Analytical method to examine the effects of carbon dioxide lasers on skin: A study using wrinkles induced in hairless mice

Fujimura, Tsutomu; Moriwaki, Shigeru; Tsukahara, Kazue; Imokawa, Genji; Yamada, Akira; Imayama, Syuhei

doi:10.1002/lsm.1061

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…Histological examinations performed in this study did prove collagen increase, confirming that skin elasticity measurement was an objective and non‐invasive method to evaluate the efficiency of laser non‐ablative resurfacing. Fujimura et al (19) also measured the changes of mouse skin elasticity after CO 2 laser irradiation and found skin elasticity recovery was incomplete even after 49 days, which was not consistent with our results of only 7 days for recovery. Therefore, the advantage of non‐ablative technique, minimal healing time was shown compared with several weeks needed for ablative technique.…”

Section: Discussioncontrasting

confidence: 95%

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Comparison of histologic, biochemical, and mechanical properties of murine skin treated with the 1064‐nm and 1320‐nm Nd:YAG lasers

Dang

Ren

Liu

et al. 2005

Experimental Dermatology

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The goal of this study was to compare the effects of the Q-switched 1064-nm Nd:YAG laser and the 1320-nm Nd:YAG laser non-ablative treatments on mouse skin in vivo. Skin elasticity measurements were carried out with a Reviscometer, and skin samples were taken for histological study, hydroxyproline content assay and estimation of collagen type I and III. By the second month after non-ablative treatments, the 1064-nm laser treatment resulted in an average of 25% greater improvement of skin elasticity, 6% more increase of dermal thickness, and 11% higher synthesis of hydroxyproline than the 1320-nm laser. Collagen type III increased markedly after the 1064-nm laser treatment whereas more collagen type I was elicited by the 1320-nm laser. Our results demonstrated that the 1064-nm laser was more effective than the 1320-nm Nd:YAG laser in non-ablative treatments, but the results needed to be confirmed in humans. It appeared that photo-mechanic reaction could cause more collagen type III synthesis whereas the photo-thermal effect was in favor of the formation of collagen type I.

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Section: Discussioncontrasting

confidence: 95%

“…Further research should perform a longer period to better assess the durability of collagen formation. Previous study described dermal thickness increased mainly due to the marked increase in cysts in the lower dermal layer (19), but in our results the increase of dermal thickness appeared to be mostly as a result of collagen increase.…”

Section: Discussioncontrasting

confidence: 81%

Comparison of histologic, biochemical, and mechanical properties of murine skin treated with the 1064‐nm and 1320‐nm Nd:YAG lasers

Dang

Ren

Liu

et al. 2005

Experimental Dermatology

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“…In chronically UVA-exposed rat hind limb skin, there is also a tortuous deformation, together with fraying, of elastic fibers similar to that observed in chronically UVB-exposed skin, although the magnitude of the decreased linearity is greater in the UVB-exposed skin than in the UVA-exposed skin [ 15 ]. The epistatic and close interrelationship among the three-dimensional alterations of elastic fiber networks, the loss of skin elasticity, and wrinkle formation are also corroborated by other animal studies in which wrinkles or sagging were ameliorated by a CO 2 laser [ 24 , 25 ] and by retinoic acid treatment [ 26 ], or were accelerated by ovariectomy [ 13 , 27 ] in concert with concomitant repair or impairment, respectively, in the straight configuration of dermal elastic fibers and skin elastic properties.…”

Section: Skin Elastic Features Are Highly Attributable To the Thresupporting

confidence: 66%

Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging I: Reduced Skin Elasticity, Highly Associated with Enhanced Dermal Elastase Activity, Triggers Wrinkling and Sagging

Imokawa

Ishida

2015

IJMS

Self Cite

137

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The repetitive exposure of skin to ultraviolet B (UVB) preferentially elicits wrinkling while ultraviolet A (UVA) predominantly elicits sagging. In chronically UVB or UVA-exposed rat skin there is a similar tortuous deformation of elastic fibers together with decreased skin elasticity, whose magnitudes are greater in UVB-exposed skin than in UVA-exposed skin. Comparison of skin elasticity with the activity of matrix metalloproteinases (MMPs) in the dermis of ovariectomized rats after UVB or UVA irradiation demonstrates that skin elasticity is more significantly decreased in ovariectomized rats than in sham-operated rats, which is accompanied by a reciprocal increase in elastase activity but not in the activities of collagenases I or IV. Clinical studies using animal skin and human facial skin demonstrated that topical treatment with a specific inhibitor or an inhibitory extract of skin fibroblast-derived elastase distinctly attenuates UVB and sunlight-induced formation of wrinkling. Our results strongly indicated that the upregulated activity of skin fibroblast-derived elastase plays a pivotal role in wrinkling and/or sagging of the skin via the impairment of elastic fiber configuration and the subsequent loss of skin elasticity.

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“…Consequently, we assumed other factors maybe also contribute the increase of dermal thickness. Fujimura ever observed the similar thing in his study and explained the increase of skin thickness may be mainly due to the marked increase in cysts 16 . The mechanism was unclear and needed the further study.…”

Section: Discussionmentioning

confidence: 84%

In vivo experiment study of nonablative photorejuvenation by using a 595-nm pulsed dye laser

Dang

Ren

et al. 2005

SPIE Proceedings

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The pulsed dye laser (PDL) has a history of producing safe and effective clearance of dermal vascular lesions; however, non-ablative treatments of rhytids with 595nm PDL are seldom studied. The purpose of our research is to evaluate the changes of skin elasticity, histology and the amount of hydroxyproline after 595nm PDL non-ablative rejuvenation and to offer references for effective clinical treatments. Forty KM mice were used for this experiment. Laser parameters were as follows: an energy fluence of 8 to 12J/cm 2 , a pulse duration of 10ms, and a spot size of 7mm with 10% overlap. Skin elasticity was measured using Reviscometer ® RVM 600. Specimens were sectioned for hematoxylin-eosin and Van-Gieson staining, and dermal thickness was recorded in an ocular micrometer. The amount of hydroxyproline in the dermis was quantified by the biochemical method. No marked side effects such as blister and purpura were noted during laser treatments. New collagen synthesized with an improvement in the organization of collagen fibrils. The 12 J/cm 2 group improved skin elasticity by 31.7%, dermal thickness by 25.3% and the amount of hydroxyproline by 55.9%. There were the good correlations between dermal thickness and the amount of hydroxyproline. Therefore 595nm PDL non-ablative photo-rejuvenation is a safe and effective method for wrinkle reduction. And the energy level of 12 J/cm 2 has the greatest effect in improving skin mechanical properties and accelerating new collagen formation.

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Analytical method to examine the effects of carbon dioxide lasers on skin: A study using wrinkles induced in hairless mice

Abstract: An abnormal state characterized by excessive collagen regeneration and other changes in the dermis occur concomitantly with wrinkle smoothing.

Cited by 8 publications

References 24 publications

Comparison of histologic, biochemical, and mechanical properties of murine skin treated with the 1064‐nm and 1320‐nm Nd:YAG lasers

Comparison of histologic, biochemical, and mechanical properties of murine skin treated with the 1064‐nm and 1320‐nm Nd:YAG lasers

Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging I: Reduced Skin Elasticity, Highly Associated with Enhanced Dermal Elastase Activity, Triggers Wrinkling and Sagging

In vivo experiment study of nonablative photorejuvenation by using a 595-nm pulsed dye laser

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