNA sequences using the MEGA software with the best general time reverse distance model. Three methods of distance estimation were then used. The maximum composite likelihood test was considered for reconstructing maximum likelihood, neighbor-joining and UPGMA trees. The maximum likelihood tree indicated three major clusters separated by cytosine (C) and thymine (T). The greatest distance was shown between the South sheep and North sheep. On the other hand, the KSA sheep as an outgroup showed shorter evolutionary distance to the North sheep population than to the others. The neighbor-joining and UPGMA

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Section: Phylogeny Tree Reconstructionsupporting

confidence: 85%

Section: Phylogeny Tree Reconstructionmentioning

confidence: 99%

Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Al-Atiyat¹,

Aljumaah²

2014

Genet. Mol. Res.

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“…These results indicate high genetic variability among studied breeds. Slightly lower Ho values were reported previously in Awassi sheep [ 15 ]. It was suggested that higher Ho or He values are highly correlated with higher values of PIC [ 15 ].…”

Section: Discussioncontrasting

confidence: 47%

Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers

Jawasreh¹,

Ababneh²,

Ismail³

et al. 2018

Vet World

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Aim:This study was conducted to study the genetic and population structure of local (Awassi) and exotic (Romanov, Charollais, Assaf, Awassi, and Suffolk) sheep breeds in Jordan using eight microsatellite markers.Materials and Methods:A total of 125 sheep were used (25 from each breed) in the study. The number of alleles (A), the mean values of observed (Ho) and expected (He) heterozygosity, polymorphism information content (PIC), fixation index as a measure of heterozygote deficiency or excess, and Hardy–Weinberg equilibrium (HWE) were analyzed using PopGen and CERVUS softwares. Nei’s standard genetic distances among breeds and dendrogram of unweighted pair group method with arithmetic mean (UPGMA) were calculated and constructed using PopGen software.Results:A total of 40 alleles were detected with an average number of alleles of 5. The mean Ho value was higher than the mean He value for all breeds. Awassi breed showed the highest average PIC value while Romanov had the lowest. There was a significant (p<0.05) deviation from HWE at each locus within and between breeds. Deviations from HWE were found to be highly significant for all markers except OARFCP304 locus. The genetic distance estimates revealed a close relationship between Romanov and Charollais and between Awassi and Charollais. In the UPGMA dendrogram, Charollais, Romanov, and Awassi breeds were placed together in one main cluster while Assaf was in a different subcluster. Awassi was placed alone in a second main cluster.Conclusion:Results of this study offer insight toward the genetic conservation of the studied breeds and a base on which breeding plans can be made.

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“…Jordan has only Awassi sheep of various ecotypes and strains that originated and were genetically developed for many past generations (Al-Atiyat et al, 2012). However, more information and details of Jordanian Awassi sheep breeds, including morphological, phenotypical and production characteristics and genetic diversity and structure, were reported.…”

Section: Introductionmentioning

confidence: 99%

Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

Migdadi

Haddad

Alomari

et al. 2021

IJAR

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Background: Jordanian Awassi sheep (Ovis aries) is the dominant fat tail sheep breed that appeals to customers because of its various production systems, including fiber, meat and milk. This report is the first whole ewe genome sequence (WGS) of O. aries submitted in the NCBI database from Jordan. Methods: 64 Paired-end sequencing libraries were constructed and subjected to Illumina Hiseq 2500 sequencing system. High-quality reads were aligned against the reference sheep genome and detecting comprehensive sources (SNPs, InDels, SV, CNVs) of genetic variations. We have deposited data sequences at the NCBI under SRA (sequence reads archives) under the accession numbers SRR11128863, PRJNA574879. Result: Genome resequencing of Jordanian Awassi ewe was carried out with approximately 93.88 Gb with a mapping rate and effective mapping depths were 99.28% and 36.32. Around 19 million SNPs, 3,6 million InDels, 35,180 Structure variation and 13,524 copy number variation among the Jordanian ewe genome were detected. This wide range of genetic variation provides a framework for further genetic studies that will help understand the molecular basis underlying phenotypic variation of economically important traits in sheep and improve intrinsic defects in domestic sheep breeds.

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Analysis of Genetic Variation of Fat Tailed-sheep in Southern Region of Jordan

Cited by 11 publications

References 21 publications

Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers

Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

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Analysis of Genetic Variation of Fat Tailed-sheep in Southern Region of Jordan

Cited by 11 publications

References 21 publications

Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers

​Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

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Product

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Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection