Analysis of lactase in lactose intolerance supplements

Abstract

Lactase is the enzyme responsible for the digestion of the disaccharide lactose, and deficiency in this enzyme causes the prevalent medical condition lactose intolerance. Management of lactose intolerance can be achieved through the administration of lactase supplements. Lactase is an appropriate platform for advanced enzymatic study because its medical application is a motivator for student learning. The following is an upper‐level biochemistry laboratory sequence that integrates student inquiry and exposure to advanced laboratory techniques. Students investigate three different lactase supplements through experimentation that includes the Bradford assay, SDS‐PAGE, continuous and discontinuous kinetic assays, and zymography. Upon completion of this project, students compile their results and conclusions in a scientifically formatted paper comparing supplement protein content and activity. This safe and inexpensive laboratory project enriches student understanding of key biochemical concepts while mirroring work performed in a realistic research setting. © 2018 International Union of Biochemistry and Molecular Biology, 46(6):652–662, 2018.

Investigating thermal stability based on the structural changes of lactase enzyme by several orthogonal methods

“…Despite the pharmaceutical and commercial relevance of the lactase containing products [ 19 ], our knowledge is limited about the relationship between their structure and activity. In the present study we will use a combination of analytical tools in order to discover structural changes caused by stress conditions, which influence enzyme activity and function.…”

Optimization of Extraction Process of Polysaccharides MAP-2 from Opuntia Milpa Alta by Response Surface Methodology and Evaluation of Its Potential as α-Glucosidase Inhibitor

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Exploring Biochemical Reactions of Proteins, Carbohydrates, and Lipids through a Milk-Based Demonstration and an Inquiry-Based Worksheet: A COVID-19 Laboratory Experience

“…Some tasks give students opportunities to explore real-world applications of chemistry in fields ranging from healthcare to engineering. ,− Other teaching methods highlight the interplay between the disciplines. − Strategies such as these promote curiosity and immersion into the subject matter, allowing students to practice genuine scientific investigation through engaging in activities where outcomes are unknown. − Educators and researchers hold that this approach greatly enhances the pedagogical value of laboratory exercises − because (1) it allows for greater discovery beyond that attributed to the “cookbook experience” of traditional laboratories and (2) it facilitates greater collaboration between students and interaction between students and their instructors, thus enhancing the students’ postsecondary experience . These pedagogies have many well-documented successes: learning activities involving inquiry, guided or open, and real-world applications produce greater effectiveness in learning. ,,,− It should not be of any surprise, then, that many postsecondary institutions have already adopted an inquiry-based approach in face-to-face learning.…”