Analysis of the behavior of 2D monolayers and 3D spheroid human pancreatic beta cells derived from induced pluripotent stem cells in a microfluidic environment

Analysis of the transcriptome and metabolome of pancreatic spheroids derived from human induced pluripotent stem cells and matured in an organ-on-a-chip

“…37 Recently, we have shown that hiPSC-derived b-cells pseudo-islets cultured in microfluidic biochips display the functions and markers of endocrine pancreas after 2 weeks of culture. 27 In the present study, we confirmed that the spheroids expressed insulin and glucagon (immunostaining) and typical pancreatic genes such as PDX1, NKX6.1, PTF1A, INS, NKX2.2 and GCK. Biomechanical and biochemical stimulations (through shear stress, cell interactions, and soluble factor concentrations) largely impact pancreatic islet function and responses.…”

“…The 3D honeycomb technology was developed by Shinohara et al, and has also been described in detail in previous works. 27,28 Before cell seeding, the honeycomb plates were sterilized with ethanol (1 hour) and coated with pluronic acid (Pluronic s F-127 Sigma, 10 mg mL À1 ) dissolved in phosphate-buffered saline (PBS, Gibco) overnight to prevent cell attachment. Then, the plates were rinsed three times with PBS and once with maintenance culture medium (bcell basal medium Y10104, supplemented with b-cell supplement Y10102).…”

An Immersible Microgripper for Pancreatic Islet and Organoid Research

“…While this preparation offers the potential advantage that the innervation is better preserved, it requires more specialized equipment and is less versatile than the microgripper, which cannot only be used for islet research, but also for research on virtually any organoid of spherical shape. This is a relevant feature for the emerging field of islet spheroid reaggregation, either from primary human cells, or from stem cells [ 25 , 26 , 27 ].…”

Design of the micropump and mass-transfer compartment of a microfluidic system for regular nonenzymatic glucose measurement

“…On the other side, using insulin injection or pills that induce insulin secretion are likely to result in an excessive decrease in the blood sugar level (hypoglycemia) in diabetic patients. As the normal performance of the brain heavily relies on glucose, hypoglycemia proves a real threat, as it may lead to dysfunction of the patient's brain or even put them into coma, in extreme cases [1] , [2] , [3] , [4] , [5] . Hence, diabetic patients need a regular measurement system to check their glucose level, which can be painlessly implanted.…”