Analysis of Lysate Platelet-Rich Fibrin Effects on Human Dental Pulp Stem Cell Differentiation Through Dentin Sialophosphoprotein Expression

Abstract

Objective: In vitro, the culture media in which human dental pulp stem cells (hDPSCs) are grown are supplemented with specific growth factors thatinduce cell cycle entry and differentiation. Lysate platelet-rich fibrin (L-PRF) is a unique and stable growth factor supplement produced from plateletslysed by freezing-thawing. In this study, we aimed to analyze the potential effects of L-PRF on hDPSC differentiation.Methods: We divided hDPSCs isolated from human third molars at the second passage into five culture media groups treated with 1%, 5%, 10%,and 25% L-PRF or 10% fetal bovine serum (control). After 7 days, we evaluated hDPSC differentiation using an enzyme-linked immunosorbent assayspecific for dentin sialophosphoprotein and Alizarin-Red staining.Results: None of our analyses revealed any significant differences between the L-PRF- and control-treated cells.Conclusion: L-PRF could potentially induce the differentiation of hDPSCs in vitro.

Changes in Migratory Speed Rate of Human Dental Pulp Stromal Cells Cultured in Advanced Platelet-Rich Fibrin

“…23 The result of this study was in conjunction with the results of Saeed et al and Swastiningtyas et al, which showed a greater effect on low proliferation of L-PRF. 22,30,31 Saeed et al studied the efficacy of hPRF exudate (compression without freeze and thaw methods) and showed that 10% of hPRF is the optimum concentration for hDP-SCs' proliferation, but did not show the efficacy for migration of hDP-SCs.22. 22 The study by Utami Dewi et al showed that various concentrations of L-PRF have different effects on migration rate of DPSCs.…”