Analytical Lipidomics in Metabolic and Clinical Research

Hyötyläinen, Tuulia; Orešič, Matej

doi:10.1016/j.tem.2015.08.006

Cited by 25 publications

(16 citation statements)

References 12 publications

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“…1c). Lipids and related compounds such as steroids make up 17% of the library, This class of largely hydrophobic compounds is the subject of “lipidomics” and will not be discussed in detail in this contribution (27–29). …”

Section: Resultsmentioning

confidence: 99%

Evaluation of coverage, retention patterns, and selectivity of seven liquid chromatographic methods for metabolomics

Wernisch

Pennathur

2016

Anal Bioanal Chem

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Liquid chromatography-mass spectrometry (LC-MS)-based metabolomics studies require highly selective and efficient chromatographic techniques. Typically employed reversed-phase (RP) methods fail to target polar metabolites but the introduction of hydrophilic interaction liquid chromatography (HILIC) is slow due to perceived issues of reproducibility and ruggedness and a limited understanding of the complex retention mechanisms. In this study, we present a comparison of the chromatographic performance of a traditional RP-C18 column with zwitterionic, amide-, alkyl diol- and aminoalkyl-based HILIC and mixed-mode columns. Our metabolite library represents one of the largest analyte sets available and consists of 764 authentic metabolite standards including amino acids, nucleotides, sugars and other metabolites representing all major biological pathways and commonly observed exogenous metabolites (drugs). Coverage, retention patterns and selectivity of the individual methods are highly diverse even between conceptually related HILIC methods. Furthermore, we show that HILIC sorbents having highly orthogonal selectivity and specificity enhance the coverage of major metabolite groups in (semi-) targeted applications compared to RP. Finally, we discuss issues encountered in the analysis of biological samples based on results obtained with human plasma extracts. Our results demonstrate that fast and highly reproducible separations on zwitterionic columns are feasible but knowledge of analyte properties is essential to avoid chromatographic bias and exclusion of key analytes in metabolomics studies.

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Section: Resultsmentioning

confidence: 99%

Evaluation of coverage, retention patterns, and selectivity of seven liquid chromatographic methods for metabolomics

Wernisch

Pennathur

2016

Anal Bioanal Chem

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“…For example, intake of stearic acid leads to dramatically reduced visceral fat 24 , even if stearic acid can be more easily stored in liver at intermediate postprandial time points (24~48 h) than linolenic or oleic acid 25 . Palmitoleic acid and conjugated linoleic acid may prevent fat deposition in the liver 26, 27 . Furthermore, intake of DHA and arachidonic acid (ARA) can significantly reduce hepatic weight, serum TAG and TC, epididymal fat as compared to palmitic acid, which would be associated with enhanced fatty acid β-oxidation, down-regulated mRNA fatty acid synthase and sterol regulatory element binding protein-1c expression 28 .…”

Section: Discussionmentioning

confidence: 99%

“…The rats fed lard seemed more susceptible to inflammation by counting white blood cells and platelets than those of soybean and fish oils. In fish oil, a relatively high level of palmit oleic acid (16:1) could be involved in the regulation of insulin sensitivity, the inhibition of inflammation and the prevention of fat accumulation in the liver 55 . In addition, ARA can be converted to prostaglandins and leukotrienes that are important pro-inflammatory mediators and can induce ROS production 56 .…”

Section: Discussionmentioning

confidence: 99%

Fish oil diet may reduce inflammatory levels in the liver of middle-aged rats

Fan

et al. 2017

Sci Rep

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The impact of dietary soybean oil, lard and fish oil on physiological responses in middle age is little studied. In this study, we investigated the changes of oxidative stress, inflammatory cytokines, telomere length, and age-related gene expression in the liver of middle-aged rats in response to the above three fat diets. Male Sprague Dawley rats (12 months old) were fed AIN-93M diets for 3 months, in which soybean oil was equivalently replaced by lard or fish oil. As compared to the lard diet, intake of fish oil diet significantly decreased body weight gain, white blood cell count, and levels of hepatic triacylglycerol, total cholesterol, fat accumulation, low-density lipoprotein, oxidative stress and inflammatory cytokines (P < 0.05), but increased telomere length (P < 0.05). On the other hand, lard diet and soybean oil diet showed great similarity in the above variables. PCR array analysis further indicated that fish oil diet significantly down-regulated gene expression related to inflammatory response, apoptosis, DNA binding, proteostasis and telomere attrition. Differentially expressed genes were enriched in the complement and coagulation cascades pathways. Such physiological and molecular responses could be due to different fatty acid composition in fish oil, lard and soybean oil.

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“…Also, sex‐specific lipidomic changes have been described in white and brown mouse adipose [Hoene et al, ], and in the BAT response to cold stress [Marcher et al, ]. These cell, tissue, and mouse model studies, as well as numerous clinical applications [Wood, ; Zhao et al, ; Hyotylainen and Oresic, ], demonstrate the utility of lipidomic analysis to address basic scientific and biomedical questions. However, there is a lack of clarity concerning the degree to which cell culture model lipidomes represent and correspond to the in vivo contexts that they are intended to model [Lamaziere et al, ].…”

mentioning

confidence: 99%

Lipid Profiling of In Vitro Cell Models of Adipogenic Differentiation: Relationships With Mouse Adipose Tissues

Liaw

Prudovsky

Koza

et al. 2016

J of Cellular Biochemistry

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Our objective was to characterize lipid profiles in cell models of adipocyte differentiation in comparison to mouse adipose tissues in vivo. A novel lipid extraction strategy was combined with global lipid profiling using direct infusion and sequential precursor ion fragmentation, termed MS/MSALL. Perirenal and inguinal white adipose tissue and interscapular brown adipose tissues from adult C57BL/6J mice were analyzed. 3T3-L1 preadipocytes, ear mesenchymal progenitor cells, and brown adipose-derived BAT-C1 cells were also characterized. Over 3000 unique lipid species were quantified. Principal component analysis showed that perirenal versus inguinal white adipose tissues varied in lipid composition of triacyl- and diacylglycerols, sphingomyelins, glycerophospholipids and, notably, cardiolipin CL 72:3. In contrast, hexosylceramides and sphingomyelins distinguished brown from white adipose. Adipocyte differentiation models showed broad differences in lipid composition among themselves, upon adipogenic differentiation, and with adipose tissues. Palmitoyl triacylglycerides predominate in 3T3-L1 differentiation models, whereas cardiolipin CL 72:1 and SM 45:4 were abundant in brown adipose-derived cell differentiation models, respectively. MS/MSALL data suggest new lipid biomarkers for tissue-specific lipid contributions to adipogenesis, thus providing a foundation for using in vitro models of adipogenesis to reflect potential changes in adipose tissues in vivo.

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Analytical Lipidomics in Metabolic and Clinical Research

Cited by 25 publications

References 12 publications

Evaluation of coverage, retention patterns, and selectivity of seven liquid chromatographic methods for metabolomics

Evaluation of coverage, retention patterns, and selectivity of seven liquid chromatographic methods for metabolomics

Fish oil diet may reduce inflammatory levels in the liver of middle-aged rats

Lipid Profiling of In Vitro Cell Models of Adipogenic Differentiation: Relationships With Mouse Adipose Tissues

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