Analysis of Xenopus laevis RECK and Its Relationship to Other Vertebrate RECK Sequences

Willson, Jessica A.; Nieuwesteeg, Michelle A.; Cepeda, Mario; Damjanovski, Sashko

doi:10.9734/jsrr/2015/17044

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…Another 24 h following transfection, cell lysates were collected. The generation of the full-length RECK cDNA construct is described in [13], with the HA tag being inserted just following the N-terminal signal sequence such that it would not be removed during secretion, nor would it interfere with GPI anchor formation at the C-terminal end.…”

Section: Methodsmentioning

confidence: 99%

“…This suggests a mechanism that may involve more molecules than just MT1-MMP in the activation of ERK and pro-MMP-2, with RECK possibly playing an important role. We have previously cloned and characterized the RECK gene and studied its expression during Xenopus laevis development, showing that RECK is present at stages where ECM remodeling events are associated with neural function [13]. Our recent in vivo examination of RECK, MT1-MMP, and TIMP-2 show that these proteins colocalize in the dorsal axis of 48-h Xenopus tailbud stage embryos, particularly in the neural tube [14].…”

Section: Introductionmentioning

confidence: 99%

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Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levels

Willson

Bork

Muir

et al. 2019

J of Biol Res-Thessaloniki

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BackgroundMT1-MMP is a cell-surface enzyme whose regulation of pro-MMP-2 and ERK activation position it as a key facilitator of ECM remodelling and cell migration. These processes are modulated by endogenous MMP inhibitors, such as RECK, a GPI-anchored protein which has been shown to inhibit both MT1-MMP and MMP-2 activity. Our previous studies have revealed a link between MT1-MMP levels, and pro-MMP-2 and ERK activation in mammalian cells, as well as MT1-MMP and RECK co-localization in Xenopus embryos. We here investigated how modulation of RECK would impact MT1-MMP and MMP-2 levels, as well as ERK signalling in Xenopus A6 cells.ResultsWe used a Morpholino approach to knockdown RECK, plasmid transfection to overexpress RECK, and PI-PLC treatment to shed RECK from the cell surface of Xenopus A6 cells. RECK reduction did not alter pERK or MT1-MMP levels, nor MMP-2 activity as measured by zymography; thus RECK-knockdown cells maintained the ability to remodel the ECM. RECK overexpression and PI-PLC treatment both increased ECM remodelling potential through increased MT1-MMP protein and relative MMP-2 activation levels.ConclusionsRECK changes that reduce the ability of the cell to remodel the ECM (overexpression and cell surface shedding) are compensated for by increases in MT1-MMP, and MMP-2 levels as seen by zymography.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levels

Willson

Bork

Muir

et al. 2019

J of Biol Res-Thessaloniki

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Spatial analysis of RECK, MT1-MMP, and TIMP-2 proteins during early Xenopus laevis development

Willson

Damjanovski

2019

Gene Expression Patterns

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Dynamic expression of MMP28 during cranial morphogenesis

Gouignard

Théveneau

Saint-Jeannet

2020

Phil. Trans. R. Soc. B

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Matrix metalloproteinases (MMPs) are a large family of proteases comprising 24 members in vertebrates. They are well known for their extracellular matrix remodelling activity. MMP28 is the latest member of the family to be discovered. It is a secreted MMP involved in wound healing, immune system maturation, cell survival and migration. MMP28 is also expressed during embryogenesis in human and mouse. Here, we describe the detailed expression profile of MMP28 in Xenopus laevis embryos. We show that MMP28 is expressed maternally and accumulates at neurula and tail bud stages specifically in the cranial placode territories adjacent to migrating neural crest cells. As a secreted MMP, MMP28 may be required in neural crest–placode interactions. This article is part of a discussion meeting issue ‘Contemporary morphogenesis’.

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Analysis of Xenopus laevis RECK and Its Relationship to Other Vertebrate RECK Sequences

Cited by 4 publications

References 16 publications

Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levels

Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levels

Spatial analysis of RECK, MT1-MMP, and TIMP-2 proteins during early Xenopus laevis development

Dynamic expression of MMP28 during cranial morphogenesis

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