Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements

Abstract

So far, Bacillus species bacteria are being used as bacteria concentrates, supplementing cleaning preparations in order to reduce odor and expel pathogenic bacteria. Here, we discuss the potential of Bacillus species as ‘natural’ probiotics and evaluate their microbiological characteristics. An industrial microbiological concentrate CS-4 of mixed Bacillus species cultures was tested, which may be a promising bacteria source for food probiotic preparation for supplementary diet. In this study, antagonistic activities and probiotic potential of Bacillus species, derived from an industrial microbiological concentrate, were demonstrated. The cell free supernatants (CFS) from Bacillus licheniformis mostly inhibited the growth of foodborne pathogenic bacteria, such as Escherichia coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, and Staphylococcus aureus KCCM 11335, while some of Bacillus strains showed synergistic effect with foodborne pathogenic bacteria. Moreover, Bacillus strains identified by the MALDI TOF-MS method were found sensitive to chloramphenicol, kanamycin, and rifampicin. B. licheniformis and B. cereus displayed the least sensitivity to the other tested antibiotics, such as ampicillin, ampicillin and sulfbactam, streptomycin, and oxacillin and bacitracin. Furthermore, some of the bacterial species detected extended their growth range from the mesophilic to moderately thermophilic range, up to 54 °C. Thus, their potential sensitivity to thermophilic TP-84 bacteriophage, infecting thermophilic Bacilli, was tested for the purpose of isolation a new bacterial host for engineered bionanoparticles construction. We reason that the natural environmental microflora of non-pathogenic Bacillus species, especially B. licheniformis, can become a present probiotic remedy for many contemporary issues related to gastrointestinal tract health, especially for individuals under metabolic strain or for the increasingly growing group of lactose-intolerant people.

Bacillus subtilis RBT-7/32 and Bacillus licheniformis RBT-11/17 as New Promising Strains for Use in Probiotic Feed Additives

“…New approaches developed in recent years for prevention and treatment of gastrointestinal diseases are based on the recovery of the natural microflora by probiotic preparations [40][41][42][43]. Bacillus bacteria represent promising strains for the development of probiotic additives; their probiotic activity is based on the synthesis of antimicrobial compounds, enhancement of a nonspecific and specific immunity, stimulation of the growth of the normal intestinal microflora, and production of digestive enzymes.…”

Probiotic-Based Sanitation in the Built Environment—An Alternative to Chemical Disinfectants

“…All published in situ evidence supports the claim that non-pathogenic Bacillus spp. are safe for humans in a variety of applications, including water treatment, agriculture, livestock, food preparation and gut homeostasis [88][89][90][91][92][93]. In vitro assessment of airborne Bacillus levels in indoor environments that were treated with Bacillus-based carpet cleaners estimated minimal risk associated with inhalation of Bacillus spores and cells [94].…”

Correction: Łubkowska et al. Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements. Microorganisms 2023, 11, 488

“…There was an error in the original publication [ 1 ]. There was a mistake in our analyzed sample sourse.…”