Analysis of prednisolone in plasma by high-performance liquid chromatography

Loo, J. C. K.; Butterfield, A.G.; Moffatt, James D.; Jordan, Nancy C.

doi:10.1016/s0378-4347(00)81807-1

Cited by 43 publications

(6 citation statements)

References 11 publications

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“…These have made use of high pressure liquid chromatography (Loo et al, 1977), thin layer chromatography coupled with fluorodensitometry (Morrison et aI., 1977), and gas-liquid chromatography with chemical ionisation mass spectrometry to overcome the lack of sensitivity, using selective derivatisation and solvent partitioning to remove interfering endogenous steroid (Matin and Amos, 1975).…”

Section: I Determination In Plasmaanalytical Techniquesmentioning

confidence: 99%

Clinical Pharmacokinetics of Prednisone and Prednisolone

Pickup

1979

Clinical Pharmacokinetics

169

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Prednisone and prednisolone are used in a wide variety of diseases. The two compounds are metabolically interconvertible; prednisolone is assumed to be the pharmacologically active species.Prednisone and prednisolone plasma concentrations are commonly determined by either radioimmunoassay or competitive protein binding techniques. No relationship has been demonstrated between prednisolone plasma concentration (unbound or total concentration) and clinical response; alternate day dosage regimens with fluctuating plasma concentrations being considered generally to be as effective with less side effects than a daily dosage regimen.Both drugs are rapidly absorbed after oral administration, reaching peak plasma concentrations after I to 3 hours. In general, plasma half-lives for prednisone are slightly longer (3.4 to 3.8h) than for prednisolone (2.1 to 3.5h). Either drug can be prescribed in most situations. On average, however, the bioavailability of prednisolone after oral prednisone is approximately 80 % of that after prednisolone. A wide intersubject variation in prednisolone concentration is evident after both drugs, which may suggest impaired drug absorption in some individuals. Prednisolone shows dose dependent pharmacokinetics; an increase in dose leading to an increase in volume of distribution and plasma clearance. This can be explained in terms of the non-linear binding of the drug to plasma proteins. The degree of binding will determine the distribution and clearance of free (i.e. pharmacologically active) drug. Reduced doses are recommended in patients with hypoalbuminaemia.Prednisolone pharmacokinetics are also dependent on age; the half-life being shorter in children. Liver disease prolongs the prednisolone half-life and, due to thefrequently associated hypoalbuminaemia, also increases the percentage of unbound drug. It has been recommended by some that prednisolone rather than prednisone is the drug of choice in active liver disease owing to the poor conversion of prednisone to prednisolone in some patients. However, the reduced plasma concentration of prednisolone in such patients is compensated for by delayed clearance. Thus, there is little advantage of one preparation over the other.

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Section: I Determination In Plasmaanalytical Techniquesmentioning

confidence: 99%

Clinical Pharmacokinetics of Prednisone and Prednisolone

Pickup

1979

Clinical Pharmacokinetics

169

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“…1), analytical methods must be selective enough to distinguish between the synthetic and the endogenous corticosteroids. Previously reported analytical procedures for the determination of these compounds, either simultaneously or individually in plasma or serum, have employed a variety of techniques such as radioimmunological or competitive protein binding principles [6,7], micellar electrokinetic capillary chromatography (MECC) [8], high performance liquid chromatography (HPLC) with UV detection [9][10][11][12], fluorescence detection [13] or diode array detection coupled with second-order calibration [14], gas chromatography-stable isotope dilution mass spectrometry [15], gas chromatography-mass spectrometry (GC-MS) after purification by immunoaffinity chromatography [16] and liquid chromatography-mass spectrometry or tandem mass spectrometry [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Development of a sensitive and selective method for the quantitative analysis of cortisol, cortisone, prednisolone and prednisone in human plasma

Ionita

Fast

Akhlaghi

2009

Journal of Chromatography B

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“…The specificity of the assays was confirmed by high performance liquid chromatographic procedure. (Loo, Butterfield & others 1977;Loo &Jordan, 1977).…”

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confidence: 99%