Analysis of Rutin in the Extract and Gel of Viola tricolor

Piana, Mariana; Zadra, Marina; Brum, Thiele Faccim de; Boligon, Aline Augusti; Gonçalves, Adiene Fernandes Kieling; Cruz, Ritiel Corrêa da; Freitas, Robson Borba de; Canto, Gizele Scotti do; Athayde, Margareth Linde

doi:10.1093/chromsci/bms155

Cited by 20 publications

(5 citation statements)

References 13 publications

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“…The rutin content for the saline extract and fraction was 0.53 ± 0.0010% and 0.24 ± 0.0033%, respectively. Our results are similar to studies with BmoLL conjugated with quantum dots [ 20 ] which showed an HA of 1024 in hemagglutination tests, as well as in studies with extract of Viola tricolor [ 27 ] and ethanol extract of Caesalpinia pyramidalis [ 28 ] regarding the presence of this metabolites.…”

Section: Resultssupporting

confidence: 90%

Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

et al. 2022

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Biomphalaria glabrata snails constitute the main vector of schistosomiasis in Brazil, and Bauhinia monandra Kurz, the leaves of which contain BmoLL lectin with biocidal action, is a plant widely found on continents in which the disease is endemic. This work describes the composition of B. monandra preparations and the effect on embryos and adult snails, their reproduction parameters and hemocytes. We also describe the results of a comet assay after B. glabrata exposure to sublethal concentrations of the preparations. Additionally, the effects of the preparations on S. mansoni cercariae and environmental monitoring with Artemia salina are described. In the chemical evaluation, cinnamic, flavonoid and saponin derivatives were detected in the two preparations assessed, namely the saline extract and the fraction. Both preparations were toxic to embryos in the blastula, gastrula, trochophore, veliger and hippo stages (LC50 of 0.042 and 0.0478; 0.0417 and 0.0419; 0.0897 and 0.1582; 0.3734 and 0.0974; 0.397 and 0.0970 mg/mL, respectively) and to adult snails (LC50 of 6.6 and 0.87 mg/mL, respectively), which were reproductively affected with decreased egg deposition. In blood cell analysis, characteristic cells for apoptosis, micronucleus and binucleation were detected, while for comet analysis, different degrees of nuclear damage were detected. The fraction was able to cause total mortality of the cercariae and did not present environmental toxicity. Therefore, B. monandra preparations are promising in combating schistosomiasis since they can control both the intermediate host and eliminate the infectious agent, besides being safe to the environment.

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Section: Resultssupporting

confidence: 90%

Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

et al. 2022

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“…41 HPLC is a versatile, robust, and widely used technique used in phytochemical and analytical chemistry for quantification, identification and purification of the mixture components. 42 Moreover this technique is also useful for characterization and quantification of secondary metabolites in plant extract. 43 HPLC studies revealed that concentration of lupeol in Pyrus pashia ethanolic exract was 4.24% w/w.…”

Section: Discussionmentioning

confidence: 99%

Quality Control standardization of Wild Himalayan Pear: Pyrus pashia

Hemalatha¹,

Sharma²,

Prasad³

2016

Phcogj

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Introduction: To establish the pharmacognostical and phytochemical standardization parameters of Pyrus pashia fruits in order to ensure quality and safety of this traditionally acclaimed medicinal tree. Methods: The fresh fruits of P. pashia were collected and dried. Fruit was subjected to various pharmacognostical investigations, Extraction procedures, and preliminary phytochemical screening, according to WHO guidelines. Ethanolic extract was standardized to total phenolic and flavonoid content, followed by phyto chemical quantification of P. pashia extract using lupeol as a chemical marker by HPLC method. Results: In the present study, microscopy of the fruit showed typical characteristics of berry, having thick fleshy pericarp differentiated into thin epicarp and thick mesocarp having wide radiating carpel chambers with one or two seeds attached in axile placentum. Further, physicochemical evaluation was done like, loss on drying, total ash value, acid insoluble ash value, water soluble ash value, fluorescence analysis etc. Heavy metal and pesticide residue analysis was also performed. Furthermore, ethanolic extract of Pyrus pashia (EPP) obtained from cold maceration and phytochemical screening of different fractions obtained by

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“…It is used in analytical chemistry and phytochemistry to separate a mixture of substances. HPLC is a robust, dependable, and widely used method for the isolation of natural substances [40][41]. For measuring and characterizing secondary metabolites in plant extracts, such as phenol compounds, flavonoids, alkaloids, steroids, and other steroid-like substances, HPLC has been advocated in various articles [42][43][44][45][46].…”

Section: Hplcmentioning

confidence: 99%

A review of extraction and HPLC methods used for Melastomataceae and Asteraceae leaves in the analysis of flavonoids

Binti

Hamid

Latip

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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HPLC (high-performance liquid chromatography) is one of the accurate chromatographic techniques that is widely used for analysis. This present review submitted the extraction and HPLC methods for determining the compound in Melastomataceae and Asteraceae families that related with insecticidal compounds. This review discusses the standard used for the compound and chromatographic conditions. There are several species under both families. As for the Melastomataceae family, the species are Melastoma malabathricum and Miconia albicans meanwhile, the species under the Asteraceae family are Santolina semidentata, Eupatorium littorale, and Vernonia condensata. This review focus on the flavonoid and phenolic compound as these compounds are categorized as an insecticidal compound. HPLC can be used in conjunction with several detection technologies to identify flavonoids and phenolics in the plant, food, and biological samples because it is a method for separating these molecules. As a result, the HPLC procedure for the chosen molecule was reviewed in this article, which will aid in further analysis.

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Analysis of Rutin in the Extract and Gel of Viola tricolor

Cited by 20 publications

References 13 publications

Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

Quality Control standardization of Wild Himalayan Pear: Pyrus pashia

A review of extraction and HPLC methods used for Melastomataceae and Asteraceae leaves in the analysis of flavonoids

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