Analysis of histomorphometric and proteome dynamics inside the silk gland lumen of Bombyx mori revealed the dynamic change of silk protein during the molt stage

Guo, Kaiyu; Dong, Zhaoming; Zhang, Xiaolu; Chen, Yuqing; Li, Yang; Jiang, Wenchao; Qin, Lixia; Zhang, Yan; Guo, Zhouguanrui; Xia, Qingyou; Zhao, Ping

doi:10.1016/j.ijbiomac.2023.123926

International Journal of Biological Macromolecules

2023

DOI: 10.1016/j.ijbiomac.2023.123926

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Analysis of histomorphometric and proteome dynamics inside the silk gland lumen of Bombyx mori revealed the dynamic change of silk protein during the molt stage

Kaiyu Guo

Zhaoming Dong

Xiaolu Zhang

et al.

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Cited by 6 publications

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Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori

Hong,

Hu,

et al. 2024

Arch Insect Biochem Physiol

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Our previous research reported the influence of 50 μM selenium (Se) on the cytosolization (endocytosis) pathway, which in turn stimulates the growth and development of Bombyx mori. Lately, dynamin is recognized as one of the key proteins in endocytosis. To explore the underlying mechanisms of Se impact, the dynamin gene was knocked down by injecting siRNAs (Dynamin‐1, Dynamin‐2, and Dynamin‐3). This was followed by an analysis of the target gene and levels of silk protein genes, as well as growth and developmental indices, Se‐enrichment capacity, degree of oxidative damage, and antioxidant capacity of B. mori. Our findings showed a considerable decrease in the relative expression of the dynamin gene in all tissues 24 h after the interference and a dramatic decrease in the silkworm body after 48 h. RNAi dynamin gene decreased the silkworm body weight, cocoon shell weight, and the ratio of cocoon. In the meantime, malondialdehyde level increased and glutathione level and superoxide dismutase/catalase activities decreased. 50 μM Se markedly ameliorated these growth and physiological deficits as well as decreases in dynamin gene expression. On the other hand, there were no significant effects on fertility (including produced eggs and laid eggs) between the interference and Se treatments. Additionally, the Se content in the B. mori increased after the dynamin gene interference. The dynamin gene was highly expressed in the silk gland and declined significantly after interference. Among the three siRNAs (Dynamin‐1, Dynamin‐2, and Dynamin‐3), the dynamin‐2 displayed the highest interference effects to target gene expression. Our results demonstrated that 50 μM Se was effective to prevent any adverse effects caused by dynamin knockdown in silkworms. This provides practical implications for B. mori breeding industry.

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Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori

Hong,

Hu,

et al. 2024

Arch Insect Biochem Physiol

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CRISPR/Cas9-mediated gene editing of the let-7 seed sequence improves silk yield in the silkworm, Bombyx mori

Wang

Zhang

Guo

et al. 2023

International Journal of Biological Macromolecules

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A review on complete silk gene sequencing and de novo assembly of artificial silk

Lu,

Shi,

et al. 2024

International Journal of Biological Macromolecules

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Analysis of histomorphometric and proteome dynamics inside the silk gland lumen of Bombyx mori revealed the dynamic change of silk protein during the molt stage

Cited by 6 publications

References 66 publications

Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori

Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori

CRISPR/Cas9-mediated gene editing of the let-7 seed sequence improves silk yield in the silkworm, Bombyx mori

A review on complete silk gene sequencing and de novo assembly of artificial silk

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