Analysis of α-Tubulin Gene Expression During Cold Acclimation of Winter and Spring Soft Wheat

Demkovych, A. E.; Pirko, Ya. V.; Blume, Ya. B.

doi:10.3103/s0095452719010067

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…However, many studies have shown that the expressions of almost all TUA and TUB genes are controlled both spatially and temporally during plant growth, development, and the process of responding to specific signals. For example, α-tubulin genes play an important role in wheat's cold acclimation and low-temperature tolerance [33,34]. The different expression patterns of tubulins showed that tubulin subtypes might play specific roles during plant development [22].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of TUA and TUB Genes in Wheat (Triticum aestivum L.) during Its Development

Yang

Song

Shan

et al. 2022

Plants

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Microtubules play a fundamental role in plant development, morphogenesis, and cytokinesis; they are assembled from heterodimers containing an α-tubulin (TUA) and a β-tubulin (TUB) protein. However, little research has been conducted on the TUA and TUB gene families in hexaploid wheat (Triticum aestivum L.). In this study, we identified 15 TaTUA and 28 TaTUB genes in wheat. Phylogenetic analysis showed that 15 TaTUA genes were divided into two major subfamilies, and 28 TaTUB genes were divided into five major subfamilies. Mostly, there were similar motif compositions and exon-intron structures among the same subfamilies. Segmental duplication of genes (WGD/segmental) is the main process of TaTUA and TaTUB gene family expansion in wheat. It was found that TaTUA and TaTUB genes presented specific temporal and spatial characteristics based on the expression profiles of 17 tissues during wheat development using publicly available RNA-seq data. It was worth noting, via qRT-PCR, that two TaTUA and five TaTUB genes were highly expressed in fertile anthers compared to male sterility. These were quite different between physiological male sterile lines and S-type cytoplasmic male sterile lines at different stages of pollen development. This study offers fundamental information on the TUA and TUB gene families during wheat development and provides new insights for exploring the molecular mechanism of wheat male sterility.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of TUA and TUB Genes in Wheat (Triticum aestivum L.) during Its Development

Yang

Song

Shan

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…Burch and Marchant (1983) reported Pyramimonas gelidicola is more cold adaptable at −14 °C due to its stronger stabilization of cytoskeletal microtubules. Recent studies have revealed that the increase in expression of tubulin in early stages of cold acclimation is related to cold resistance (Buy et al., 2019). It has been found that the expression of the SoTUA gene was higher in cold−resistant plants than cold−susceptible plants under cold stress (Zhang et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

et al. 2021

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Alpha (α)-tubulin (TUA) is a primary unit of cytoskeleton microtubules. In recent years, more frequent frosts have caused considerable yield loss in sugarcane (Saccharum spp. hybrid) production. Temperature is one of the most influential abiotic factors affecting the microtubule polymerization state in plants. In this research, we developed overexpressing SoTUA transgenic sugarcane lines from the cold-susceptible cultivar ROC22. The transgene was confirmed by polymerase chain reaction (PCR) and quantificational real-time polymerase chain reaction (qRT-PCR) analysis, while the relative expression of SoTUA protein was verified by Western blot. Compared with the nontransformed control plants (wild type [WT]), the transgenic lines showed higher relative protein expression than the WT at 25 ˚C, which indicates the overexpression of SoTUA protein. The contents of soluble protein and sugar and the activity of peroxidase (POD) were higher while the content of maleicdialdehyde was lower in the transgenic plants than the WT plants under the chilling treatment. Meanwhile, the expression levels of cold-defense related genes (P5CS, WRKY, Cu/Zn-SOD, and POD1) were higher in the transgenic lines than in the WT. These results suggest that the SoTUA protein plays an important role in protecting plants during chilling stress. This positive effect is not affected by transgenic plant generations.

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Overexpression of α-Tubulin Gene of Sugarcane (Saccharum spp. hybrids), SoTUA, Enhances Tobacco Tolerance to Cold Stress

et al. 2022

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Analysis of α-Tubulin Gene Expression During Cold Acclimation of Winter and Spring Soft Wheat

Cited by 5 publications

References 26 publications

Genome-Wide Identification and Expression Analysis of TUA and TUB Genes in Wheat (Triticum aestivum L.) during Its Development

Genome-Wide Identification and Expression Analysis of TUA and TUB Genes in Wheat (Triticum aestivum L.) during Its Development

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

Overexpression of α-Tubulin Gene of Sugarcane (Saccharum spp. hybrids), SoTUA, Enhances Tobacco Tolerance to Cold Stress

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