Analysis of phytosterol by MALDI-TOF mass spectrometry

Li, Yueyan; Liang, Miao; Shu, Jinian; Zhang, Peng; Sun, Wanqi

doi:10.1039/c4ay00383g

Cited by 3 publications

(4 citation statements)

References 54 publications

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“…The choice of matrix and the laser energy employed during MALDI plays a key role for the overall ionization efficiency. Laser energy (4–42 mJ cm –2 ) and choice of matrix [2,4-Dihydroxybenzoic acid (2,4-DHB), 2,5-dihydroxybenzoic acid (2,5-DHB), a-cyano-4-hydroxycinnamic acid (CHCA), dithranol] were evaluated for the analysis of β-sitosterol . The predominant ion observed was [M – H 2 O] + at m / z 396.6 although other ions such as [M + H – H 2 O] + , [M + Na] + , and [M + K] + were also observed, albeit in low intensity.…”

Section: Liquid Chromatography-mass Spectrometry (Lc-ms)mentioning

confidence: 99%

“…Laser energy (4−42 mJ cm −2 ) and choice of matrix [2,4-Dihydroxybenzoic acid (2,4-DHB), 2,5dihydroxybenzoic acid (2,5-DHB), a-cyano-4-hydroxycinnamic acid (CHCA), dithranol] were evaluated for the analysis of βsitosterol. 136 The predominant ion observed was [M − H 2 O] + at m/z 396.6 although other ions such as [M + H − H 2 O] + , [M + Na] + , and [M + K] + were also observed, albeit in low intensity. The four matrixes produced similar detectable peaks with 2,4-DHB giving the highest intensity (five times that of CHCA), dithranol had the lowest peak intensity and high chemical noise while a high laser intensity was found to induce oxidation.…”

Section: ■ Introductionmentioning

confidence: 96%

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Mass Spectrometric Approaches for the Analysis of Phytosterols in Biological Samples

Gachumi

El‐Aneed

2017

J. Agric. Food Chem.

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Plant sterols (phytosterols) are important structural components of plant cellular membranes, and they play a major role during development and metabolism. They have health-associated benefits, especially in lowering blood cholesterol levels. Because of their many health claims, there is a growing interest in their analysis. Although various analytical strategies have been employed in analyzing phytosterols, chromatography linked to mass spectrometry (MS) is superior due to its sensitivity. Furthermore, specificity and selectivity are enhanced by utilizing tandem mass spectrometry (MS/MS). This article reviews the various mass spectrometric strategies used for the analysis of phytosterols. It highlights the applications and limitations associated with each MS strategy in various sample matrixes such as plant, human, animal, food, and dietary supplements. GC-MS was historically the method of choice for analysis; however, the derivatization step rendered it tedious and time-consuming. On the other hand, liquid chromatography coupled to MS (LC-MS) simplifies the analysis. Many ionization techniques have been used, namely, electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI). APCI showed superiority in terms of ion intensity and consistency in ion formation, primarily forming [M + H - HO] ions rather than [M + H]. In addition, matrix assisted laser desorption ionization (MALDI) as well as ambient mass spectrometry such as direct analysis in real time (DART) have also been evaluated.

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Section: Liquid Chromatography-mass Spectrometry (Lc-ms)mentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 96%

Mass Spectrometric Approaches for the Analysis of Phytosterols in Biological Samples

Gachumi

El‐Aneed

2017

J. Agric. Food Chem.

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“…Mass spectrometry is arguably the most ubiquitously used technique in the analysis of sterols [12]. While historically GC-MS technique in combination with suitable derivatisation has been the analytical MS technique of choice [13][14][15], today electrospray ionization (ESI) MS [16] and especially matrixassisted laser desorption/ionization (MALDI) [17][18][19] are increasingly used to analyze sterols [12]. However, because of poor ion yields, the analysis of neutral sterols like cholesterol or phytosterols is challenging with these techniques.…”

Section: Introductionmentioning

confidence: 99%

“…Detecting the same water-loss ion species as SIMS, MALDI-MS, and MSI of underivatized neutral sterols like cholesterol in positive ion mode has been reported with relatively poor sensitivity using different matrices [17][18][19]33]. To improve the ion yields for sterols in a MALDI-MS(I) analysis, MALDI-2, a recently introduced laser post-ionization technique, can be employed.…”

Section: Introductionmentioning

confidence: 99%

Molecular insights into symbiosis—mapping sterols in a marine flatworm-algae-system using high spatial resolution MALDI-2-MS imaging with ion mobility separation

et al. 2020

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Waminoa sp. acoel flatworms hosting Symbiodiniaceae and the related Amphidinium dinoflagellate algae are an interesting model system for symbiosis in marine environments. While the host provides a microhabitat and safety, the algae power the system by photosynthesis and supply the worm with nutrients. Among these nutrients are sterols, including cholesterol and numerous phytosterols. While it is widely accepted that these compounds are produced by the symbiotic dinoflagellates, their transfer to and fate within the sterol-auxotrophic Waminoa worm host as well as their role in its metabolism are unknown. Here we used matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging combined with laser-induced post-ionization and trapped ion mobility spectrometry (MALDI-2-TIMS-MSI) to map the spatial distribution of over 30 different sterol species in sections of the symbiotic system. The use of laser post-ionization crucially increased ion yields and allowed the recording of images with a pixel size of 5 μm. Trapped ion mobility spectrometry (TIMS) helped with the tentative assignment of over 30 sterol species. Correlation with anatomical features of the worm, revealed by host-derived phospholipid signals, and the location of the dinoflagellates, revealed by chlorophyll a signal, disclosed peculiar differences in the distribution of different sterol species (e.g. of cholesterol versus stigmasterol) within the receiving host. These findings point to sterol species-specific roles in the metabolism of Waminoa beyond a mere source of energy. They also underline the value of the MALDI-2-TIMS-MSI method to future research in the spatially resolved analysis of sterols.

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Current advances in phytosterol free forms and esters: Classification, biosynthesis, chemistry, and detection

Khallouki,

Zennouhi,

Hajji

et al. 2024

Steroids

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Analysis of phytosterol by MALDI-TOF mass spectrometry

Cited by 3 publications

References 54 publications

Mass Spectrometric Approaches for the Analysis of Phytosterols in Biological Samples

Mass Spectrometric Approaches for the Analysis of Phytosterols in Biological Samples

Molecular insights into symbiosis—mapping sterols in a marine flatworm-algae-system using high spatial resolution MALDI-2-MS imaging with ion mobility separation

Current advances in phytosterol free forms and esters: Classification, biosynthesis, chemistry, and detection

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