Analysis of Methanolic Fruit Extract of <i>Citrus aurantifolia</i> Using Gas Chromatography-Mass Spectrum and FT-IR Techniques and Evaluation of Its Anti-bacterial Activity

Hameed, Rafid Hadi; Shareefi, Ekhlas Al; Hameed, Imad Hadi

doi:10.5958/0976-5506.2018.00490.4

Cited by 12 publications

(9 citation statements)

References 29 publications

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“…Major compounds in methanol and hexane extracts like presilphiperfolan-1-ol [ 32 ], nalidixic acid [ 33 ], phytol [ 34 ], gamma-sitosterol [ 35 ], α-amyrin [ 36 ] are reported to be antibacterial/anti-fungal along with other properties. It has been reported that heptatriacontanol gives the antimicrobial activity to methanol extract of Trilepisium madagascariense [ 37 ] and cholestan-3-ol, 2-methylene-, is the compound present in the antibacterial fraction of Z.officianale [ 38 ] and Citrus aurantifolia1 [ 39 ] These compounds were identified from the hexane extract of O. thymiflorus . The antibacterial, anti fungal activity of the compounds detected from the extract justify the use of the plant in traditional medicine against skin diseases.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of genoprotection against malathion induced toxicity by Orthosiphon thymiflorus Sleesen

R¹,

Srinivas

Thoppil

2021

Journal of Ayurveda and Integrative Medicine

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Background Pesticide toxicity is considered to be one of the significant reason for increased incidence of cancer. Plants are treasure troves of active phytochemical compounds which are used as herbal medicine as well as nutraceuticals. Objective To evaluate the genoprotective potential of Orthosiphon thymiflorus (Roth) Sleesen, (Lamiaceae) against the toxicity induced by malathion by a battery of four in vivo assays in Swiss albino mice. Materials and methods Micronucleus assay was performed for analyzing the micronuclei induction and ratio of polychromatic and normochromatic erythrocytes (PCE/NCE). Anticlastogenic and mito depressive effect of the methanol and hexane extracts of O.thymiflorus were evaluated by chromosome aberration assay. Alkali comet assay was performed to assess double strand DNA repair. DNA damage sensing ability of the bone marrow cells were assessed by γ-H2AX foci formation. Phytochemical screening of hexane and methanol extract was done by GC–MS analysis. Result O. thymiflorus extracts showed a dose dependant protective effect in all assays. It significantly decreased the frequency of micronuclei and improved PCE/NCE value in post treated groups of animals. Malathion induced clastogenic aberrations were effectively attenuated by methanol and hexane extracts. DNA comet assay showed that malathion induced damage can be protected by O. thymiflorus extracts. Multiple foci formation in γ-H2AX assay confirmed the activation of DNA repair proteins in post treated animals. Conclusion Genoprotective effect of O. thymiflorus against malathion induced toxicity was confirmed. This study would be helpful to initiate more research including clinical using O. thymiflorus extract against pesticide induced toxicity.

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Section: Discussionmentioning

confidence: 99%

Evaluation of genoprotection against malathion induced toxicity by Orthosiphon thymiflorus Sleesen

R¹,

Srinivas

Thoppil

2021

Journal of Ayurveda and Integrative Medicine

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“…Very interestingly, 2-vinyl-9-[beta-D-ribofuranosyl] hypoxanthine was detected in ethyl acetate extract of Rhizopus oryzae. It has previously been reported from the methanolic fruit extract of Citrus aurantifolia [39]. Production of bioactive compounds in endophytic organisms can be linked with the medicinal properties of plants, and plants may have a role in initiating production of these metabolites due to specific environment offered to fungal strains [64,65].…”

Section: Discussionmentioning

confidence: 99%

Identification and Bioactivities of Two Endophytic Fungi Fusarium fujikuroi and Aspergillus tubingensis from Foliar Parts of Debregeasia salicifolia

et al. 2020

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Endophytic fungi isolated from medicinal plants are important for production of antibiotics. They can produce secondary metabolites with diverse structures and activities. Debregeasia salicifolia is a plant of medicinal importance, and no report exists regarding isolation of endophytic fungi from it. This study was focused to isolate and identify culturable endophytic fungi from foliar parts of D. salicifolia and to determine their bioactivities. Molecular analysis resulted in identification of Fusarium fujikuroi, Aspergillus tubingensis and Rhizopus oryzae based on specific internal transcribed spacer primer (ITS1/ ITS4). Our analysis revealed that all fungal endophytes possess antibacterial activity against Gram-negative and Grampositive bacteria. Remarkably, Rhizopus oryzae at a concentration of 5 mg/mL efficiently restricted the growth of ATCC strain of E. coli in comparison with positive control ciprofloxacin. Rhizopus oryzae and F. fujikuroi at a concentration of 1000 µg/ml exhibited maximum antioxidant activity of 45% and 44%, respectively. They also showed antifungal activity ranging from 60 to 75% against Aspergillus flavus and Aspergillus niger. Our analysis of the fungal extracts through GC-MS indicated the presence of 21 compounds of diverse nature and structure. In conclusion, our study highlighted the potential of D. salicifolia to host a plethora of fungal endophytes that secrete potentially therapeutic bioactive metabolites

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“…Studies on EPEC revealed induced rearrangements of the cytoskeleton, which have begun to provide various conclusions. The mechanisms involved in the appearance of such a phenomenon used by this pathogen were to undermine the host cell cytoskeleton and signaling pathways [9,24] .…”

Section: Discussionmentioning

confidence: 99%

Binding of enteropathogenic Escherichia coli (EPEC) strains to glycolipid receptors isolated from human tissues

Milh¹,

Musa²,

Alawzy³

2020

ATMPH

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A number of Escherichia coli strains isolated from patients suffering from diarrhea were characterized and isolated. They were classified according to their activity of recognition of specific glycosphingolipid-receptors separated on thin-layer chromatogram (TLC) and those incorporated into liposomes. Our finding revealed that 20% of tested strains (group 1) bound Galα1-4Gal-containing glycosphingolipids found in total natural fractions of human meconium and human stomach as well as reference glycosphingolipids. The majority of EPEC strains (group 2) recognized clearly GalNAcβ4Gal-containing receptors within these fractions. Using lipid extracts from total natural fractions of the stomach and meconium, group 1 bound an actively large number of glycosphingolipid receptors demonstrated by intensified black bands, whereas two positive bindings in the 2-and 4-sugar-regions were only detected when EPEC group 2 was used. Application of liposomeaggregation binding assay, the two selected strains EPEC 37 and EPEC 57, representing group 1 and group 2, respectively, recognized well Galα1-4Gal and GalNAcβ4Gal-containing glycosphingolipids incorporated into liposomes receptors. In the present study, lactosylceramideglycosphingolipid (Galβ4Glcβ1Cer) with nonhydroxylated ceramide showed no binding activity in both assay-systems, whereas the same receptorcontaining hydroxylated fatty acid of ceramide was bound by both strain groups.

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Analysis of Methanolic Fruit Extract of Citrus aurantifolia Using Gas Chromatography-Mass Spectrum and FT-IR Techniques and Evaluation of Its Anti-bacterial Activity

Cited by 12 publications

References 29 publications

Evaluation of genoprotection against malathion induced toxicity by Orthosiphon thymiflorus Sleesen

Evaluation of genoprotection against malathion induced toxicity by Orthosiphon thymiflorus Sleesen

Identification and Bioactivities of Two Endophytic Fungi Fusarium fujikuroi and Aspergillus tubingensis from Foliar Parts of Debregeasia salicifolia

Binding of enteropathogenic Escherichia coli (EPEC) strains to glycolipid receptors isolated from human tissues

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