Analysis of related substances in bisoprolol fumarate on sub-2-μm adsorbents

Abstract

Summary.The new analytical method of very high pressure liquid chromatography (VHPLC) has been designed for the estimation of related substances in bisoprolol fumarate. Several columns filled with sub-2-μm totally porous and shell adsorbent have been tested and compared during the method development. The columns' resolution was investigated. The shell adsorbent appears to have smaller resolution than the totally porous adsorbent. The tips and recommendations for the HPLC-VHPLC method transfer are presented. Significant reduction in analysis run time and solvent consumption has been obtained.

Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects

“…UHPLC provides faster separations and lower solvent consumption compared to HPLC, with preserved column efficiency [2,3]. This is achieved by decreasing the particle size of the stationary phase and increasing the linear velocity of the mobile phase.…”

Optimization of HILIC method for the analysis of bisoprolol and its impurities considering uncertainties in peak position

“…Also, fully validated simultaneous determination of bisoprolol fumarate and hydrochlorothiazide in their dosage forms using different voltammetric, chromatographic, and spectrophotometric analytical methods was developed [18].The method for the investigation of bisoprolol and impurities is included in European Pharmacopeia 7 [19]. Several papers dealing with HPLC determination of bisoprolol and potential impurities simultaneously were found [20,21]. This paper would be the first one in which HILIC method is applied for the determination of bisoprololfumarate and its impurities.…”