Analysis of UV-induced mutation spectra in Escherichia coli by DNA polymerase η from Arabidopsis thaliana

Santiago, María Jesús; Alejandre-Durán, Encarna; Ruiz-Rubio, Manuel

doi:10.1016/j.mrfmmm.2006.05.036

Cited by 31 publications

(50 citation statements)

References 65 publications

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“…Some of these have been characterized. For instance, it was shown that a cDNA encoding DNA pol h of Arabidopsis is able to complement a UV-sensitive yeast strain deficient in this polymerase (Santiago et al, 2006). Increased UV sensitivity was also reported for a Rev1 mutant (Takahashi et al, 2005).…”

Section: Discussionmentioning

confidence: 95%

Oxidative DNA Damage Bypass in Arabidopsis thaliana Requires DNA Polymerase λ and Proliferating Cell Nuclear Antigen 2

et al. 2011

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The oxidized base 7,8-oxoguanine (8-oxo-G) is the most common DNA lesion generated by reactive oxygen species. This lesion is highly mutagenic due to the frequent misincorporation of A opposite 8-oxo-G during DNA replication. In mammalian cells, the DNA polymerase (pol) family X enzyme DNA pol l catalyzes the correct incorporation of C opposite 8-oxo-G, together with the auxiliary factor proliferating cell nuclear antigen (PCNA). Here, we show that Arabidopsis thaliana DNA pol l, the only member of the X family in plants, is as efficient in performing error-free translesion synthesis past 8-oxo-G as its mammalian homolog. Arabidopsis, in contrast with animal cells, possesses two genes for PCNA. Using in vitro and in vivo approaches, we observed that PCNA2, but not PCNA1, physically interacts with DNA pol l, enhancing its fidelity and efficiency in translesion synthesis. The levels of DNA pol l in transgenic plantlets characterized by overexpression or silencing of Arabidopsis POLL correlate with the ability of cell extracts to perform error-free translesion synthesis. The important role of DNA pol l is corroborated by the observation that the promoter of POLL is activated by UV and that both overexpressing and silenced plants show altered growth phenotypes.

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Section: Discussionmentioning

confidence: 95%

Oxidative DNA Damage Bypass in Arabidopsis thaliana Requires DNA Polymerase λ and Proliferating Cell Nuclear Antigen 2

et al. 2011

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“…nucleotide excision repair and HR) are conserved in higher plants (Britt, 1999;Kimura and Sakaguchi, 2006). After the recent identification of the translesion polymerases (Pol h, Pol z, and Pol k) in Arabidopsis (Arabidopsis thaliana), the existence of at least two branches of PRR seems to be evident for plants, too (Sakamoto et al, 2003;GarciaOrtiz et al, 2004;Takahashi et al, 2005;Santiago et al, 2006). However, until recently no indication had been provided for the existence of a Rad5-dependent branch of PRR in plants.…”

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confidence: 99%

A Homolog ofScRAD5Is Involved in DNA Repair and Homologous Recombination in Arabidopsis

et al. 2008

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Rad5 is the key component in the Rad5-dependent error-free branch of postreplication repair in yeast (Saccharomyces cerevisiae). Rad5 is a member of the Snf2 ATPase/helicase family, possessing as a characteristic feature, a RING-finger domain embedded in the Snf2-helicase domain and a HIRAN domain. Yeast mutants are sensitive to DNA-damaging agents and reveal differences in homologous recombination. By sequence comparisons we were able to identify two homologs (AtRAD5a and AtRAD5b) in the Arabidopsis thaliana genome, sharing about 30% identity and 45% similarity to yeast Rad5. AtRad5a and AtRad5b have the same kind of domain organization with a higher degree of similarity to each other than to ScRad5. Surprisingly, both genes differ in function: whereas two independent mutants of Atrad5a are hypersensitive to the cross-linking agents mitomycin C and cis-platin and to a lesser extent to the methylating agent, methyl methane sulfonate, the Atrad5b mutants did not exhibit any sensitivity to all DNA-damaging agents tested. An Atrad5a/Atrad5b double mutant resembles the sensitivity phenotype of the Atrad5a single mutants. Moreover, in contrast to Atrad5b, the two Atrad5a mutants are deficient in homologous recombination after treatment with the double-strand break-inducing agent bleomycin. Our results suggest that the RAD5-dependent error-free branch of postreplication repair is conserved between yeast and plants, and that AtRad5a might be functionally homologous to ScRad5.

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“…In mammalian cells, it is generally accepted that Rad18 is required for PCNA monoubiquitination, which in turn recruits Y-family polymerases (Bienko et al, 2005;Kannouche et al, 2004). A number of reports have addressed the in vitro and in vivo activities of plant TLS polymerases (Anderson et al, 2008;Curtis and Hays, 2007;Garcia-Ortiz et al, 2004;Sakamoto et al, 2003;Santiago et al, 2006;Takahashi et al, 2005); however, the requirement of PCNA ubiquitination for their in vivo activity has not been reported, although there is an indirect observation that AtPCNA2 can stimulate AtPol activity in yeast cells (Anderson et al, 2008).…”

Section: Two Alternative Ddt Pathways Represented By Atrad5a and Atrev3mentioning

confidence: 99%

RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis

Wang¹,

Wen²,

Shi³

et al. 2011

DNA Repair

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Analysis of UV-induced mutation spectra in Escherichia coli by DNA polymerase η from Arabidopsis thaliana

Cited by 31 publications

References 65 publications

Oxidative DNA Damage Bypass in Arabidopsis thaliana Requires DNA Polymerase λ and Proliferating Cell Nuclear Antigen 2

Oxidative DNA Damage Bypass in Arabidopsis thaliana Requires DNA Polymerase λ and Proliferating Cell Nuclear Antigen 2

A Homolog ofScRAD5Is Involved in DNA Repair and Homologous Recombination in Arabidopsis

RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis

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