Analysis of the Exudate Produced by
            <i>Streptococcus mutans</i>
            SL-1 Colonies on Sucrose-Containing Agar Media

Donkersloot, Jacob A.; Leon, H. A.; Chassy, Bruce M.; Krichevsky, Micah I.

doi:10.1128/aem.32.3.448-450.1976

Appl Environ Microbiol

1976

DOI: 10.1128/aem.32.3.448-450.1976

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Analysis of the Exudate Produced by Streptococcus mutans SL-1 Colonies on Sucrose-Containing Agar Media

Jacob A. Donkersloot

H. A. Leon

Bruce M. Chassy

et al.

Abstract: The watery exudate produced by Streptococcus mutans SL-1 colonies on sucrose-containing agar media was found to contain about 7% (wt/vol) of a watersoluble, branched dextran, 4% sucrose, and smaller (less than 1%) amounts of fructose, Folin-phenol-positive material, and lactic acid.

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Clinical isolates of Streptococcus mutans serotype c with altered colony morphology due to fructan synthesis

Okahashi¹,

Asakawa²,

Kinoshita³

et al. 1984

Infect Immun

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Streptococcus mutans MT6801, MT6861, and MT6879, which form large mucoid colonies on mitis salivarius agar, were isolated from a mother and her two daughters. These isolates were identified as serotype c by immunodiffusion with serotype-specific antisera. The large colonies formed on sucrosecontaining agar were found to contain water-soluble fructan. The cell-free fructosyltransferase (FTase) activity of the strains which formed large colonies was five to eight times higher than that of serotype c S. mutans which produced small, rough colonies typical of this serotype. Furthermore, greater quantities of fructan were synthesized from sucrose by growing cells of MT6801 when compared with MT8148, a typical serotype c S. mutans. Glucosyltransferase and FTase could be isolated by chromatofocusing from culture supernatants of MT6801 and MT8148. The FTase activity of both strains was eluted at pH 4.5, and glucosyltransferase was released by elution with an NaCl linear gradient. The eluted FTase activity of MT6801 was significantly higher than that of MT8148. Strains MT6861 and MT6879 were also found to possess a similar property in terms of FTase activity. These results suggest that formation of large mucoid colonies by these strains is a consequence of high FTase activity.

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Clinical isolates of Streptococcus mutans serotype c with altered colony morphology due to fructan synthesis

Okahashi¹,

Asakawa²,

Kinoshita³

et al. 1984

Infect Immun

View full text Add to dashboard Cite

show abstract

3: Sweetening agents in food, drinks and medicine: Cariogenic potential and adverse effects

Db¹

1979

Int J Food Sci Nutr

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Analysis of the Exudate Produced by Streptococcus mutans SL-1 Colonies on Sucrose-Containing Agar Media

Abstract: The watery exudate produced by Streptococcus mutans SL-1 colonies on sucrose-containing agar media was found to contain about 7% (wt/vol) of a watersoluble, branched dextran, 4% sucrose, and smaller (less than 1%) amounts of fructose, Folin-phenol-positive material, and lactic acid.

Cited by 2 publications

References 16 publications

Clinical isolates of Streptococcus mutans serotype c with altered colony morphology due to fructan synthesis

Clinical isolates of Streptococcus mutans serotype c with altered colony morphology due to fructan synthesis

3: Sweetening agents in food, drinks and medicine: Cariogenic potential and adverse effects

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