Analysis of polyamines as markers of (patho)physiological conditions

Teti, Diana; Visalli, Maria; McNair, Harold M.

doi:10.1016/s1570-0232(02)00669-4

Cited by 115 publications

(78 citation statements)

References 276 publications

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“…21,22) Moreover, we believe that the measurement of ODC is of little relevance to the current study considering its short half-life, which is less than 20 min. 1,23) Second, the statistical power of such a small sample size might be the cause of some concern. In addition, the randomization for subject selection to reflect balanced genotype proportions as used in this study did not represent the population genotype distribution, which might hinder the generalization of the results.…”

Section: Discussionmentioning

confidence: 99%

“…1) The key enzyme in mammalian polyamine biosynthesis is ornithine decarboxylase (ODC) which catalyzes the formation of putrescine, the precursor of spermidine and spermine, via decarboxylation of ornithine usually produced by arginase. [1][2][3] In humans, the ODC gene is known to have a restriction fragment length polymorphism when cleaved with Pst I (Hickok), and two ODC alleles as A or G were * To whom correspondence should be addressed: Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20, Daikominami, Higashi-ku, Nagoya 461-8673, Japan. Tel.…”

Section: Introductionmentioning

confidence: 99%

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Association of a Polymorphism in the Ornithine Decarboxylase Gene with Whole Blood Polyamine Concentrations in a Non-smoking Healthy Population

Kondo

Hamajima

Nishio

et al. 2007

JOURNAL OF HEALTH SCIENCE

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Ornithine decarboxylase (ODC) is the rate-limiting key enzyme in the biosynthesis of polyamines which have been confirmed to possess potent antioxidant and antiglycating properties. The aim of the present study was to examine the relationship of the ODC polymorphism and circulating whole blood polyamine concentrations. The study subjects were non-smoking, healthy men (n = 30) and women (n = 30) aged 55-69 years with equal numbers of AA, GA, and GG genotypes of the ODC gene, who were randomly recruited from 607 health checkup examinees. The whole blood polyamines (spermidine and spermine) were determined by high-performance liquid chromatography and were adjusted for hematocrit. The difference in the adjusted polyamines across the ODC gene was statistically tested using analysis of covariance. Women homozygous for the A-allele showed significantly higher spermidine levels than those with other genotypes. No such association was found among men, and spermine showed no association with the ODC genotype. In conclusion, the ODC polymorphism is significantly associated with whole blood polyamines in women. The ODC gene seems to be expressed more actively among female A-allele homozygotes than women carrying the G-allele.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of a Polymorphism in the Ornithine Decarboxylase Gene with Whole Blood Polyamine Concentrations in a Non-smoking Healthy Population

Kondo

Hamajima

Nishio

et al. 2007

JOURNAL OF HEALTH SCIENCE

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“…15 N-Labeled polyamines, [1,[4][5][6][7][8][9][10][11][12][13][14][15] N 2 ]putrescine ( 15 N-put), [1,4,[8][9][10][11][12][13][14][15] N 3 ]spd ( 15 N-spd) and [1,4,8,[12][13][14][15] N 4 ]spm ( 15 N-spm) were prepared as described previously. (3-aminopropyl)cyclohexylamine (APCHA), an inhibitor of spermine synthase, were purified by the respective recrystallization of the hydrochlorides of cis/trans-4-methylcyclohexylamine and N-(3-aminopropyl)-cyclohexylamine (Ͼ98%) 11) obtained from Tokyo Kasei Kogyo, Ltd. (Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Although a variety of analytical methods for polyamines have been developed, [5][6][7] there is still a need for a simpler, more sensitive and accurate method. The mass spectrometric method can simultaneously separate and identify polyamines on the basis of the difference of their mass without use of column.…”

mentioning

confidence: 99%

Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine

Samejima

Ôtani

Murakami

et al. 2007

Biological & Pharmaceutical Bulletin

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A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15 N-labeled polyamines.

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“…In our recent reports, altered metabolic patterns of free fatty acids (FFAs) 11 and polyamines (PAs) 12 as important biochemical indicators of pathological condition [13][14][15][16] were studied in rat plasma after injection with two adenoviruses, β-galactosidase expressing recombinant adenovirus (rAdLacZ) and RCA. FFA levels in the plasma of rAdLacZ and RCA rats were significantly different compared with those of control rats.…”

Section: Introductionmentioning

confidence: 99%

Altered Amino Acid Metabolic Patterns in the Plasma of Rat Models with Adenovirus Infection

Paik¹,

Shim²,

Moon³

et al. 2011

Bulletin of the Korean Chemical Society

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The presence of replication-competent adenovirus (RCA) subpopulations in adenoviral vector products raises a variety of safety issues for development of therapies based on gene therapy. To analyze the differing effects of adenoviral vector and RCA in vivo, we examined alterations in amino acids (AAs) using rat plasma following injection of β-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA. Plasma AAs were examined by gas chromatography-mass spectrometry. A total of 16 AAs were positively measured. In the rAdLacZ group compared to the control group, the level of aspartic acid was significantly increased (Student's t-test), while the level of glutamic acid was significantly reduced. Additionally, in the RCA group compared to the control group, the level of four AAs, valine, leucine, and isoleucine as branched-chain amino acids, and proline were significantly increased, whereas the levels of three AAs, glycine, threonine, and glutamic acid were significantly reduced. Altered plasma free AA metabolic patterns in rAdLacZ and RCA groups, compared with the control group, may explain the disturbance of AA metabolism related to viral infection.

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Analysis of polyamines as markers of (patho)physiological conditions

Cited by 115 publications

References 276 publications

Association of a Polymorphism in the Ornithine Decarboxylase Gene with Whole Blood Polyamine Concentrations in a Non-smoking Healthy Population

Association of a Polymorphism in the Ornithine Decarboxylase Gene with Whole Blood Polyamine Concentrations in a Non-smoking Healthy Population

Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine

Altered Amino Acid Metabolic Patterns in the Plasma of Rat Models with Adenovirus Infection

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