Analysis of metabolites in young and mature Docynia delavayi (Franch.) Schneid leaves using UPLC-ESI-MS/MS

Abstract

Docynia delavayi (Franch.) Schneid is a plant used both as food and traditional folk medicine. The leaves of D. delavayi are rich in polyphenols, plants with phenolic content are known to be extremely beneficial in terms of human nutrition. In the present study, we used metabolome technology (UPLC-ESI-MS/MS) to examine the young and mature D. delavayi leaves on metabolites changes, which were then analyzed and compared. As a result, 477 metabolites (including 111 flavonoids, 47 others (consisted of nine vitamin, 18 saccharides and alcohols, and 20 unassigned metabolites), 71 phenolic acids, 52 amino acids and derivatives, 18 alkaloids, 61 lipids, 24 terpenoids, 33 nucleotides and derivatives, 18 lignans and coumarins, 12 tannins, 30 organic acids) were identified, of which 281 differentially accumulated metabolites, including 146 up-regulated metabolites and 135 down-regulated metabolites. The result of clustering and PCA analyses showed that young and mature leaves were separated, which indicated that there was a great difference in metabolites between young and mature leaves. Meanwhile, we also found that both young and mature leaves displayed unique metabolites with important biological functions. KEGG enrichment analysis showed that 90 of the differential metabolites were mainly concentrated in 68 KEGG pathways. The result will greatly complement the existing knowledge on the D. delavayi leaves for lays a foundation for subsequent development and utilization.

Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves

“…Various findings have shown that the composition of secondary metabolites differs in young and mature leaves [ 52 , 54 , 55 , 77 , 86 ]. Recent studies have found that Gingko biloba L. [ 52 ] and sugarcane [ 55 ] contain higher secondary metabolites in older leaves than in immature leaves.…”

Metabolomics study of flavonoids in Coreopsis tinctoria of different origins by UPLC–MS/MS

“…YT, TX vs. SY and YT vs. SY comparisons suggested that the metabolite profile of TX was notably distinguishable from those of the other two varieties. It is strongly believed that flavonoids in plant tissues can improve the stress resistance of plants and protect them from abiotic stresses, such as UV-B radiation and drought ( Harborne & Williams, 2000 ; Xia et al, 2022 ). A previous study reported that flavonoids may function as antioxidants in response to excessive light exposure ( Tattini et al, 2004 ).…”

Metabonomics Analysis of Stem Extracts from Dalbergia sissoo

“…Multivariate statistical analysis was performed using ropls (Version1.6.2, , accessed on 8 March 2021) R package from Bioconductor on the Majorbio Cloud Platform ( , accessed on 8 March 2021). A PCA using an unsupervised method was applied to obtain and visualize an overview of the metabolic data, general clustering, trends, or outliers [ 54 ]. All metabolite variables were scaled to unit-variances prior to conducting the PCA.…”