Analysis of the penetration profiles of topically applied substances by laser scanning microscopy

Martschick, Anja; Teichmann, A.; Richter, Heike; Schanzer, Sabine; Antoniou, C.; Sterry, Wolfram; Lademann, Juergen

doi:10.1002/lapl.200610117

Cited by 15 publications

(15 citation statements)

References 14 publications

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“…A comprehensive review of the optical principles has been published elsewhere [12,13]. The fluorescent mode of the LSM was used for the experiments, and contrast was achieved by the intradermal injection of a fluorescent dye (0.1% fluorescein in water) prior to each evaluation.…”

Section: Laser Scanning Microscopy (Lsm)mentioning

confidence: 99%

Determination of the thickness and structure of the skin barrier by in vivo laser scanning microscopy

et al. 2007

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Normal skin barrier function is an essential aspect of skin homeostasis and regeneration. Dynamic inflammatory, proliferative and neoplastic skin processes such as wound healing, psoriasis and contact dermatitis are associated with a significant disruption of the skin barrier. In recent years, there has been increasing interest in evaluating cosmetic and pharmacologic products for their ability to restore these protective properties. The gold standard for characterization of barrier function has been the measurement of the transepidermal water loss, however the disadvantage of this method is its interference with several endogenous and exogenous factors such as hydration, perspiration and topically applied substances. This study was aimed to test the clinical applicability of a fluorescence confocal laser scanning microscope (LSM) for a systematic morphologic analysis of the structure, integrity and thickness of the stratum corneum in 10 otherwise healthy volunteers. The influence of skin treatment with commercial moisturizing cream on skin barrier function was evaluated in serial non-invasive examinations. Our findings showed that in vivo LSM may represent a simple and efficient method for the characterization of skin barrier properties, such as the thickness and hydration of the stratum corneum. µmDetermination of the boundary between the SC and the SS

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Section: Laser Scanning Microscopy (Lsm)mentioning

confidence: 99%

Determination of the thickness and structure of the skin barrier by in vivo laser scanning microscopy

et al. 2007

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“…Biopsies were taken from the skin samples after application and penetration of the pollen allergens. Laser scanning microscopy [28][29][30][31][32] was used to analyze the distribution of the allergens on the histological sections. The experiments had to be carried out in vitro because of the high number of biopsies to be taken from the tissue.…”

Section: Introductionmentioning

confidence: 99%

Prevention of Follicular Penetration: Barrier-Enhancing Formulations against the Penetration of Pollen Allergens into Hair Follicles

Meinke

Patzelt

Richter

et al. 2010

Skin Pharmacol Physiol

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The hair follicles could be a reservoir for topically applied substances. They are not only surrounded by a close network of blood capillaries, which makes them interesting for drug delivery, but they are also the host of dendritic cells, which are important for immunomodulation. Previously, pollen allergens were shown to penetrate into the hair follicles. The application of barrier-enhancing formulations might represent an effective strategy to prevent pollen protein penetration into the hair follicle. In the present study, porcine skin areas were pretreated with 4 barrier-enhancing emulsions. One skin area served as control and remained without pretreatment. Afterwards, fluorescein-isothiocyanate-labeled grass pollen proteins were applied to the porcine skin samples, and their penetration was investigated via fluorescent laser scanning microscopy. It was shown that the barrier-enhancing formulations were able to significantly reduce the penetration of exogenous proteins into the hair follicles, the extent of such reduction depending on the formulation.

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“…In contrast, fluorescence and Raman spectroscopic laser scanning measurements are mostly used in cosmetics, skin physiology and pharmacology for the analysis of the distribution and the penetration process of topically applied substances [12][13][14][15][16][17][18][19][20]. Only limited reports exist in the field of clinical dermatology.…”

Section: Introductionmentioning

confidence: 99%

Application of laser spectroscopic methods for in vivo diagnostics in dermatology

Meyer¹,

Lademann²

2007

Laser Phys. Lett.

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Abstract:The importance of dermatologic non-invasive imaging techniques has increased over the last decades. Technological advancements have led to the development of various scanning tools, enabling in vivo examination of living human skin. All provide a preservation of the tissue's physical structure whilst being studied in its native state. Different modalities are currently being used to investigate the skin tissue. Although many of these scanning instruments are still undergoing research, promising imaging techniques, such as high-resolution ultrasonography, optical coherence tomography, magnetic resonance imaging and spectroscopic methods, may yet find a role in dermatologic diagnosis and disease monitoring. In this article, the authors demonstrate their own results and review the influence of laser spectroscopic methods as non-invasive diagnostic tools in dermatology. µmFluorescence image obtained 5 min after application of a dye

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Analysis of the penetration profiles of topically applied substances by laser scanning microscopy

Cited by 15 publications

References 14 publications

Determination of the thickness and structure of the skin barrier by in vivo laser scanning microscopy

Determination of the thickness and structure of the skin barrier by in vivo laser scanning microscopy

Prevention of Follicular Penetration: Barrier-Enhancing Formulations against the Penetration of Pollen Allergens into Hair Follicles

Application of laser spectroscopic methods for in vivo diagnostics in dermatology

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