ANALYSIS OF TAXINES IN <i>TAXUS</i> PLANT MATERIAL AND CELL CULTURES BY HPLC PHOTODIODE ARRAY AND HPLC-ELECTROSPRAY MASS SPECTROMETRY

Theodoridis, Georgios; Laskaris, Gregory; Rozendaal, Erik L. M. van; Verpoorte, Robert

doi:10.1081/jlc-100105139

Cited by 14 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…capillary electrophoresis [15], LC-MS [16] and HPLC [17,18]. A critical literature survey reveals at this time that RP HPLC may be an overwhelming choice for its analysis.…”

Section: Introductionmentioning

confidence: 99%

HPLC method for the determination of carboplatin and paclitaxel with cremophorEL in an amphiphilic polymer matrix

Mittal

Kumar

2007

Journal of Chromatography B

View full text Add to dashboard Cite

“…capillary electrophoresis [15], LC-MS [16] and HPLC [17,18]. A critical literature survey reveals at this time that RP HPLC may be an overwhelming choice for its analysis.…”

Section: Introductionmentioning

confidence: 99%

HPLC method for the determination of carboplatin and paclitaxel with cremophorEL in an amphiphilic polymer matrix

Mittal

Kumar

2007

Journal of Chromatography B

View full text Add to dashboard Cite

“…Chromatograms were recorded in the full scan mode at 200-400 nm (total runtime 90 min) and evaluated at 280 nm (Theodoridis et al 2001). A few different setups were tried.…”

Section: Hplcmentioning

confidence: 99%

“…All parts of the tree are toxic except for the red aril covering the seed (Shanker et al 2002;Wilson et al 2001). The toxicity of the different Taxus species varies, the pacific yew being less toxic than the European yew (Theodoridis et al 2001;Van Rozendaal et al 1998;Wilson et al 2001). Also differences exist between the different T. baccata subspecies regarding the content of paclitaxel and related taxoids (Van Rozendaal et al 2000).…”

Section: Introductionmentioning

confidence: 96%

Yew intoxication in brown bears; a novel approach to diagnosis

et al. 2010

View full text Add to dashboard Cite

Tissues from two juvenile brown bears (Ursus arctos), suspected to have died from yew (Taxus baccata) toxicosis, were chemically examined using thin layer chromatography and high performance liquid chromatography. Extraction and analysis were conducted on heart and liver tissue samples as well as stomach content from the two bears and also on fresh material from an authenticated yew tree which was used as a standard for comparison. The taxine complex comprised of taxine B, 1-deoxytaxine B, isotaxine B, 2-acetyltaxin B, 1-deoxyisotaxine B, 2-acetylisotaxine B, and cinnamates were detected in all extracted samples. taxine B and its isomer isotaxine B are the main toxic constituents in yew. The heart, the target organ of taxine B, is arrested when reaching the lethal tissue concentration of taxine complex. In the present cases, the concentration in the hearts was found to be 37 and 17 μg taxine complex per gram tissue, respectively. As no further absorption into the heart occurs following cardiac arrest, the concentration determined is the actual lethal tissue concentration. Yew ingestion was confirmed by microscopic examination of stomach contents of both bears.Histopathological findings of contraction band necrosis in heart specimens were also consistent with yew intoxication.

show abstract

“…Many methods for the analysis of the manifold Taxus ingredients have been described: immunoassay [23][24][25], thin-layer chromatography [15,26,27], gas chromatography (GC) [10], and GC/mass spectroscopy (MS) [1,15,18,28], as well as high-performance liquid chromatography (HPLC) combined with UV [15,25,[29][30][31][32][33][34] and MS [13,14,17,35,36]. However, most of the papers deal with the detection of extracts of Taxus spp, whereas analytical procedures of forensic relevance were seldom reported.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of five fatal cases of Taxus poisoning

et al. 2006

View full text Add to dashboard Cite

The study presents five fatal cases of poisoning with Taxus spp., all of which were suicides of young people aged between 16 and 26 years. Yew leaves were consumed in four fatalities, whereas a mash from Taxus was ingested in one case. No relevant concentrations of alcohol, narcotic drugs, and pharmaceuticals were determined in postmortem toxicological screening. At forensic autopsy, a widely dilated pupil was found in two decedents. Furthermore, autopsy showed unspecific findings of intoxication in all cases: acute blood congestion of lungs, liver, kidney, and brain as well as dilated cardiac ventricles. No signs of violence could be found in any of the fatalities. Yew leaves were identified in four cases in the stomach and duodenum. 3,5-Dimethoxyphenol, the aglycon of the Taxus ingredient taxicatine, was determined as toxicological evidence for the absorption of yew ingredients. Taxus intoxication could be confirmed by 3,5-dimethoxyphenol concentrations in cardiac blood between 31 and 528 ng/ml for all cases. 3,5-Dimethoxyphenol was also detected in stomach contents as well as in urine, liver, kidneys, and brain samples. Based on the different concentrations of 3,5-dimethoxyphenol determined in the cardiac blood samples, it was concluded that the form of ingestion plays a decisive role in the process of poisoning. Finally, a toxic range for Taxus poisoning based on 3,5-dimethoxyphenol as marker substance is proposed as orientation.

show abstract

ANALYSIS OF TAXINES IN TAXUS PLANT MATERIAL AND CELL CULTURES BY HPLC PHOTODIODE ARRAY AND HPLC-ELECTROSPRAY MASS SPECTROMETRY

Cited by 14 publications

References 20 publications

HPLC method for the determination of carboplatin and paclitaxel with cremophorEL in an amphiphilic polymer matrix

HPLC method for the determination of carboplatin and paclitaxel with cremophorEL in an amphiphilic polymer matrix

Yew intoxication in brown bears; a novel approach to diagnosis

A comparative study of five fatal cases of Taxus poisoning

Contact Info

Product

Resources

About