Analysis of the oxidized low density lipoprotein receptor 1 gene as a potential marker for carcass quality traits in Qinchuan cattle

Gui, Linsheng; Raza, Sayed Haidar Abbas; Jia, Jianlei

doi:10.5713/ajas.18.0079

Cited by 14 publications

(3 citation statements)

References 25 publications

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“…The mean of body conformation traits was calculated using the Bonferroni method in the SPSS 24.0 (IBM Company, NY, USA) software package. The association between SNP markers, haplotype combinations, and body conformation traits was analyzed using a general linear model (GLM) with Bonferroni method as follows: yijk = u + gi + aj + sk + eijk [16] Where yijk is the phenotypic observation, u is the population mean, gi is the fixed effect of animal genotype, aj is the fixed effect of age, sk is the random effect of sire, and eijk is the residual error. Finally, a Bonferroni correction was performed to determine the p-value.…”

Section: A C C E P T E Dmentioning

confidence: 99%

Genetic variants of the growth differentiation factor 8 affect body conformation traits in Chinese Dabieshan cattle

et al. 2022

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Objective: The growth differentiation factor 8 (GDF8) gene plays a key role in bone formation, resorption, and skeletal muscle development in mammals. Here, we studied the genetic variants of GDF8 and their contribution to body conformation traits in Chinese Dabieshan cattle.Methods: Single nucleotide polymorphisms were identified in the bovine GDF8 gene by DNA sequencing. Phylogenetic analysis, motif analysis, and genetic diversity analysis were conducted using bioinformatics software. Association analysis between five SNPs, haplotype combinations, and body conformation traits was conducted in 380 individuals.Results: GDF8 was highly conserved in seven species, and the GDF8 sequence of cattle was most similar to the sequences of sheep and goat based on the phylogenetic analysis. The motif analysis showed that there were 12 significant motifs in GDF8. Genetic diversity analysis indicated that the polymorphism information content of the five studied SNPs was within 0.25 to 0.5. Haplotype analysis revealed that a total of 12 different haplotypes and those with a frequency of < 0.05 were excluded. Linkage disequilibrium analysis showed that there was a strong linkage (r 2 > 0.330) between the following SNPs: g.5070C > A, g.5076T > C, and g.5148A > C. Association analysis indicated that these five SNPs were associated with some of the body conformation traits (p < 0.05), and the animals with haplotype combination H1H1 (-GGGGCCTTAA-) had greater wither height, hip height, heart girth, abdominal girth, and pin bone width than the other animals (p < 0.05) in Dabieshan cattle. Conclusion:Overall, our results indicate that the genetic variants of GDF8 affected the body conformation traits of Chinese Dabieshan cattle, and the GDF8 gene could make a strong candidate gene in Dabieshan cattle breeding programs.

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Section: A C C E P T E Dmentioning

confidence: 99%

Genetic variants of the growth differentiation factor 8 affect body conformation traits in Chinese Dabieshan cattle

et al. 2022

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“…Kowalewska-Łuczak (2018) reported relatedness of the OLR1 gene polymorphisms with reproductive performance in dairy cattle. Relatedness of the OLR1 gene polymorphisms with growth and carcass traits in beef cattle has also been indicated (Fonseca et al 2015;Gui et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Single nucleotide polymorphisms and metabolic biochemical profile of productive markers characterize three European breeds of dairy cattle

et al. 2022

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The objective of this study was to investigate polymorphisms of DGAT1, FABP, OLR1 and ATP1A1 genes using PCR-DNA sequencing, and to associate these genetic structures to changes in metabolic biochemical markers and milk composition indicators in a total of 90 dairy cows of the Holstein, Simmental, and Brown Swiss breeds (30 cows each). PCR was carried out for amplification of 411-bp of DGAT1, 525-bp of FABP, 582-bp of OLR1, and 300-bp of ATP1A1 genes. Three breeds’ nucleotide sequence variations in the form of single nucleotide polymorphisms (SNPs) were detailed by DNA sequencing analysis. Chisquare analysis showed that the distribution of all discovered SNPs varied significantly (P < 0.001). Biochemical indices in cow’s serum revealed no significant difference in serum total protein, albumin, and total cholesterol among the three breeds. However, triglyceride showed a significant increase in Simmental compared to either Holsteins or Brown Swiss, while the highest mean value of triiodothyronine (T3) and tetraiodothyronine (T4) was detected in Holstein dairy cows The milk composition indicators analysis revealed that milk protein, sugar, and density were significantly higher in Holsteins than both Simmental and Brown Swiss. Meanwhile, milk fat and total solids revealed a significantly higher increase in Simmental than both brown Swiss and Holstein. As a result, the metabolic biochemical markers profile along with the identified SNPs could be used as a candidate and a reference guide for effective characterization of the Holstein, Simmental, and Brown Swiss breeds, leading to the creation of a marker-assisted selection system for production traits in dairy cattle breeds.

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“…It is regulated by the synergistic action of transcription factors (TFs) and regulatory proteins, and plays an important role in the accuracy and diversity of the transmission of genetic information [25,[33][34][35]. Transcription factor ACSL1 belong to the long-chain adipocyte-acid CoA family, which can catalyze the conversion of long-chain adipocyte acids into acyl coenzyme A in active form, which is used to synthesize cell lipids and is degraded by β-oxidation [36][37][38][39][40]. In addition, PPARα participates in lipid metabolism and inflammatory reaction [41].…”

Section: Discussionmentioning

confidence: 99%

The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region

Liang

Raza

et al. 2019

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The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based on the FAM13A amino acid sequence. This showed that subcutaneous adipose tissue had the highest expression in all tissues except lung tissue. Then we summarized the gene structure. The promoter region sequence of the gene was successfully amplified, and the −241/+54 region has been identified as the core promoter region. The core promoter region was determined by the unidirectional deletion of the 5’ flanking promoter region of the FAM13A gene. Based on the bioinformatics analysis, we examined the dual luciferase activity of the vector constructed by the mutation site, and the transcription factors ACSL1 and ASCL2 were found as transcriptional regulators of FAM13A. Moreover, electrophoretic mobility shift assay (EMSA) further validated the regulatory role of ACSL1 and ASCL2 in the regulation of FAM13A. ACSL1 and ASCL2 were finally identified as activating transcription factors. Our results provide a basis for the function of the FAM13A gene in bovine adipocytes in order to improve the deposition of fat deposition in beef cattle muscle.

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Analysis of the oxidized low density lipoprotein receptor 1 gene as a potential marker for carcass quality traits in Qinchuan cattle

Abstract: Based on our results, we believe that the OLR1 gene could be a strong candidate gene for influencing carcass quality traits in Qinchuan cattle.

Cited by 14 publications

References 25 publications

Genetic variants of the growth differentiation factor 8 affect body conformation traits in Chinese Dabieshan cattle

Genetic variants of the growth differentiation factor 8 affect body conformation traits in Chinese Dabieshan cattle

Single nucleotide polymorphisms and metabolic biochemical profile of productive markers characterize three European breeds of dairy cattle

The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region

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