Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet

Grilli, Diego; Fliegerová, K.; Kopečný, J.; Lama, S. Paez; Egea, Vanina; Sohaefer, Noelia; Pereyra, Celia; Ruiz, María Soledad; Sosa, Miguel A. Gama; Arenas, Graciela Nora; Mrázek, Jakub

doi:10.1016/j.anaerobe.2016.07.002

Cited by 49 publications

(30 citation statements)

References 54 publications

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“…The establishment of the gut bacterial community has been shown to be a progressive process with an increasing diversity and changing composition, which is necessary for development and health of hosts (Jami et al, 2013). Another important aspect of microbial communities is the Firmicutes to Bacteroidetes ratio, as it has been shown to be of relevance in signaling relationship between gut microbial status and aging (Ley et al, 2006; Grilli et al, 2016). Different bacteria must evolve the appropriate strategies and physiological traits to successfully occupy a niche that remains stable during the development of a host (Jami et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing

Liu

Shafer

et al. 2017

Front. Microbiol.

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The gut ecosystem is characterized by dynamic and reciprocal interactions between the host and bacteria. Although characterizing microbiota for herbivores has become recognized as important tool for gauging species health, no study to date has investigated the bacterial communities and evaluated the age-related bacterial dynamics of musk deer. Moreover, gastrointestinal diseases have been hypothesized to be a limiting factor of population growth in captive musk deer. Here, high-throughput sequencing of the bacterial 16S rRNA gene was used to profile the fecal bacterial communities in juvenile and adult alpine and forest musk deer. The two musk deer species harbored similar bacterial communities at the phylum level, whereas the key genera for the two species were distinct. The bacterial communities were dominated by Firmicutes and Bacteroidetes, with the bacterial diversity being higher in forest musk deer. The Firmicutes to Bacteroidetes ratio also increased from juvenile to adult, while the bacterial diversity, within-group and between-group similarity, all increased with age. This work serves as the first sequence-based analysis of variation in bacterial communities within and between musk deer species, and demonstrates how the gut microbial community dynamics vary among closely related species and shift with age. As gastrointestinal diseases have been observed in captive populations, this study provides valuable data that might benefit captive management and future reintroduction programs.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing

Liu

Shafer

et al. 2017

Front. Microbiol.

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“…The change from high forage to high concentrate diet is accompanied by a significant alteration in ruminal microbial populations (Silva and Leão 1979). This is due to changes in the biochemical structure of feed ingredients, preference of microbes for a particular substrate and overall changes in the rumen pH; especially in a diet containing more than 60% concentrate (Gaafar et al 2009;Grilli et al 2016).…”

Section: Diet and Rumen Microbial Shiftmentioning

confidence: 99%

Role of diverse fermentative factors towards microbial community shift in ruminants

et al. 2019

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Summary Besides the liver, rumen is one of the most important components of metabolism in ruminants. However, the microbes residing in the rumen are influenced by several complex factors such as diet, which result in fluctuations in the rumen pH. Rumen pH affects feed intake and feed digestibility, subsequently causing microbial shift in the individual members of microbial community residing in the foregut and hindgut. This in turn causes an increase in lipopolysaccharide concentration, among other factors, in the gut fluid and animal blood. Irrespective of diet fed to animals, Firmicutes would probably be the most dominant in high grain diet while Bacteroidetes are dominant in hay diet, and both have a relative abundance of about 80% or more at times. The shift in microbial population is not limited to adult ruminants alone but also occur in calves. Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria were the most abundant in both hay and concentrate diet of newly weaned calves. Prolonged, depressed pH, causes subacute ruminal acidosis. This leads to compromise in the integrity of both foregut and hindgut of ruminants, eventually causing structural changes in the gut physiology. Furthermore, diet containing C‐12:0 and C‐14:0, which are medium‐chain fatty acids, were toxic to rumen protozoa. Phytochemical content in some plant residues when fed to animals also causes shift in microbial population. Therefore, foregut and hindgut pH stability is important for ruminant health and for optimal productivity.

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“…For example, Wang et al investigated the temporal dynamics of the rumen microbiome in goats, and observed that the relative abundance of Firmicutes did not vary with age [9]. Considering the dietary composition or source, the rumen bacterial diversity increased in goats that were fed a forage diet compared to a mixed forage-concentrate diet [16]. Similarly, the relative abundance of Bacteroidetes decreased significantly in the rumen of cashmere goats when the dietary forage to concentrate ratio was decreased [17].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Rumen Microbiota and Volatile Fatty Acid Profiles of Weaned Goat Kids under Shrub-Grassland Grazing and Indoor Feeding

Guo

Liu

et al. 2020

Animals

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In this study, we conducted comparative analyses to characterize the rumen microbiota and volatile fatty acid (VFA) profiles of weaned Nanjiang Yellow goat kids under shrub-grassland grazing (GR), shrub-grassland grazing and supplementary feeding (SF), and indoor feeding (IF) systems. We observed significant differences (p < 0.05) in the concentrations of total VFA and the proportions of acetate and butyrate in the rumen fluid among the three groups, whereas the proportions of propionate and the acetate/propionate ratio did not differ substantially. Alpha diversity of the rumen bacterial and archaeal populations in the GR and SF kids was significantly higher (p < 0.05) than that in the IF goat kids, and significant differences (p < 0.05) in similarity were observed in the comparisons of GR vs. IF and SF vs. IF. The most predominant bacterial phyla were Bacteroidetes and Firmicutes across the three groups, and the archaeal community was mainly composed of Euryarchaeota. At the genus and species levels, the cellulose-degrading bacteria, including Lachnospiraceae, Ruminococcaceae and Butyrivibrio fibrisolvens, were abundant in the GR and SF groups. Furthermore, 27 bacterial and 11 unique archaeal taxa, such as Lachnospiraceae, Butyrivibrio fibrisolvens, and Methanobrevibacter ruminantium, were identified as biomarkers, and showed significantly different (p < 0.05) abundances among the three groups. Significant Spearman correlations (p < 0.05), between the abundances of several microbial biomarkers and the concentrations of VFAs, were further observed. In summary, our results demonstrated that the adaptation to grazing required more rumen bacterial populations due to complex forage types in shrub-grassland, although the rumen fermentation pattern did not change substantially among the three feeding systems. Some microbial taxa could be used as biomarkers for different feeding systems, particularly cellulose-degrading bacteria associated with grazing.

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Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet

Cited by 49 publications

References 54 publications

Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing

Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing

Role of diverse fermentative factors towards microbial community shift in ruminants

Characterization of the Rumen Microbiota and Volatile Fatty Acid Profiles of Weaned Goat Kids under Shrub-Grassland Grazing and Indoor Feeding

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