Analysis of the sealing ability of different obturation techniques in oval-shaped canals: a study using a bacterial leakage model

Abstract

The aim of this study was to evaluate the sealing ability of 3 obturation techniques in oval canals using a bacterial leakage model. Seventy mandibular incisors with oval canals were selected after buccolingual and mesiodistal radiographs, and divided into three groups. After instrumentation, the canals were obturated respectively by lateral condensation (G1), warm vertical condensation (G2) and Thermafil (G3). Five root canals were not obturated and served as positive controls; five teeth with intact crowns served as negative controls. All teeth were mounted on a 2 chamber apparatus and then exposed to human saliva. The number of days required for the entire contamination of the root canals was recorded, observing the turbidity of the BHI broth, for a period of 100 days. 84.21% of the samples from the lateral condensation group (G1); 84.21% of the samples from the warm vertical condensation group (G2); and 89.47% of the samples from the Thermafil System group (G3) showed no contamination at the end of the study. No statistical significant difference was found among the three techniques. The quality of the apical seal in the three techniques tested was similar.

Comparison of three obturation techniques with regard to bacterial leakage

“…Various methods have been employed in the evaluation of the obturation of root canals: dye leakage [12][13] , fluid filtration [14][15][16] , and bacterial leakage [17][18][19][20][21][22][23][24] . The option for bacterial leakage is justified since it is an experimental model that is simple and easy to replicate.…”

“…Most studies have used single-rooted teeth [17][18][19][20][21][22][23][24] . The use of molars was proposed due to their high incidence in endodontic treatment, and the choice of the distobuccal root of the maxillary molar was based on a standardization criterion 9 .…”

“…The use of molars was proposed due to their high incidence in endodontic treatment, and the choice of the distobuccal root of the maxillary molar was based on a standardization criterion 9 . Various bacteria have been used in microleakage studies [17][18]21,25 . However, there is a focus on E. faecalis because of its resistance [19][20]22,24 , survival in nutrient-poor areas over long periods 26 , and association with persistent chronic periapical lesions 27 .…”

Limited Ability of Three Commonly Used Thermoplasticized Gutta-Percha Techniques in Filling Oval-shaped Canals

“…Periapical radiographs were taken in the buccolingual and mesiodistal directions in each tooth selected with a long:short diameter ratio of Ն2.5 at 5 mm from the apex (7)(8)(9). Therefore, only 87 incisors were classified as single oval-shaped canals; 7 were discarded, leaving a total sample of 80 teeth.…”

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

“…1 When new sealing materials or techniques emerge, studies should be performed to warrant their clinical application and to accurately assess their characteristics and properties, particularly their sealing ability. Several methods aimed at detecting leakage have already been described in the literature, such as the conductometric technique, 2 fluid filtration, 3,4 radioisotopes, 5 bacterial penetration, 6 the electrochemical test, 7 and the use of dyes. 8,9 However, all these methods have yielded controversial results, and have therefore been widely criticized.…”