Analysis of six active components in Radix tinosporae by nonaqueous capillary electrophoresis with mass spectrometry

Abstract

Nonaqueous capillary electrophoresis with mass spectrometry has advantages for the analysis of active components in herbs. Here, a rapid nonaqueous capillary electrophoresis with mass spectrometry method was developed to separate, identify, and quantify palmatin, columbin, cepharanthine, menisperine, magnoflorine, and 20-hydroxyecdysone in Radix tinosporae. Electrospray ionization MS spectra of the six components were collected and possible cleavage pathways of main fragment ions were elucidated. The conditions that could affect separation, such as the composition of running buffer and applied voltage, were studied, and the conditions that could affect the mass spectrometry detection, such as the composition and flow rate of sheath liquid, the pressure of nitrogen gas, and the temperature and flow rate of the dry gas, were also optimized. Under the optimized conditions, the correlation coefficient was >0.99. The relative standard deviations of migration time and peak areas were <10%. The recoveries were calculated to be 99.31-107.80% in real samples. It has been demonstrated that the proposed method has good potential to be applied to determine the six bioactive components in Radix tinosporae.

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

“…Liu et al. analyzed active ingredients from Radix tinosporae and characterized several compounds potentially being responsible for its anti‐inflammatory or anti‐viral effects . NACE‐MS based on ammonium acetate in ACN‐methanol mixtures was used to separate a total of 6 relevant compounds.…”

Cepharanthine: An update of its mode of action, pharmacological properties and medical applications

“…The principle of pharmacovigilance applied to herbal medicinal products (or phytovigilance) is now well taken in account by the international health agencies (Lehmann and Pabst, 2016). CEP has also been detected in Radix Tinosporae, an herb used in traditional Chinese medicine for the treatment of various inflammatory diseases (Xiang et al, 2016;Liu et al, 2017). This is perhaps not entirely surprising because Tinosporae Radix is the dried root tuber of Tinospora sagittata (Oliv.)…”

Capillary electrophoresis in phytochemical analysis (2014–2017)

“…Although UV had been the most frequently used detection method in CE, MS was also widely used due to the high sensitivity and selectivity. Few examples are determination of mushroom toxins ibotenic acid, muscimol and muscarine in human urine , fingerprinting analysis of flavonoids in Ginkgo biloba herbal supplements , and fast determination of harmala alkaloids in edible algae by CE‐MS; Differential detection of Rhizoma coptidis by CE‐MS with a nanospray interface ; Characterization of hydrothermally isolated xylan from beech wood by CE‐laser‐induced fluorescence (LIF) and MS detection ; Analysis of paspalic acid, lysergic acid, and iso‐lysergic acid with UV and Q‐TOF/MS detection ; Dereplication of known nucleobase and nucleoside compounds in natural product extracts by CE‐high resolution mass spectrometry (HR‐MS) ; Separating and determining physcion, chrysophanol and aloe‐emodin in rhubarb and quantifying palmatin, columbin, cepharanthine, menisperine, magnoflorine and 20‐hydroxyecdysone in Radix tinosporae by NACE‐MS.…”