Analysis of periodontitis-associated miRNAs in gingival tissue, gingival crevicular fluid, saliva and blood plasma

Rovas, Adomas; Pūrienė, Alina; Snipaitiene, Kristina; Punceviciene, Egle; Buragaite-Staponkiene, Benita; Matuleviciute, Ruta; Butrimienė, İrena; Jarmalaitė, Sonata

doi:10.1016/j.archoralbio.2021.105125

Cited by 13 publications

(13 citation statements)

References 25 publications

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“…In agreement, a pilot study by Saito et al, 17 in which both periodontitis and healthy subjects were similarly matched for age and sex, found significant unbalanced expression of several GCF miRNAs profiling. 17 Regarding smoking, different from the present study results, some reports 17,18 showed that smoking habits might influence the expression of GCF miRNAs. 28 This may be because long-term smoking habits could negatively impact wound healing, and extracellular matrix protein homeostasis and accelerate cellular senescence of gingival fibroblasts through specific mechanisms involving some miRNAs, which code the type I and III collagen, interleukins and p53.…”

Section: Discussioncontrasting

confidence: 99%

“…The multivariate linear regression analysis aimed at identifying possible predictors of GCF miRNAs expression highlighted that, among the analyzed confounders, the extent of periodontitis (PISA) represented one of the main significant predictors of several GCF miRNAs expression. In agreement with the present results, it has been previously shown that the local miRNAs upregulation may be directly correlated to the extent of periodontitis 36 and also that the severity of periodontitis could in turn influence the serum (miRNA‐199a‐5p) and local levels of several miRNAs in both gingival tissues (miRNA 199a‐5p, ‐483‐5p, ‐3198, ‐4299), GCF (miRNA‐140‐3p, ‐145‐5p, 146a‐5p, ‐3198, ‐4299) and saliva (miRNA‐381‐3p) 18,37 …”

Section: Discussionsupporting

confidence: 92%

“…11 More specifically, miRNA-7, 15 miRNA-100, and miRNA-125 16 have been related to the release of inflammatory cytokines and metalloproteases in gingival fibroblasts during the early stages of periodontitis, suggesting the critical modulatory role that miRNAs could play during the early stages and subsequent progression of the disease. 17 Moreover, a preliminary study by Rovas et al, 18 who analyzed miRNA profiles in subjects affected by periodontitis and CVD, found higher miRNA-3198 and miRNA-4299 levels compared with healthy controls. In agreement, Bagavad Gita et al 19 evidenced upregulated miRNA-146a concentrations in subgingival plaque of subjects with periodontitis, suggesting a possible role of miRNAs in the early immune response seen during periodontitis and CVD.…”

Section: Introductionmentioning

confidence: 99%

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Impact of periodontitis on gingival crevicular fluid miRNAs profiles associated with cardiovascular disease risk

Isola

Santonocito

Distefano

et al. 2022

J of Periodontal Research

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Background and Objective Recent emerging evidence has shown that microRNA (miRNAs) is involved in several epigenetic processes linked with periodontitis, increased oxidative stress and cardiovascular disease (CVD). The present study aimed to assess the impact of periodontitis on gingival crevicular fluid (GCF) miRNAs expression associated with CVD risk and to evaluate possible confounders that influenced this association. Materials and Methods For the present study, healthy controls (n = 28) and subjects with CVD (n = 28), periodontitis (n = 30) and periodontitis + CVD (n = 29) were enrolled. All subjects underwent regular periodontal examinations and blood sampling. In addition, GCF sampling was performed, and miRNAs 7a‐5p, 21‐3p, 21‐5p, 100‐5p, 125‐5p, 200b‐3p, and 200b‐5p expression was analyzed using a real‐time quantitative polymerase chain reaction (RT‐PCR). Results The results showed that periodontitis and periodontitis + CVD subjects presented significantly different GCF miRNAs expression compared to healthy controls and CVD subjects. More specifically, compared to healthy controls and CVD, subjects with periodontitis and periodontitis + CVD showed higher GCF miRNA 7a‐5p, miRNA 21‐3p, miRNA 21‐5p, miRNA 200b‐3p, and miRNA 200b‐5p (p < .05) and lower miRNA 100‐5p, miRNA 125‐5p levels (p < .05). Furthermore, the multivariate regression analysis evidenced that periodontitis (miRNA 21‐3p, 100‐5p) and periodontal inflamed surface area (PISA) (miRNA 7a‐5p, 21‐3p, 21‐5p, 100‐5p, 125‐5p, 200b‐3p) were significant predictors of GCF miRNAs concentration (p < .05). Conclusion The results of the study highlighted that the periodontitis and periodontitis + CVD group showed higher GCF miRNAs expression than healthy controls and CVD subjects. Furthermore, periodontitis and its extent (PISA) were revealed as significant predictors of GCF miRNAs associated with CVD risk.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of periodontitis on gingival crevicular fluid miRNAs profiles associated with cardiovascular disease risk

Isola

Santonocito

Distefano

et al. 2022

J of Periodontal Research

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“…In recent years, miRNAs have been implied to be biomarkers of certain diseases, as they are altered in several pathological events, and can be released by apoptotic and necrotic cells [ 36 , 37 ]. Although miRNAs have an intracellular function, they have already been identified in several biological fluids, such as cerebrospinal fluid [ 38 ], urine [ 39 ], plasma [ 40 ] and serum [ 41 ], gingival fluid, and saliva [ 42 ]. Under these conditions, circulating miRNAs may be associated with proteins [ 43 ], microvesicles, or lipoprotein complexes [ 44 ].…”

Section: Mirna As Circulating Biomarkers Of Diseasementioning

confidence: 99%

MicroRNA in Acromegaly: Involvement in the Pathogenesis and in the Response to First-Generation Somatostatin Receptor Ligands

Henriques

Lamback

Dezonne

et al. 2022

IJMS

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Acromegaly is a chronic and systemic disease due to excessive growth hormone and insulin-like growth factor type I caused, in the vast majority of cases, by a GH-secreting pituitary adenoma. About 40% of these tumors have somatic mutations in the stimulatory G protein alpha-subunit 1 gene. The pathogenesis of the remaining tumors, however, is still not fully comprehended. Surgery is the first-line therapy for these tumors, and first-generation somatostatin receptor ligands (fg-SRL) are the most prescribed medications in patients who are not cured by surgery. MicroRNAs are small, non-coding RNAs that control the translation of many mRNAs, and are involved in the post-transcriptional regulation of gene expression. Differentially expressed miRNAs can explain differences in the pathogenesis of acromegaly and tumor resistance. In this review, we focus on the most validated miRNAs, which are mainly involved in acromegaly’s tumorigenesis and fg-SRL resistance, as well as in circulating miRNAs in acromegaly.

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“…However, neither the mimic nor the miR-3198 inhibitor had an impact on RANKL expression [75]. According to an investigation of gingival crevicular fluid, miR-3198, particularly when combined with miR-4299, exhibited a notable sensitivity and specificity for evaluating periodontitis [98].…”

Section: Mir-3198mentioning

confidence: 99%

Role of mechano-sensitive non-coding RNAs in bone remodeling of orthodontic tooth movement: recent advances

Yan

Liao

2022

Prog Orthod.

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Orthodontic tooth movement relies on bone remodeling and periodontal tissue regeneration in response to the complicated mechanical cues on the compressive and tensive side. In general, mechanical stimulus regulates the expression of mechano-sensitive coding and non-coding genes, which in turn affects how cells are involved in bone remodeling. Growing numbers of non-coding RNAs, particularly mechano-sensitive non-coding RNA, have been verified to be essential for the regulation of osteogenesis and osteoclastogenesis and have revealed how they interact with signaling molecules to do so. This review summarizes recent findings of non-coding RNAs, including microRNAs and long non-coding RNAs, as crucial regulators of gene expression responding to mechanical stimulation, and outlines their roles in bone deposition and resorption. We focused on multiple mechano-sensitive miRNAs such as miR-21, - 29, -34, -103, -494-3p, -1246, -138-5p, -503-5p, and -3198 that play a critical role in osteogenesis function and bone resorption. The emerging roles of force-dependent regulation of lncRNAs in bone remodeling are also discussed extensively. We summarized mechano-sensitive lncRNA XIST, H19, and MALAT1 along with other lncRNAs involved in osteogenesis and osteoclastogenesis. Ultimately, we look forward to the prospects of the novel application of non-coding RNAs as potential therapeutics for tooth movement and periodontal tissue regeneration.

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Analysis of periodontitis-associated miRNAs in gingival tissue, gingival crevicular fluid, saliva and blood plasma

Cited by 13 publications

References 25 publications

Impact of periodontitis on gingival crevicular fluid miRNAs profiles associated with cardiovascular disease risk

Impact of periodontitis on gingival crevicular fluid miRNAs profiles associated with cardiovascular disease risk

MicroRNA in Acromegaly: Involvement in the Pathogenesis and in the Response to First-Generation Somatostatin Receptor Ligands

Role of mechano-sensitive non-coding RNAs in bone remodeling of orthodontic tooth movement: recent advances

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