Biogenic amines and their metabolites in mouse brain tissue: Development, optimization and validation of an analytical HPLC method

Nguyen, A.T.; Aerts, Tony; Dam, Debby Van; Deyn, Peter Paul De

doi:10.1016/j.jchromb.2010.09.019

Cited by 32 publications

(39 citation statements)

References 53 publications

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“…The found LOQs are similar to or better than the ones reported with other chromatographic methods using electrochemical detection, where LOQs of 3 nM up to 70 nM were obtained [9,10,25]. The LOQs observed in the current study were significantly better than LOQs observed with LC-MS/MS methods (app.…”

Section: Linearity and Loqsupporting

confidence: 87%

“…The obtained analytical run time of app. 25 min is comparable with run times observed in previous publications, where both MHPG and 5-HT have been quantified simultaneously [7,9].…”

Section: Primary Neuronal Cell Culturesupporting

confidence: 85%

“…For detection of the monoamines and their biogenic metabolites, three different detection principles are mostly applied: electrochemistry [4,[7][8][9][10][11], fluorescence [12,13] or mass spectrometry [14,15]. An advantage with electrochemical detection is that no derivatization of the analytes is required, since they are electrochemically active [10].…”

Section: Introductionmentioning

confidence: 99%

“…A challenge in separating the monoamines and their metabolites is the relatively large difference in polarity between the polar metabolite of NA, MHPG, and the more apolar 5-HT, possibly leading to long run times. Only a few methods exist, where MHPG has been simultaneously quantified with the parent monoamine neurotransmitters despite the important role of MHPG in evaluating neurochemical imbalances of the noradrenergic system [7,9,16]. Some of the recently described methods for determination of monoamine neurotransmitters involve the use of instrumentation or cells specifically designed for monoamine analysis [4,9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Only a few methods exist, where MHPG has been simultaneously quantified with the parent monoamine neurotransmitters despite the important role of MHPG in evaluating neurochemical imbalances of the noradrenergic system [7,9,16]. Some of the recently described methods for determination of monoamine neurotransmitters involve the use of instrumentation or cells specifically designed for monoamine analysis [4,9,10]. In addition, quantification of neurotransmitters from primary neuronal cell cultures has only been scarcely described in the literature [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain

Schou-Pedersen

Hansen

Tveden‐Nyborg

et al. 2016

Journal of Chromatography B

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Section: Linearity and Loqsupporting

confidence: 87%

“…The obtained analytical run time of app. 25 min is comparable with run times observed in previous publications, where both MHPG and 5-HT have been quantified simultaneously [7,9].…”

Section: Primary Neuronal Cell Culturesupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain

Schou-Pedersen

Hansen

Tveden‐Nyborg

et al. 2016

Journal of Chromatography B

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An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Bidel

Corvaisier

Jozet‐Alves

et al. 2016

Biomedical Chromatography

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The cuttlefish belongs to the mollusk class Cephalopoda, considered as the most advanced marine invertebrates and thus widely used as models to study the biology of complex behaviors and cognition, as well as their related neurochemical mechanisms. Surprisingly, methods to quantify the biogenic monoamines and their metabolites in cuttlefish brain remain sparse and measure a limited number of analytes. This work aims to validate an HPLC-ECD method for the simultaneous quantification of dopamine, serotonin, norepinephrine and their main metabolites in cuttlefish brain. In comparison and in order to develop a method suitable to answer both ecological and biomedical questions, the validation was also carried out on a phylogenetically remote species: mouse (mammals). The method was shown to be accurate, precise, selective, repeatable and sensitive over a wide range of concentrations for 5-hydroxyindole-3-acetic acid, serotonin, dopamine, 3,4-dihydroxyphenylacetic acid and norepinephrine in the both extracts of cuttlefish and mouse brain, though with low precision and recovery for 4-hydroxy-3-methoxyphenylethylene glycol. Homovanillic acid, accurately studied in rodents, was not detectable in the brain of cuttlefish. Overall, we described here the first fully validated HPLC method for the routine measurement of both monoamines and metabolites in cuttlefish brain. Copyright © 2016 John Wiley & Sons, Ltd.

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Abnormal levels of seven amino neurotransmitters in depressed rat brain and determination by HPLC‐FLD

Cui

Qiu

Huang

et al. 2017

Biomedical Chromatography

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The determination of amino acids with actions like neurotransmitters or modulators has been increasingly important for diagnosis in many neuropsychiatric diseases. A rapid and simple high-performance liquid chromatography with fluorescence detection method was developed for simultaneous determination of seven amino acids: aspartate (Asp), glutamate (Glu), serine (Ser), glutamine (Gln), glycine (Gly), taurine (Tau) and γ-aminobutyric` acid (GABA). Homoserine was used as an internal standard. The analysis was performed on a BDS column with methanol and 50 mm sodium acetate solution (pH 6.5) using a simple gradient elution. Several parameters of the developed method were validated including linearity, accuracy, precision, extraction recovery and stability, which were within the acceptable range. The method was successfully applied to determination of real samples: hippocampus and cortex in depressed rats exposed to chronically unpredictable stress in order to study if there existed differences in the seven amino acids levels between depressed rats and control. The results showed that Asp, Gly, Tau and GABA significantly decreased with increasing Gln in the hippocampus of depressed rats, compared with that of the control group, among which obviously lower level of Asp and higher level of Gln in cortex were observed. The analytical method and the results could be useful for clinical diagnosis and further insight into pathophysiological mechanism of depression.

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Biogenic amines and their metabolites in mouse brain tissue: Development, optimization and validation of an analytical HPLC method

Cited by 32 publications

References 53 publications

Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain

Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Abnormal levels of seven amino neurotransmitters in depressed rat brain and determination by HPLC‐FLD

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