Analysis of lipid biomarkers in rocks of Archean crystalline basement

Shekhovtsova, Nina V.; Осипов, Г. А.; Верховцева, Н. В.; Pevzner, L. A.

doi:10.1117/12.486691

Cited by 7 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The taxonomic structure of microbial communities was determined by gas chromatography-mass spectrometry (GC-MS) of lipids. The analysis was carried out on the GC-MS system HP-5973 Agilent Technologies (USA) using the technique of multi-ion mass fragmentography of specific ions of mass spectra of genera- and species-specific higher fatty acids (FA) and cell wall aldehydes [50],[51].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Microbial activity in Martian analog soils after ionizing radiation: implications for the preservation of subsurface life on Mars

et al. 2018

View full text Add to dashboard Cite

At present, the surface of Mars is affected by a set of factors that can prevent the survival of Earth-like life. However, the modern concept of the evolution of the planet assumes the existence more favorable for life climate in the past. If in the past on Mars had formed a biosphere, similar to the one that originated in the early Earth, it is supposed that it is preserved till now in anabiotic state in the bowels of the planet, like microbial communities inhabiting the ancient permafrost of Arctic and Antarctic. In the conditions of modern Martian regolith, this relic life seems to be deprived of the possibility of damage reparation (or these processes occur on a geological time scale), and ionizing radiation should be considered the main factor inhibiting such anabiotic life. In the present study, we studied soil samples, selected in two different extreme habitats of the Earth: ancient permafrost from the Dry Valleys of Antarctica and Xerosol soil from the mountain desert in Morocco, gamma-irradiated with 40 kGy dose at low pressure (1 Torr) and low temperature (−50 °C). Microbial communities inhabiting these samples showed in situ high resistance to the applied effects, retained high number of viable cells, metabolic activity, and high biodiversity. Based on the results, it is assumed that the putative biosphere could be preserved in the dormant state for at least 500 thousand years and 8 million years in the surface layer of Mars regolith and at 5 m depth, respectively, at the current level of ionizing radiation intensity.

show abstract

Section: Methodsmentioning

confidence: 99%

“…To search for minor biogenic chemical components, mass-fragmentography method was used [50]. The program of multi-ion detection with accumulation of signals of 85 specific ions of fatty acids, aldehydes and sterols—marker substances of microorganisms was used.…”

Section: Methodsmentioning

confidence: 99%

Microbial activity in Martian analog soils after ionizing radiation: implications for the preservation of subsurface life on Mars

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The taxonomic structure of microbial communities in situ was studied by gas chromatography-mass spectrometry (GC-MS) of lipids for the Xerosol samples due to its more pronounced reaction according to the results of culturing, EFM, FISH and MST compared to the permafrost samples. The analysis was carried out on the GC-MS system HP-5973 Agilent Technologies (USA) using the technique of multi-ion mass fragmentography of specific ions of mass spectra of genera- and species-specific higher fatty acids and cell wall aldehydes (Turova and Osipov, 1996; Shekhovtsova et al ., 2003). Detailed description of sample preparation and analysis are presented in Cheptsov et al .…”

Section: Methodsmentioning

confidence: 99%

Survival and growth of soil microbial communities under influence of sodium perchlorates

Cheptsov

Belov

Soloveva

et al. 2020

International Journal of Astrobiology

View full text Add to dashboard Cite

Previously conducted space missions revealed the presence of perchlorates, which are known to have a high oxidizing potential in Martian regolith, at the level of 0.5%. Due to hygroscopic properties and crystallization features of perchlorate-containing solutions, assumptions leading to the possibility of the existence of liquid water in the form of brines, which can contribute to the vital activity of microorganisms, have been made. At the same time, high concentrations of perchlorates can inhibit the growth of microorganisms and cause their death. Previously performed studies have discovered the presence of highly diverse microbial communities in terrestrial perchlorate-containing soils and have also demonstrated the stability and activity of some prokaryotes cultured on highly concentrated perchlorates media (over 10%). Nevertheless, the limits of microbial tolerance to perchlorates and whether microbial communities are able to withstand the effects of high concentrations of perchlorates remain uncertain. The aim of this research was to study the reaction of microbial communities of hot-arid and cryo-arid soils and sedimentary rocks to the adding of a highly concentrated solution of sodium perchlorate (5%) in situ. An increase in the total number of prokaryotes, the number of metabolically active Bacteria and Archaea, and the variety of the consumed substrates were revealed. It was observed that in samples incubated with sodium perchlorate, a high taxonomic diversity of the microbial community is preserved at a level comparable to control sample. The study shows that the presence of high concentrations of sodium perchlorate (5%) in the soil does not lead to the death or significant inhibition of microbial communities.

show abstract

“…The methodology of a molecular method of a gas chromatography-mass spectrometry (GC-МS) was in detail presented by Refs. [13,14].…”

Section: Methodsmentioning

confidence: 99%

Mobilization of Phosphorus, Potassium and Silicon in the Greenhouse Ground at Application of Bacterial Preparations

Kiryushin¹,

Пашкевич²,

Neymatov³

et al. 2011

JAST-A

View full text Add to dashboard Cite

The investigation of P, K and Si mobilization dynamics in greenhouse ground with the application of selective microorganisms and diatomite was the purpose of this work. The substrate for laboratory research was ground (peat and perlite mixture-PP), diatomite (D) and bacterial preparations, which were introduced into the ground. Four bacteria isolated of environment places: from soils-Bacillus macerans and Bacillus circulans and from surface of sand-Bacillus circulans 2 and Corynebacterium sp. have been applied as a basis of bacterial preparations. Experiment included 10 variants: two grounds (PP and PPD) and 8 variants with treating ground of each of four bacterial preparations. The results showed that the content of water-soluble elements forms in a ground with microorganisms after 56 days has increased: P and K-in 10 times, Si-in 3 times. It proved efficiency of bacterial cultures in the mobilization of these elements. The most active microorganisms were those that have been isolated from surface of sand (Corynebacterium sp. and Bacillus circulans 2). The mixture of ground and diatomite showed the best efficiency. The best ground microbes' community was investigated using chemo diagnostic method gas chromatography-mass spectrometry. It has been shown that the content of Acetobacterium sp. (the culture capable to formation of acetic acid in an anaerobic metabolism), had increased to the end of composting period in 10 times and the number of Bacillus sp.-as minimum in 100 times.

show abstract

Analysis of lipid biomarkers in rocks of Archean crystalline basement

Cited by 7 publications

References 0 publications

Microbial activity in Martian analog soils after ionizing radiation: implications for the preservation of subsurface life on Mars

Microbial activity in Martian analog soils after ionizing radiation: implications for the preservation of subsurface life on Mars

Survival and growth of soil microbial communities under influence of sodium perchlorates

Mobilization of Phosphorus, Potassium and Silicon in the Greenhouse Ground at Application of Bacterial Preparations

Contact Info

Product

Resources

About