Generally, the approximate pH of honey is 4.0 (Ouchemoukh et al., 2007). Several physicochemical properties show the differences in the chemical composition of honey (Bogdanov et al., 1999), and its sensorial, chemical, physical, and microbiological characteristics are investigated to determine its quality. The main criteria are moisture content, ash content, electrical conductivity, diastase activity, free acidity, 5-hydroxymethyl furfuraldehyde (HMF) content, and reducing and non-reducing sugars (Gomes et al., 2010). Honey production has developed well in Turkey thanks to its geographical and climatic conditions. As an important agricultural activity, the beekeeping has a history of thousands of years in Turkey (Kahraman et al., 2010). The Black Sea Region ranks the first in terms of production. In 2016, 1,786,996 tons of honey was produced globally and Turkey ranked the second after China with 105,532 tons (Food and Agriculture Organization, 2020). Turkey has favorable conditions for honey production like climate, topographical structure, and a wide variety of plant flora (Gül & Pehlivan, 2018;Sari & Ayyildiz, 2012;Tezcan et al., 2011). The appearance, nutritional value, composition, and flavor of honey are affected by its botanical origins and the geographic location of beehives (Gül & Pehlivan, 2018;Tezcan et al., 2011).The composition of honey can vary depending on its origin. For example, the mineral sources primarily originate from its raw materials (nectar and honeydew) and the pollen grains (Madejczyk & Baralkiewicz, 2008;Pohl et al., 2011). Carbohydrates (glucose and fructose) account for about 80-95% of the matrix, while the total mineral content in honey should not exceed 1%. The second most important content in honey is water, and its ratio depends on several environmental factors during production such as weather and humidity (Alvarez-Suarez et al., 2010;Bogdanov et al., 2008). Besides, water content affects the basic physical characteristics of honey, such as viscosity, maturity, and crystallization (Machado De-Melo et al., 2018). Viscosity of ripe honey is higher, with water content of below 22%, leading to non growth of microorganisms and longer shelf life of ripe honey (Aparna & Rajalakshmi, 1999), and significantly improving the re-ripe honey market acceptance and increasing the beekeepers' profit given the the production rate (Guo et al., 2019). It is known that fermentation is another factor that affects the honey composition when moisture exceeds 18%, especially after a long storage time (Bogdanov et al., 2008). Proteins, minerals, enzymes, vitamins, organic acids, and phenolic compounds are minor components of honey (Pohl et al., 2011). Protein, enzyme, and vitamin contents of honey are obtained from the