Analytical approaches to poly-γ-glutamate: Quantification, molecular size determination, and stereochemistry investigation

Ashiuchi, Makoto

doi:10.1016/j.jchromb.2011.03.029

Cited by 29 publications

(16 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The molecular weight of the γ‐PGA produced by B. licheniformis CGMCC 2876 involved in 2.0 × 10 6 Da, 6.0 × 10 5 Da, and 2.0 × 10 5 Da, and the percentage of 2.0 × 10 6 Da decreased when the concentration of trisodium citrate increased (Figure c). Generally, the molecular weight of γ‐PGA produced by different strains varies from10 4 Da to 10 6 Da and is affected by the nutrients in the medium . The molecular weight of γ‐PGA produced from B. subtilis (chungkookjang) and B. licheniformis ATCC 9945A were influenced by the concentration of NaCl and Mn 2+ , respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, the molecular weight of g-PGA produced by different strains varies from10 4 Da to 10 6 Da and is affected by the nutrients in the medium. 32 The molecular weight of g-PGA produced from B. subtilis (chungkookjang) 30 and B. licheniformis ATCC 9945A 31 were influenced by the concentration of NaCl and Mn 21 , respectively.…”

Section: The Effects Of Molecular Weight On Flocculating Activity Of mentioning

confidence: 99%

See 1 more Smart Citation

Poly-γ-glutamic acid produced fromBacillus licheniformisCGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Yan

Yao

Chen

et al. 2015

Biotechnol Progress

View full text Add to dashboard Cite

As an environmentally friendly and industrially useful biopolymer, poly-γ-glutamic acid (γ-PGA) from Bacillus licheniformis CGMCC 2876 was characterized by the high-resolution mass spectrometry and (1)H NMR. A flocculating activity of 11,474.47 U mL(-1) obtained with γ-PGA, and the effects of carbon sources, ions, and chemical properties (D-/L-composition and molecular weight) on the production and flocculating activity of γ-PGA were discussed. Being a bioflocculant in the sugar refinery process, the color and turbidity of the sugarcane juice was IU 1,877.36 and IU 341.41 with 0.8 ppm of γ-PGA, respectively, which was as good as the most widely used chemically synthesized flocculant in the sugarcane industry--polyacrylamide with 1 ppm. The γ-PGA produced from B. licheniformis CGMCC 2876 could be a promising alternate of chemically synthesized flocculants in the sugarcane industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: The Effects Of Molecular Weight On Flocculating Activity Of mentioning

confidence: 99%

Poly-γ-glutamic acid produced fromBacillus licheniformisCGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Yan

Yao

Chen

et al. 2015

Biotechnol Progress

View full text Add to dashboard Cite

show abstract

“…Recently, poly-γ-glutamic acid (γ-PGA) has gained the attention of agronomists and soil scientists for its water soluble, anionic, biodegradable, and biocompatible properties (Sung et al 2005;Ho et al 2006;Ashiuchi 2011;Bajaj and Singhal 2011). γ-PGA is a biosynthetic anionic homo-polyamide that consists of D/L-glutamic acid units connected by amide linkages between α-amine and γ-carboxylic acid groups (Chibnall et al 1958;Shih and Van 2001) and possesses a nylon-like backbone with a carboxylic group attached to the α-carbon of every repeated unit (Portilla-Arias et al 2007;Ashiuchi 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of poly-γ-glutamic acid (γ-PGA) on soil nitrogen and carbon leaching and CO2 fluxes in a sandy clay loam soil

Zhang

Gao

et al. 2017

Can. J. Soil. Sci.

View full text Add to dashboard Cite

Poly-γ-glutamic acid (γ-PGA) has shown a significant promotional effect on plant production. However, little is known of the environmental footprint generated from the application of γ-PGA. A laboratory trial was conducted to study the effects of γ-PGA on soil nitrogen and carbon leaching loss and carbon dioxide (CO 2) emission by applying 0, 0.0125, 0.025, 0.05, 0.1, 0.2, 0.4, and 0.8 g γ-PGA kg −1 soil to soil receiving 150 kg N ha −1 in the form of urea. Results showed that the cumulative loss of ammonium and nitrate decreased by 17.81%-29.31% and 8.27%-52.42% when the application rate of γ-PGA reached 0.1 g kg −1 soil. Cumulative total dissolved nitrogen loss was diminished by 7.16%-40.10% when the γ-PGA application rate was 0.2-0.8 g kg −1 soil. Cumulative loss of dissolved organic carbon was rarely affected by the γ-PGA, whereas cumulative CO 2 flux was notably enhanced by 26.87%-180.70%. Soil total nitrogen (TN) and soil organic carbon (SOC) contents varied with the different application rates of γ-PGA; soil TN increased by 6.34%-8.04%, and SOC remained unchanged only when the γ-PGA application rate was 0.4-0.8 g kg −1 soil. In conclusion, before considering using γ-PGA in an agroecosystem, its effects on both the environment and plant production should be examined.

show abstract

“…Natto contains γ-PGA produced by Bacillus subtilis (natto) during fermentation process [2]. High-performance liquid chromatography (HPLC), sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the cetyltrimethylammonium bromide (CTAB)-dependent spectrophotometric assay are used for quantification of γ-PGA [3].…”

Section: Introductionmentioning

confidence: 99%

Effects of <i>γ</i>-Polyglutamic Acid on Blood Glucose and Caecal Short Chain Fatty Acids in Adult Male Mice

Tamura¹,

Hori²,

Inose³

et al. 2020

FNS

View full text Add to dashboard Cite

γ-Polyglutamic acid (γ-PGA) is a major component of Natto. We hypothesized that γ-PGA could reduce postprandial glucose rise and plasma glucose levels. Mice were fed a 0.1% γ-PGA-containing diet or control diet for 91 days. Maltose and starch tolerance tests were performed, and plasma lipids, glucose levels, and caecal short chain fatty acids (SCFAs) were measured. Mice were co-administered γ-PGA and starch to suppress the initial rise in blood glucose levels. Blood glucose levels at 15 min were significantly lower in the PGA group than in the Con group (P < 0.05). The plasma glucose level and NEFA level were also significantly lower in the PGA group (P < 0.05), and caecal acetic acid/total caecal SCFAs ratio was significantly increased in the PGA group (P < 0.05). Significant negative correlations existed between the caecal acetic acid/propionic acid ratio and the weight of visceral fat/BW (r = −0.57, P = 0.0318). Our results suggest that γ-PGA may effectively prevent metabolic syndrome by lowering blood glucose levels.

show abstract

Analytical approaches to poly-γ-glutamate: Quantification, molecular size determination, and stereochemistry investigation

Cited by 29 publications

References 32 publications

Poly-γ-glutamic acid produced fromBacillus licheniformisCGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Poly-γ-glutamic acid produced fromBacillus licheniformisCGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Effects of poly-γ-glutamic acid (γ-PGA) on soil nitrogen and carbon leaching and CO2 fluxes in a sandy clay loam soil

Effects of <i>γ</i>-Polyglutamic Acid on Blood Glucose and Caecal Short Chain Fatty Acids in Adult Male Mice

Contact Info

Product

Resources

About

Analytical approaches to poly-γ-glutamate: Quantification, molecular size determination, and stereochemistry investigation

Cited by 29 publications

References 32 publications

Poly-γ-glutamic acid produced fromBacillus licheniformisCGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Poly-γ-glutamic acid produced fromBacillus licheniformisCGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Effects of poly-γ-glutamic acid (γ-PGA) on soil nitrogen and carbon leaching and CO2 fluxes in a sandy clay loam soil

Effects of &lt;i&gt;γ&lt;/i&gt;-Polyglutamic Acid on Blood Glucose and Caecal Short Chain Fatty Acids in Adult Male Mice

Contact Info

Product

Resources

About

Effects of <i>γ</i>-Polyglutamic Acid on Blood Glucose and Caecal Short Chain Fatty Acids in Adult Male Mice