Analysis Of MYO1H Single Nucleotide Polymorphism In Class III Malocclusion With Mandibular Prognathism: A Preliminary Study

Abstract

Introduction: Evidence suggests that several genes; including MYO1H, play an important role in the etiology of Class III malocclusion. Single nucleotide polymorphism (SNP) in marker rs10850110 (locus 12q24.11) within MYO1H gene has been associated with the incidence of mandibular prognathism (MP). MYO is a class 1 myosin that is responsible for the synthesis of Matrilin-1; an important protein involved in the formation of cartilage's extracellular matrix, hence is implicated in the formation of mandibular condyle cartilage. This study aimed to detect the presence of MYO1H (rs10850110) SNP and to determine its genotype and allele distribution in MP patient in the local population. Materials and Methods: The sample comprises of 31 patients; 14 patients from class I malocclusion (control samples) and 17 patients from class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs10850110 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p<0.05). Results: Presence of MYO1H SNP (rs10850110) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within control (Class I) and Class III malocclusion. Conclusion(s): Our findings are in agreement with previous studies suggesting positive influence of MYO1H (rs10850110) SNP in the incidence of MP. Further studies should be developed in order to understand the exact role and mechanism of MYO1H in different classes of malocclusions.

Analysis of MYO1H Gene Polymorphism in Skeletal Class-III Malocclusion Due to Mandibular Prognathism

“…Cruz et al 31 used the TaqMan method of real-time PCR to amplify the genome and demonstrated that the polymorphism of rs10850110 in MYO1H, GHR, and FGF genes plays a role in mandibular prognathism and maxillomandibular discrepancies. Yahya et al 32 on the Malay population also showed the role of SNP (rs10850110) MYO1H in class-III mandibular prognathism patients as a risk factor. Da Fontoura et al on Romanian and American populations showed that the G allele of rs10850110 polymorphism of the MYO1H gene being overrepresented in skeletal class-III patients.…”

The association of polymorphisms in BMP2/MYO1H and skeletal Class II div.1 maxillary and mandibular dimensions. A preliminary ‘report

“…MYO1H was previously identified to be associated with mandibular prognathism or mandibular retrognathism ( Yahya et al, 2007 , Arun et al, 2016 ). Previous researches mainly focused on Class III malocclusion.…”

“…Previous researches mainly focused on Class III malocclusion. Yahya et al , reported the association of MYO1H rs10850110 and mandibular prognathism in the Malaysian population ( Yahya et al, 2007 ). Conversely, Dalaie et al, reported in the Iranian population a non-significant correlation between MYO1H rs10850110 and skeletal class III due to mandibular prognathism ( Dalaie et al, 2020 ) and the same finding was reported in the Brazilian population by Cunha ( Cunha et al, 2019 ).…”

“…In additin, SNPs in certain genes have been associated with various types of malocclusions. Previous studies focused mainly on genetic polymorphisms and class III malocclusion with limited knowledge on SNPs and class II division 1 maloclclusion ( Yahya et al, 2007 ). As the aforementioned genes have been reported to be associated with craniofacial bone and cartilage formation; thus we hypothesize that they could offer a genetic contribution with Class II division 1 malocclusion.…”