Analysis of plasma catecholamines by liquid chromatography with amperometric detection using a novel SPE ion‐exchange procedure

Abstract

Analysis of plasma catecholamines by liquid chromatography with amperometric detection using a novel SPE ion-exchange procedure Simultaneous determination of the three main catecholamines (Epinephrine, Norepinephrine, and Dopamine) has been implemented by means of liquid chromatography with amperometric detector working in oxidation at +800 mV. The chromatographic analysis was carried out on a reversed phase column (C8 Rainin 15064.6 mm ID, 5 lm) with a mixture of methanol and an aqueous solution of 5 mM of citric acid, 20 mg L -1 octanesulfonic acid, and 20 mg L -1 EDTA (pH 2.9) as the mobile phase. An original solid phase extraction pretreatment of plasma samples was developed using cationic ionic exchange cartridges with carboxypropylic adsorbent. The steps of the SPE procedure were carefully checked and optimal elution was carried out with perchloric acid. High extraction yield values were obtained for all catecholamines with good reproducibility. Linearity was observed in the 0.06 -3.00 ng mL -1 concentration range for all analytes. LOD and LOQ were respectively 0.03 ng mL -1 and 0.06 ng mL -1 . The method was applied successfully to the analysis of some real plasma samples from young volunteers under experimental psychological stress.

Biogenic amines and their metabolites in mouse brain tissue: Development, optimization and validation of an analytical HPLC method

“…The use of 10-kDa-protein cut-off filters to eliminate mainly enzymes involved in the degradation of the biogenic amines and their metabolites (e.g., MAO enzymes), has clearly increased the stability of these molecules and reduced the time of analysis. Our proposed method for sample preparation allowed direct injection of a clean biological sample without employing the complex extraction techniques, such as liquid/liquid extraction [49][50][51][52], adoption on aluminum oxide [28,29,[53][54][55][56][57][58], or solid phase extraction using ion-exchange [58][59][60][61], which previously have been used to extract trace amounts of the endogenous molecules in brain tissues. Consequently, our proposed method reduces laborious work for sample preparation, increases the reliability of the results, is simple, quick, inexpensive and satisfies all criteria for a good biological sample preparation.…”

Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism

“…Liquid chromatography (LC) with electrochemical detection is traditionally used for the analysis of this class of compounds due to its excellent detection limits [30][31][32]. However, a serious limitation of this technique is its inability to positively identify the detected compounds in a complex biological extract.…”

Selective extraction and analysis of catecholamines in rat blood microdialysate by polymeric ionic liquid-diphenylboric acid-packed capillary column and fast separation in high-performance liquid chromatography-electrochemical detector

“…SPE has been rapidly developed for pretreatment and extraction because it is more sensitive, environmentally friendly, faster and conserves sample [13]. Several different SPE sorbents have been reported to extract CAs from biological samples, including crown ether-modified polymers [12], calixarene [14], boronate compounds [15,16], ion exchange resin [17,18], C18 matrix [19] and alumina [20,21]. An advanced 96-well microplate extraction method using packed activated aluminum oxide was reported to decrease the plasma catecholamine analysis time and offer higher analytical specificity.…”