Analysis of wax esters in edible oils by automated on-line coupling liquid chromatography–gas chromatography using the through oven transfer adsorption desorption (TOTAD) interface

Aragón, Álvaro; Cortés, José M.; Toledano, Rosa M.; Villén, Jesús; Vázquez, Ana María

doi:10.1016/j.chroma.2010.12.042

Cited by 20 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, Carnauba wax obtained from the epicuticular wax of the palm Copernicia prunifera has economical relevance as a commodity for polishes, food coatings and cosmetic products [28]. Wax ester profiling is also taken as a mean to evaluate the quality of edible oils, especially olive oil, that contains residual amounts of wax esters residing from the seed or fruit coat [32,33]. …”

Section: Introductionmentioning

confidence: 99%

Wax ester profiling of seed oil by nano-electrospray ionization tandem mass spectrometry

et al. 2013

View full text Add to dashboard Cite

BackgroundWax esters are highly hydrophobic neutral lipids that are major constituents of the cutin and suberin layer. Moreover they have favorable properties as a commodity for industrial applications. Through transgenic expression of wax ester biosynthetic genes in oilseed crops, it is possible to achieve high level accumulation of defined wax ester compositions within the seed oil to provide a sustainable source for such high value lipids. The fatty alcohol moiety of the wax esters is formed from plant-endogenous acyl-CoAs by the action of fatty acyl reductases (FAR). In a second step the fatty alcohol is condensed with acyl-CoA by a wax synthase (WS) to form a wax ester. In order to evaluate the specificity of wax ester biosynthesis, analytical methods are needed that provide detailed wax ester profiles from complex lipid extracts.ResultsWe present a direct infusion ESI-tandem MS method that allows the semi-quantitative determination of wax ester compositions from complex lipid mixtures covering 784 even chain molecular species. The definition of calibration prototype groups that combine wax esters according to their fragmentation behavior enables fast quantitative analysis by applying multiple reaction monitoring. This provides a tool to analyze wax layer composition or determine whether seeds accumulate a desired wax ester profile. Besides the profiling method, we provide general information on wax ester analysis by the systematic definition of wax ester prototypes according to their collision-induced dissociation spectra. We applied the developed method for wax ester profiling of the well characterized jojoba seed oil and compared the profile with wax ester-accumulating Arabidopsis thaliana expressing the wax ester biosynthetic genes MaFAR and ScWS.ConclusionsWe developed a fast profiling method for wax ester analysis on the molecular species level. This method is suitable to screen large numbers of transgenic plants as well as other wax ester samples like cuticular lipid extracts to gain an overview on the molecular species composition. We confirm previous results from APCI-MS and GC-MS analysis, which showed that fragmentation patterns are highly dependent on the double bond distribution between the fatty alcohol and the fatty acid part of the wax ester.

show abstract

Section: Introductionmentioning

confidence: 99%

Wax ester profiling of seed oil by nano-electrospray ionization tandem mass spectrometry

et al. 2013

View full text Add to dashboard Cite

show abstract

“…However, Michael-Jubeli et al [111] reported a method to analyze waxes and other lipids in skin surface lipid samples by GC-MS without prior isolation of the waxes. Alternatively, isolation of the wax fraction from the other lipids can be done on-line using liquid chromatography coupled with GC as reported by Aragon et al [110].…”

Section: Analysis Of Waxesmentioning

confidence: 99%

“…Analysis of waxes can be done by GC and most recent references report the use of MS as detector [109,110]. The standard procedures for GC analysis of waxes involve as a first step the purification of the waxes from TAG and other lipids by silica gel chromatography, thin-layer chromatography, or solid-phase extraction [1].…”

Section: Analysis Of Waxesmentioning

confidence: 99%

Analysis of Lipids by Gas Chromatography

Cruz‐Hernandez¹,

Destaillats²

2012

Gas Chromatography

View full text Add to dashboard Cite

“…This interface, which is totally automatic, was a major breakthrough in the field of multidimensional LC-GC, because it allows the use of normal as well as reversed phase in the LC step even using a mass spectrometer (MS) detector [14]. Several analytical methods have been developed in reverse [15,16] and normal phase [17,18]. The TOTAD interface has also been used in the development of analytical methods for LVI, including the direct injection of water into the GC [14,19].…”

Section: Introductionmentioning

confidence: 99%

Analysis of polycyclic aromatic hydrocarbons in aqueous samples by large volume injection gas chromatography–mass spectrometry using the through oven transfer adsorption desorption interface

Aragón

Toledano

Vázquez

et al. 2015

Talanta

Self Cite

View full text Add to dashboard Cite

Analysis of wax esters in edible oils by automated on-line coupling liquid chromatography–gas chromatography using the through oven transfer adsorption desorption (TOTAD) interface

Cited by 20 publications

References 19 publications

Wax ester profiling of seed oil by nano-electrospray ionization tandem mass spectrometry

Wax ester profiling of seed oil by nano-electrospray ionization tandem mass spectrometry

Analysis of Lipids by Gas Chromatography

Analysis of polycyclic aromatic hydrocarbons in aqueous samples by large volume injection gas chromatography–mass spectrometry using the through oven transfer adsorption desorption interface

Contact Info

Product

Resources

About