Analysis of the prognostic role and biological characteristics of circulating tumor cell-associated white blood cell clusters in non-small cell lung cancer

Li, Zheng; Fan, Liwen; Wu, Yungang; Niu, Yuxu; Zhang, Xuelin; Wang, Bin; Yao, Yuanshan; Chen, Chunji; Qi, Ning; Wang, Daisy Dandan; Lin, Peter Ping; Tang, Dongfang; Gao, Wen

doi:10.21037/jtd-22-423

Cited by 11 publications

(22 citation statements)

References 37 publications

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“…Various CTC/ CEC subtypes may have di erent potential function to guide the diagnosis, prognosis prediction, and treatment decision of bladder cancer [92]. Li et al found that the presence of circulating tumor-cell-associated white blood cell (CTC-WBC) clusters is an independent prognostic factor for advanced non-small-cell lung cancer [93].…”

Section: Negative Sorting Methodsmentioning

confidence: 99%

Advances in the Biology, Detection Techniques, and Clinical Applications of Circulating Tumor Cells

Zhao

Cui

2022

Journal of Oncology

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Circulating tumor cells (CTCs) play a crucial role in tumor recurrence and metastasis, and their early detection has shown remarkable benefits in clinical theranostics. However, CTCs are extremely rare, thus detecting them in the blood is very challenging. New CTC detection techniques are continuously being developed, enabling deeper analysis of CTC biology and potential clinical application. This article reviews current CTC detection techniques and their clinical application. CTCs have provided, and will continue to provide, important insights into the process of metastasis, which could lead to development of new therapies for different cancers.

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Section: Negative Sorting Methodsmentioning

confidence: 99%

Advances in the Biology, Detection Techniques, and Clinical Applications of Circulating Tumor Cells

Zhao

Cui

2022

Journal of Oncology

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“…Interestingly, interaction with white blood cells also correlates with mixed Epithelial-mesenchymal phenotype and cancer cells polyploidy ( 21 , 68 , 69 ) that play a key role in cancer resistance to treatment and metastasis ( 37 , 70 – 72 ).…”

Section: Polyploidy and Epithelial-to-mesenchymal Transition In Ctc C...mentioning

confidence: 99%

“…The presence of CTC-WBC clusters was associated with worse prognosis in lung ( 21 , 22 ), breast cancers ( 19 ), and hepatocellular carcinoma ( 73 , 74 ). Remarkably, in lung cancer, CTCs in complex with WBCs were exclusively polyploid ( 21 ).…”

Section: Polyploidy and Epithelial-to-mesenchymal Transition In Ctc C...mentioning

confidence: 99%

“…A recent paper employed the iFISH method combining FISH DNA staining and immunofluorescence ( 21 , 22 ) to create Atlas of Circulating Rare Cells ( 69 ). High throughput imaging analysis of circulating rare cells (CRCs) purified by WBC subtraction categorized cells into 71 subtypes based on the CD45 leukocyte staining, cell size, chromosome 8 ploidy and the presence of a few tumor cell markers including PD-L1 (EPCAM/CK18/PD-L1/AFP/HER2/CA19-9), endothelial CD31, mesenchymal Vimentin and stem cell CD133 markers ( 69 ).…”

Section: Polyploidy and Epithelial-to-mesenchymal Transition In Ctc C...mentioning

confidence: 99%

“…Their expression promotes formation of CTC clusters and metastasis by inducing adhesion ( 4 , 30 , 33 ), proliferation ( 16 ), by metabolic adaptation to oxidative stress ( 17 , 34 ), and through the epithelial-mesenchymal transition ( 31 , 35 ). Quite intriguing, in lung cancer most CTCs interacting with WBCs were polyploid ( 21 ) thus, implying repression of the mitotic checkpoint, induction of cell survival and migration ( 36 – 38 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecules promoting circulating clusters of cancer cells suggest novel therapeutic targets for treatment of metastatic cancers

Rozenberg

Buzdin²,

Mohammad³

et al. 2023

Front. Immunol.

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Treatment of metastatic disease remains among the most challenging tasks in oncology. One of the early events that predicts a poor prognosis and precedes the development of metastasis is the occurrence of clusters of cancer cells in the blood flow. Moreover, the presence of heterogeneous clusters of cancerous and noncancerous cells in the circulation is even more dangerous. Review of pathological mechanisms and biological molecules directly involved in the formation and pathogenesis of the heterotypic circulating tumor cell (CTC) clusters revealed their common properties, which include increased adhesiveness, combined epithelial-mesenchymal phenotype, CTC-white blood cell interaction, and polyploidy. Several molecules involved in the heterotypic CTC interactions and their metastatic properties, including IL6R, CXCR4 and EPCAM, are targets of approved or experimental anticancer drugs. Accordingly, analysis of patient survival data from the published literature and public datasets revealed that the expression of several molecules affecting the formation of CTC clusters predicts patient survival in multiple cancer types. Thus, targeting of molecules involved in CTC heterotypic interactions might be a valuable strategy for the treatment of metastatic cancers.

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Research progress on the multi-omics and survival status of circulating tumor cells

Xie,

Liu,

Zhang

et al. 2024

Clin Exp Med

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In the dynamic process of metastasis, circulating tumor cells (CTCs) emanate from the primary solid tumor and subsequently acquire the capacity to disengage from the basement membrane, facilitating their infiltration into the vascular system via the interstitial tissue. Given the pivotal role of CTCs in the intricate hematogenous metastasis, they have emerged as an essential resource for a deeper comprehension of cancer metastasis while also serving as a cornerstone for the development of new indicators for early cancer screening and new therapeutic targets. In the epoch of precision medicine, as CTC enrichment and separation technologies continually advance and reach full fruition, the domain of CTC research has transcended the mere straightforward detection and quantification. The rapid advancement of CTC analysis platforms has presented a compelling opportunity for in-depth exploration of CTCs within the bloodstream. Here, we provide an overview of the current status and research significance of multi-omics studies on CTCs, including genomics, transcriptomics, proteomics, and metabolomics. These studies have contributed to uncovering the unique heterogeneity of CTCs and identifying potential metastatic targets as well as specific recognition sites. We also review the impact of various states of CTCs in the bloodstream on their metastatic potential, such as clustered CTCs, interactions with other blood components, and the phenotypic states of CTCs after undergoing epithelial-mesenchymal transition (EMT). Within this context, we also discuss the therapeutic implications and potential of CTCs.

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Analysis of the prognostic role and biological characteristics of circulating tumor cell-associated white blood cell clusters in non-small cell lung cancer

Cited by 11 publications

References 37 publications

Advances in the Biology, Detection Techniques, and Clinical Applications of Circulating Tumor Cells

Advances in the Biology, Detection Techniques, and Clinical Applications of Circulating Tumor Cells

Molecules promoting circulating clusters of cancer cells suggest novel therapeutic targets for treatment of metastatic cancers

Research progress on the multi-omics and survival status of circulating tumor cells

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