2018
DOI: 10.1063/1.5031054
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Analysis of the dielectrophoretic properties of cells using the isomotive AC electric field

Abstract: Various microfluidic devices utilizing the principle of dielectrophoresis (DEP) have been developed to separate, concentrate, and characterize biological cells; however, their performance is still limited by a lack of quantitative characterization. We addressed this limitation by employing a method capable of accurately quantifying a cell's response to an imposed field gradient. In this study, a simple method using a newly designed Creek-gap electrode was proposed, and the electrokinetic behavior of cells was … Show more

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Cited by 10 publications
(11 citation statements)
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“…Development of the present insulator‐based dielectrophoretic (iDEP) device was motivated by the limited ability of the creek‐gap device with metal electrodes when the evaluation of Re( β ) across a wide range of frequency was required. Operation of the device with metal creek‐gap electrodes , using human breast epithelial cells (MCF10A) demonstrated that the value of Re( β ) at field frequencies, in which MCF10A cells exhibit n‐DEP, agreed relatively well with the analytically predicted values. However, measuring the velocimetry of cells exhibiting the p‐DEP effect is quite difficult because of the adherence of cells onto electrode edges.…”
supporting
confidence: 56%
See 1 more Smart Citation
“…Development of the present insulator‐based dielectrophoretic (iDEP) device was motivated by the limited ability of the creek‐gap device with metal electrodes when the evaluation of Re( β ) across a wide range of frequency was required. Operation of the device with metal creek‐gap electrodes , using human breast epithelial cells (MCF10A) demonstrated that the value of Re( β ) at field frequencies, in which MCF10A cells exhibit n‐DEP, agreed relatively well with the analytically predicted values. However, measuring the velocimetry of cells exhibiting the p‐DEP effect is quite difficult because of the adherence of cells onto electrode edges.…”
supporting
confidence: 56%
“…A. In order to produce a nonuniform electric field, E , in which the gradient of the field square (E2) in the channel direction (the positive direction of x ‐axis) takes a constant, the geometrical shape of the insulators was determined in a manner similar to the method utilizing an approximated solution of E2= constant obtained in the previous study . When a single spherical cell travels along the centerline of the channel of the device at a constant velocity, the cell experiences a constant DEP force, F DEP , the Stokes drag force, F d , and the friction force on the bottom face, F f .…”
mentioning
confidence: 99%
“…The concept of "iso-motive" DEP also rests within this category, with electrode configurations that generate a spatially uniform DEP force (hence "iso-motive") [28,29]. The approach counters one of the challenges to DEP-the non-uniform nature of the force-but all the above approaches lead to batch-mode separations, or continuous-flow separations that remain size-dependent.…”
Section: D Electrode Systems: Single-field/single-frequencymentioning
confidence: 99%
“…The primary advantage of isoDEP is that the response of a particle is uniform throughout the analytical space can be obtained via particle tracking; this concept is analogous to how particle rotation is used to measure particle dielectric properties through measuring their rotational speed [15,16]. Other designs have followed the premise of the isoDEP design and have been used successfully for single-cell analysis applications [17,18].…”
Section: Introductionmentioning
confidence: 99%